LOCUS       sequence-318774-        2880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term 8xHis 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 4 pCOLI_A_3c_C_HIS
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     375..394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     628..645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    660..799
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(725..744)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     885..907
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(901..1092)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1329..1348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1329..1347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1385..1407
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1467..1490
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1497..1520
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(1614..1632)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1628..1675
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1740..1831
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2696..2735
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      join(2766..2880,1..474)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
       61 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttgccg
      181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      661 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
      721 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
      781 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      841 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      901 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
      961 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     1021 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     1081 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggt aatgataccg
     1141 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     1201 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     1261 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttggcaccat gcatcgatcc
     1321 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctccg gctccggaaa
     1381 taattttgtt taactttaag aaggagactg gatccgaatc tagagcctgc agtctcgaca
     1441 agcttggaag cggatctgga tctggactgg aagttctgtt ccaggggccc ggtagccatc
     1501 atcatcatca tcaccatcac taataagtcg agaagtacta gaggatgtac aacgcgtgcg
     1561 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     1621 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     1681 aggaggaact atatcgggat tagcccgcct aatgagcggg ctttttttta attcccctat
     1741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1801 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1861 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa
     1921 agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa
     1981 tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt
     2041 caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg
     2101 acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca
     2161 ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa
     2221 cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt
     2281 gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc
     2341 cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa cattacgcaa
     2401 actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag actggcttga
     2461 agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct ggtttattgc
     2521 tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac ttggaccaga
     2581 tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa ctatggacga
     2641 acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt aaaccgatac
     2701 aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa gatcaaagga
     2761 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2821 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
//
