LOCUS       sequence-318779-        2904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term CBP non-cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 14 pCOLI_A_C_CBP
FEATURES             Location/Qualifiers
     source          1..2904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1444..1463
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1444..1462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1500..1522
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1582..1659
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     complement(1753..1771)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1767..1814
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1879..1970
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2835..2874
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga taccgatgaa
     1261 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     1321 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     1381 tcaatgccag cgcttcgtta atacagatgt aggtgttggc accatgcatc gatcccgcga
     1441 aattaatacg actcactata gggagaccac aacggtttcc ctccggctcc ggaaataatt
     1501 ttgtttaact ttaagaagga gactggatcc gaatctagag cctgcagtct cgacaagctt
     1561 ggaagcggat ctggatctgg aaagcgacga tggaaaaaga atttcatagc cgtctcagca
     1621 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttt aataagtcga gaagtactag
     1681 aggatgtaca acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
     1741 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1801 tgaggggttt tttgctgaaa ggaggaacta tatcgggatt agcccgccta atgagcgggc
     1861 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2041 acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca cgagtgggat
     2101 acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct gaagagcgtt
     2161 ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc cgtgtagatg
     2221 cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg gttgaatact
     2281 ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta tgtagtgccg
     2341 ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc ggtggaccga
     2401 aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt gaccgttggg
     2461 aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg cctgcggcaa
     2521 tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct tcacggcaac
     2581 aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt tcggcacttc
     2641 ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca cggggtatca
     2701 ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac acgacgggta
     2761 gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct tcactgatta
     2821 agcattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacggacc
     2881 ggtagaaaag atcaaaggat cttc
//
