LOCUS       sequence-318794-       15760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ABE7.10-N-AIDmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15760)
  AUTHORS   Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu
            H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang 
            Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D
  TITLE     Dual base editor catalyzes both cytosine and adenine base 
            conversions in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y.
  PUBMED    32483363
REFERENCE   2  (bases 1 to 15760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y."
COMMENT     Sequence Label: Lenti ABE7.10-N-AIDmax
FEATURES             Location/Qualifiers
     source          1..15760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1481..1525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2052..2263
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2329..2349
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2905..2925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4129..4152,4153..4155,4156..8229)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4129..8229
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8230..8277
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8293..8349
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8356..8358,8359..8361,8362..9072)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8356..8358,8359..8361,8362..9072)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8662..8681)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9009..9030
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9108..9607
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9143..9166)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9540..9559
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9620..10219
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9620..9639)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10116..10136
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10235..10823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10288..10308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10706..10717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10895..11128
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11154..11171)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11160..11384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11430..11858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11517..11536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11727..11748
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11867..11887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11872..12201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12052..12187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12114..12133
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12249..12296
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12315..12689
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12819..12940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12856..12875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12910..12929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12989..13005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12989..13005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13002..13024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13013..13029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13037..13043,13044..13061,13062..13067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13082..13103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13220..13237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13391..13979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13471..13490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14150..14941,14942..15010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14773..14792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15011..15115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15190..15209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        15381..15760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttaaggtacc gaattcgata tcgctagcta ggtcttgaaa ggagtgggaa ttggctccgg
     2041 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     2101 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     2161 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     2221 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggggcgcg cctctagagc
     2281 gatcgcgcca ccatgaagag gaccgccgat ggctctgagt tcgagagccc caagaagaag
     2341 cggaaggtgg acagcctgct gatgaaccgg agaaagttcc tgtatcagtt taagaatgtg
     2401 aggtgggcaa agggcaggcg cgagacctac ctgtgctatg tggtgaagcg gagagatagc
     2461 gccacatctt tcagcctgga ctttggctac ctgcggaaca agaatggctg ccacgtggag
     2521 ctgctgtttc tgagatacat ctccgactgg gatctggacc caggcaggtg ttatcgcgtg
     2581 acctggttca catcctggtc tccctgctac gattgtgccc ggcacgtggc cgactttctg
     2641 agaggcaacc ctaatctgtc tctgaggatc ttcaccgccc gcctgtattt ttgcgaggat
     2701 aggaaggccg agccagaggg actgaggcgc ctgcacaggg ccggcgtgca gatcgccatc
     2761 atgacattca aggactactt ttattgttgg aacaccttcg tggagaatca cgagcggaca
     2821 tttaaggcct gggagggact gcacgagaac tccgtgcggc tgtctagaca gctgcggaga
     2881 atcctgctgc caagcggcgg ctcccccaag aagaagcgga aagtgggcag ctccggctcc
     2941 agcgaggtgg agttcagcca cgagtactgg atgagacacg ccctgaccct ggctaagaga
     3001 gcttgggatg agagagaggt gcccgtggga gctgttctgg ttcataacaa cagggtgatc
     3061 ggcgagggat ggaacagacc tatcgggaga cacgacccaa ccgctcatgc tgaaatcatg
     3121 gccctgagac aaggagggct ggtgatgcaa aattacagac tgatcgacgc aaccctgtac
     3181 gtgaccctgg agccttgtgt gatgtgcgca ggagcaatga tccactccag aatcggcaga
     3241 gtggtgttcg gagctagaga tgccaaaacc ggagccgctg gaagcctgat ggacgttctg
     3301 catcaccccg gaatgaatca cagagtggag ataaccgagg gcattctggc cgacgagtgt
     3361 gctgctctgc tgtctgattt cttcagaatg agaaggcagg aaatcaaggc ccagaaaaag
     3421 gcccaaagca gcaccgacag cggaggatct agcggaggat caagcggaag cgagactcct
     3481 ggaaccagcg aaagcgcaac cccagaaagc agcggaggaa gtagcggagg aagctcagaa
     3541 gtcgagttca gccatgagta ttggatgaga catgctctga ccctggcaaa gagagcaaga
     3601 gacgagagag aggtcccagt gggagcagtt ctggtgctga acaacagagt gatcggggag
     3661 gggtggaaca gagcaatcgg actgcacgac cctacagcac acgcagaaat aatggcactg
     3721 agacaagggg ggctcgtgat gcaaaactac aggctgatcg acgccaccct gtacgtcaca
     3781 tttgagccct gtgtgatgtg tgccggagcc atgattcaca gtagaatcgg ccgggtggtg
     3841 ttcggtgtga gaaacgctaa aacaggcgcc gccggaagcc tgatggatgt tctgcattac
     3901 cccggcatga atcaccgggt ggagatcaca gagggcatcc tggctgacga atgtgccgct
     3961 ctgctgtgtt acttcttcag aatgccccga caagtgttca acgcccagaa gaaagcccag
     4021 tcaagcaccg actctggcgg atctagcggt ggatctagcg gctctgagac ccctggaaca
     4081 tccgaatccg ccactccaga gagcagcgga ggctcttctg gaggatcaga caagaagtac
     4141 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     4201 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     4261 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     4321 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     4381 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     4441 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     4501 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     4561 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     4621 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     4681 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     4741 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     4801 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     4861 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     4921 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     4981 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     5041 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     5101 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     5161 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     5221 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     5281 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     5341 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     5401 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     5461 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     5521 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     5581 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     5641 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     5701 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     5761 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     5821 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     5881 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     5941 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     6001 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     6061 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     6121 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     6181 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     6241 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     6301 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     6361 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     6421 