LOCUS       sequence-318810-       14934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hyeA3A-BE4max.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Zhang X, Chen L, Zhu B, Wang L, Chen C, Hong M, Huang Y, Li H, Han 
            H, Cai B, Yu W, Yin S, Yang L, Yang Z, Liu M, Zhang Y, Mao Z, Wu Y, 
            Liu M, Li D
  TITLE     Increasing the efficiency and targeting range of cytidine base 
            editors through fusion of a single-stranded DNA-binding protein 
            domain.
  JOURNAL   Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 
            10.1038/s41556-020-0518-8. Epub 2020 May 11.
  PUBMED    32393889
REFERENCE   2  (bases 1 to 14934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-020-0518-8"; journalName: "Nat Cell Biol"; date: 
            "2020-06"; volume: "22"; issue: "6"; pages: "740-750"
COMMENT     Sequence Label: Lenti-117G-hyeA3A-BE4max
FEATURES             Location/Qualifiers
     source          1..14934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        125..172
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             191..565
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    695..816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(732..751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     786..805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(865..881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(865..881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(878..900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    889..905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(913..919,920..937,938..943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    958..979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1096..1113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1267..1855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1347..1366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2026..2817,2818..2886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2649..2668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2887..2991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3066..3085)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3257..3636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3638..3836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3791..3811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3854..4034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4081..4206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4699..4932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5117..5161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5459..5576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5622..5870
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5622..5642
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5793..5812
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5892..5967
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6024..6235
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6301..6321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7450..7473,7474..7476,7477..11550)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7450..11550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11551..11598
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             11608..11661
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11662..11910
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             11941..12189
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             12232..12252
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12262..12318
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(12319..12321,12322..12324,12325..13038)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12319..12321,12322..12324,12325..13035)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12625..12644)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12972..12993
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    13045..13633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13098..13118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13516..13527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13705..13938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(13964..13981)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13970..14194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14240..14668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14327..14346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14537..14558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14677..14697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(14682..14934,1..77)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(14862..14934,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     join(14924..14934,1..9)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
       61 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
      121 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
      181 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
      241 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
      301 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
      361 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
      421 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
      481 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
      541 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
      601 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
      661 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
      721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
      841 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      901 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      961 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1021 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1081 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1141 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1201 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1261 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1321 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1381 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1441 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1501 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1621 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1681 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1741 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1801 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1861 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1921 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1981 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2041 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2101 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2161 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2221 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2281 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2341 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2401 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2461 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2521 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2581 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2641 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2701 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2761 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2821 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2881 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2941 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3001 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     3061 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     3121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     3181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     3241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     3361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     3541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     3601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     3661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     3721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     3781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     3841 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4021 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     4081 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4141 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4201 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4261 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     4321 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     4381 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     4441 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     4501 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     4561 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     4621 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     4681 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     4741 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     4801 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     4861 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     4921 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     4981 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5041 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5101 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5161 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5221 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5281 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     5341 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     5401 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     5461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     5521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     5581 tttattacag ggacagcaga gatccagttt ggttaattaa tgagggccta tttcccatga
