LOCUS       sequence-318811-       14620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AID-BEmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14620)
  AUTHORS   Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu
            H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang 
            Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D
  TITLE     Dual base editor catalyzes both cytosine and adenine base 
            conversions in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y.
  PUBMED    32483363
REFERENCE   2  (bases 1 to 14620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 
            10.1038/s41587-020-0527-y."
COMMENT     Sequence Label: Lenti AID-BEmax
FEATURES             Location/Qualifiers
     source          1..14620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1041..1060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1232..1611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1613..1811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1766..1786
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1829..2009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2056..2181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2674..2907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3092..3136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3434..3551
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3663..3874
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3940..3960
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4600..4623,4624..4626,4627..8700)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4600..8700
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8701..8748
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8764..8820
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8827..8829,8830..8832,8833..9543)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8827..8829,8830..8832,8833..9543)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9133..9152)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9480..9501
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        9579..10078
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9614..9637)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10011..10030
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10091..10690
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10091..10110)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10587..10607
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10706..11294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10759..10779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11177..11188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11366..11599
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11625..11642)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11631..11855
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11901..12329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11988..12007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12198..12219
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12338..12358)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12343..12672
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12523..12658
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12585..12604
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12720..12767
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12786..13160
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13290..13411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13327..13346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13381..13400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13460..13476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13460..13476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13473..13495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13484..13500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13508..13514,13515..13532,13533..13538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13553..13574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13691..13708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13862..14450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13942..13961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     1021 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     1081 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     1141 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     1201 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     1261 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1321 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1381 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1441 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1501 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1561 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1621 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1681 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1741 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     1801 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     1861 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1921 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1981 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     2041 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     2101 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     2161 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     2221 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     2281 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     2341 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     2401 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     2461 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     2521 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2581 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2641 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2701 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2761 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2821 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2881 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2941 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     3001 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     3061 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     3121 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     3181 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     3241 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     3301 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     3361 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     3421 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     3481 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3541 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaaggtac
     3601 cgaattcgat atcgctagct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     3661 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3721 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3781 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     3841 tctttttcgc aacgggtttg ccgccagaac acaggggcgc gcctctagag cgatcgcgcc
     3901 accatgaaga ggaccgccga tggctctgag ttcgagagcc ccaagaagaa gcggaaggtg
     3961 gacagcctgc tgatgaaccg gagaaagttc ctgtatcagt ttaagaatgt gaggtgggca
     4021 aagggcaggc gcgagaccta cctgtgctat gtggtgaagc ggagagatag cgccacatct
     4081 ttcagcctgg actttggcta cctgcggaac aagaatggct gccacgtgga gctgctgttt
     4141 ctgagataca tctccgactg ggatctggac ccaggcaggt gttatcgcgt gacctggttc
     4201 acatcctggt ctccctgcta cgattgtgcc cggcacgtgg ccgactttct gagaggcaac
     4261 cctaatctgt ctctgaggat cttcaccgcc cgcctgtatt tttgcgagga taggaaggcc
     4321 gagccagagg gactgaggcg cctgcacagg gccggcgtgc agatcgccat catgacattc
     4381 aaggactact tttattgttg gaacaccttc gtggagaatc acgagcggac atttaaggcc
     4441 tgggagggac tgcacgagaa ctccgtgcgg ctgtctagac agctgcggag aatcctgctg
     4501 ccaagcggag gatctagcgg aggatctagc ggctctgaga cccctggaac atccgaatcc
     4561 gccactccag agagcagcgg aggctcttct ggaggatcag acaagaagta cagcatcggc
     4621 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     4681 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     4741 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     4801 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     4861 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     4921 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     4981 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     5041 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     5101 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     5161 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     5221 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     5281 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     5341 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     5401 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     5461 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     5521 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     5581 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     5641 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     5701 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     5761 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     5821 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     5881 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     5941 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     6001 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     6061 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     6121 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     6181 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     6241 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     6301 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     6361 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     6421 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     6481 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     6541 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     6601 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     6661 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     6721 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     6781 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     6841 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     6901 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     6961 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     7021 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     7081 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     7141 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     7201 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     7261 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     7321 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     7381 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     7441 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     7501 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     7561 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     7621 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     7681 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     7741 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     7801 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     7861 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     7921 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     7981 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     8041 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     8101 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     8161 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     8221 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     8281 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     8341 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     8401 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     8461 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     8521 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     8581 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     8641 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     8701 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atccggcagc
     8761 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     8821 ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     8881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     8941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     9001 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     9061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     9121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     9181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     9241 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     9301 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     9361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     9421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     9481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     9541 aagtactcag atctggagct caagtgagct agcctaccgg gtaggggagg cgcttttccc
     9601 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     9661 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     9721 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     9781 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     9841 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     9901 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     9961 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
    10021 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgga
    10081 attcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    10141 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    10201 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    10261 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    10321 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    10381 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    10441 gcccaaggag cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa
    10501 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    10561 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    10621 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    10681 cggtgcctga acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
    10741 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    10801 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    10861 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    10921 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    10981 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    11041 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    11101 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    11161 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    11221 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    11281 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
    11341 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
    11401 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    11461 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    11521 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    11581 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
    11641 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    11701 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    11761 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    11821 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
    11881 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    11941 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    12001 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    12061 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    12121 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    12181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    12241 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    12301 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
    12361 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    12421 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    12481 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    12541 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    12601 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    12661 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
    12721 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
    12781 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
    12841 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
    12901 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
    12961 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
    13021 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
    13081 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
    13141 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
    13201 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    13261 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    13321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    13381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    13441 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    13501 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    13561 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    13621 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    13681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    14041 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    14101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    14161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    14221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    14281 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    14341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    14401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    14461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    14521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    14581 aatcaatcta aagtatatat gagtaaactt ggtctgacag
//
