LOCUS       sequence-318780-        3044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term HA 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 15 pCOLI_A_HA
FEATURES             Location/Qualifiers
     source          1..3044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      283..322
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1127..1266
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1192..1211)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1352..1374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1368..1559)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1796..1815
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1796..1814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1852..1874
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1894..1911
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1924..1950
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1957..1983
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1990..2016
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2029..2052
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2245..2263)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2259..2306
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2371..2462
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaactactta ctctggcttc acggcaacaa ttaatagact ggcttgaagc ggataaagtt
       61 gcaggaccac tactgcgttc ggcacttcct gctggctggt ttattgctga taaatctggg
      121 gcaggagagc gtggttcacg gggtatcatt gccgcacttg gaccagatgg taagccttcc
      181 cgtatcgtag ttatctacac gacgggtagt caggcaacta tggacgaacg aaatagacag
      241 attgctgaaa taggggcttc actgattaag cattggtaaa ccgatacaat taaaggctcc
      301 ttttggagcc tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     1141 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
     1201 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     1261 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1321 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1381 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     1441 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     1501 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     1561 gatgcctccg tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgagaga
     1621 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     1681 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     1741 agcgcttcgt taatacagat gtaggtgttg gcaccatgca tcgatcccgc gaaattaata
     1801 cgactcacta tagggagacc acaacggttt ccctccggct ccggaaataa ttttgtttaa
     1861 ctttaagaag gagatataca tatggggagt tcgcaccatc atcaccacca tagctctggc
     1921 tcctatccgt atgacgtgcc agactatgct ggtagctatc cttacgatgt ccccgattat
     1981 gcgggatctt atccatacga tgtgccagac tatgcaagtt caggttccct ggaagttctg
     2041 ttccaggggc ccggatcccg gtccgacata tgagcgcgcg gaattcaaag gcctacgtcg
     2101 acgagctcac tagtcgcggc cgctttcgaa tctagagcct gcagtctcga caagcttgtc
     2161 gagaagtact agaggatgta caacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa
     2221 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     2281 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatcggga ttagcccgcc
     2341 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata
     2401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2521 ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg
     2581 cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc
     2641 ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat
     2701 cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat acactattcg cagaatgact
     2761 tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat
     2821 tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca
     2881 tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc
     2941 ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa
     3001 tgcctgcggc aatggcaaca acattacgca aactattaac tggc
//