     5641 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     5701 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     5761 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     5821 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggctattggt
     5881 caaggcaagg cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     5941 cttgaaaaag tggcaccgag tcggtgcttt ttttgctagc taggtcttga aaggagtggg
     6001 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     6061 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     6121 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     6181 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggggcg
     6241 cgcctctaga gcgatcgcgc caccatgaag aggaccgccg atggctctga gttcgagagc
     6301 cccaagaaga agcggaaggt ggaggcatct ccagcaagcg gaccaaggca cctgatggac
     6361 ccccacatct tcacctctaa ctttaacaat ggcatcggca ggcacaagac atacctgtgc
     6421 tatgaggtgg agcgcctgga caacggcacc agcgtgaaga tggatcagca cagaggcttc
     6481 ctgcacggcc aggccaagaa tctgctgtgc ggcttctacg gccggcacgc agagctgaga
     6541 tttctggacc tggtgcctag cctgcagctg gatccagccc agatctatag ggtgacctgg
     6601 ttcatcagct ggtccccatg cttttcctgg ggatgtgcag gagaggtgcg cgccttcctg
     6661 caggagaata cacacgtgcg gctgagaatc tttgccgccc ggatctacga ctatgatcct
     6721 ctgtacaagg aggccctgca gatgctgaga gacgcaggag cccaggtgtc catcatgacc
     6781 tatgatgagt tcaagcactg ctgggacaca tttgtggatc accagggctg tccctttcag
     6841 ccttgggacg gactggatga gcactcccag gccctgtctg gcaggctgag ggccatcctg
     6901 cagaaccagg gcaatagcgg aggatctagc ggaggatcaa gcggaagcga gactcctgga
     6961 accagcgaaa gcgcaacccc agaaagcagc ggaggaagta gcggaggaag catggcaatg
     7021 cagatgcagc ttgaagcaaa tgcagatact tcagtggaag aagaaagctt tggcccacaa
     7081 cccatttcac ggttagagca gtgtggcata aatgccaacg atgtgaagaa attggaagaa
     7141 gctggattcc atactgtgga ggctgttgcc tatgcgccaa agaaggagct aataaatatt
     7201 aagggaatta gtgaagccaa agctgataaa attctggctg aggcagctaa attagttcca
     7261 atgggtttca ccactgcaac tgaattccac caaaggcggt cagagatcat acagattact
     7321 actggctcca aagagcttga caaactactt caatctggcg gatctagcgg tggatctagc
     7381 ggctctgaga cccctggaac atccgaatcc gccactccag agagcagcgg aggctcttct
     7441 ggaggatcag acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
     7501 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     7561 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     7621 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     7681 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     7741 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     7801 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     7861 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     7921 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     7981 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     8041 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     8101 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     8161 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     8221 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     8281 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     8341 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     8401 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     8461 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     8521 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     8581 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     8641 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     8701 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     8761 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     8821 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     8881 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     8941 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     9001 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     9061 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     9121 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     9181 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     9241 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     9301 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     9361 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     9421 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     9481 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     9541 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     9601 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     9661 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     9721 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     9781 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     9841 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     9901 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     9961 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
    10021 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
    10081 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
    10141 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
    10201 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
    10261 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
    10321 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
    10381 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
    10441 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
    10501 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
    10561 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
    10621 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
    10681 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
    10741 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
    10801 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
    10861 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
    10921 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
    10981 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
    11041 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
    11101 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
    11161 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
    11221 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
    11281 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
    11341 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
    11401 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
    11461 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
    11521 cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc
    11581 caggcaaaaa agaaaaaggg atccggagag ggcagaggaa gtctgctaac atgcggtgac
    11641 gtcgaggaga atcctggccc aactaatctg agcgacatca ttgagaagga gactgggaaa
    11701 cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc
    11761 aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat
    11821 gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat
    11881 tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc tggaggcagc
    11941 accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat ccaggagagc
    12001 atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga gagcgatatc
    12061 ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct gacatccgac
    12121 gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga gaacaaaatc
    12181 aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
    12241 aagaggaaag tcggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
    12301 gaggagaacc ctggacctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
    12361 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
    12421 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
    12481 cccgtgccct ggcccaccct cgtgaccacc ctgacctatg gagtgcagtg cttcagccgc
    12541 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
    12601 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
    12661 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
    12721 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
    12781 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
    12841 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
    12901 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
    12961 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
    13021 gacgagctgt acaagtaggt taacaatcaa cctctggatt acaaaatttg tgaaagattg
    13081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    13141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    13201 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    13261 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    13321 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    13381 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    13441 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    13501 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    13561 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    13621 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    13681 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    13741 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    13801 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    13861 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    13921 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
    13981 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    14041 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    14101 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    14161 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    14221 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    14281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    14341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    14401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    14461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    14521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    14581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    14641 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    14701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    14761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    14821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    14881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcag
//
