LOCUS       sequence-318777-        3507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term GST 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 10 pCOLI_A_3C_C_GST
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      55..643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     544..563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     797..814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    829..968
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(894..913)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1054..1076
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1070..1261)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1498..1517
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1498..1516
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1554..1576
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1636..1659
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1666..2319
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2274..2296
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(2410..2428)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2424..2471
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2536..2627
                     /gene="bla"
                     /label=AmpR promoter
     terminator      join(3492..3507,1..24)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ccttttggag cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga
       61 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      181 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
      241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttct
      541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      601 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
      841 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
      901 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
      961 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     1021 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     1081 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     1141 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     1201 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     1261 ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta atgataccga tgaaacgaga
     1321 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     1381 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     1441 ccagcgcttc gttaatacag atgtaggtgt tggcaccatg catcgatccc gcgaaattaa
     1501 tacgactcac tatagggaga ccacaacggt ttccctccgg ctccggaaat aattttgttt
     1561 aactttaaga aggagactgg atccgaatct agagcctgca gtctcgacaa gcttggaagc
     1621 ggatctggat ctggactgga agttctgttc caggggcccg gtagctcccc tatactaggt
     1681 tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata tcttgaagaa
     1741 aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt
     1801 gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca
     1861 cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca
     1921 aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt
     1981 tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta
     2041 cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat
     2101 catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac
     2161 ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc
     2221 ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg
     2281 caagccacgt ttggtggtgg cgaccatcct ccaaaataat aagtcgagaa gtactagagg
     2341 atgtacaacg cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     2401 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     2461 ggggtttttt gctgaaagga ggaactatat cgggattagc ccgcctaatg agcgggcttt
     2521 tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2581 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2701 cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga gtgggataca
     2761 tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc
     2821 caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt gtagatgcgg
     2881 ggcaagagca actcggacga cgaatacact attcgcagaa tgacttggtt gaatactccc
     2941 cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt agtgccgcca
     3001 taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt ggaccgaagg
     3061 aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac cgttgggaac
     3121 cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct gcggcaatgg
     3181 caacaacatt acgcaaacta ttaactggcg aactacttac tctggcttca cggcaacaat
     3241 taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg gcacttcctg
     3301 ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg ggtatcattg
     3361 ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg acgggtagtc
     3421 aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca ctgattaagc
     3481 attggtaaac cgatacaatt aaaggct
//