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     6481 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     6541 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     6601 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     6661 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     6721 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     6781 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     6841 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     6901 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     6961 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     7021 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     7081 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     7141 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     7201 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     7261 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     7321 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     7381 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     7441 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     7501 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     7561 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     7621 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     7681 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     7741 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     7801 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     7861 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     7921 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     7981 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     8041 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     8101 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     8161 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     8221 ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga
     8281 tccggcagcg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat
     8341 cctggaccgc tcgagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     8401 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     8461 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     8521 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     8581 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     8641 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     8701 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     8761 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     8821 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     8881 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     8941 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     9001 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9061 gagctgtaca agtactcaga tctggagctc aagtgagcta gcctaccggg taggggaggc
     9121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     9181 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     9241 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     9301 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     9361 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     9421 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     9481 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     9541 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     9601 cctttcggaa ttcgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9661 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9721 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9781 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     9841 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9901 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9961 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca
    10021 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
    10081 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
    10141 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
    10201 ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
    10261 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10321 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10381 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10441 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10501 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10561 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10621 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
    10681 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10741 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    10801 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
    10861 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    10921 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    10981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    11041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    11101 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
    11161 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
    11221 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
    11281 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
    11341 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
    11401 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
    11461 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    11521 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    11581 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    11641 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    11701 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    11761 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    11821 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
    11881 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    11941 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    12001 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    12061 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    12121 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
    12181 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
    12241 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
    12301 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
    12361 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
    12421 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
    12481 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
    12541 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
    12601 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
    12661 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
    12721 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    12781 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    12841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12901 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    12961 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    13021 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    13081 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    13141 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    13201 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13561 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13801 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    13861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    13921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    13981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    14041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    14101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    14161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    14221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    14281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    14341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    14401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    14461 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    14521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    14581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    14641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    14701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    14761 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    14821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    14881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    14941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    15001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    15061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    15121 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    15181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    15241 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    15301 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    15361 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
    15421 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    15481 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    15541 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    15601 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    15661 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    15721 ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
