LOCUS       sequence-318827-       11435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 splice variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-GFP-v2 IQSEC1 WT
FEATURES             Location/Qualifiers
     source          1..11435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        40..343
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        344..547
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     498..518
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      586..595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(592..594,595..597,598..1308)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(592..594,595..597,598..1308)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(637..658)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(898..917)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1245..1266
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4417..5005
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4470..4490)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4888..4899)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5077..5310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5388..5509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5425..5444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5479..5498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5549..5684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5704..5723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5705..5723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5733..5750)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5733..5749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5742..5764)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5891..6346
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5978..5997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6188..6209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6372..6476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6477..6545,6546..7337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6695..6714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7508..8096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7997..8016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8250..8267
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8384..8405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8420..8425,8426..8443,8444..8450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8458..8474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8463..8485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8482..8498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8482..8498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8517..8537
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8519..8537
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8565..8791
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8792..8972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9019..9144
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9637..9870
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10055..10099
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10274..10774
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10693..10711
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10786..11181
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11241..11358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
       61 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      121 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      181 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      241 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      301 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      361 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      421 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      481 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      541 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
      601 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      661 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      721 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      781 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      841 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      901 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      961 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1021 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1081 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1141 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1201 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1261 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     1321 tctcgagctc aagcttacat gtggtgcctg cactgcaact cggagaggac ccagtccctt
     1381 ctggagctag agcttgacag cggcgtcgag ggcgaggccc ccagcagtga gactggcaca
     1441 tccctggaca gcccctcagc ctacccccag ggccccttgg tgcccggttc cagcctgagc
     1501 ccggatcact acgagcacac gtcagtggga gcctatgggc tgtactcggg gccgccgggg
     1561 caacagcagc gcacgcggag gcccaagctg cagcactcga cctccatcct gcgcaagcag
     1621 gctgaggagg aggccatcaa gcgctcacgc tcactctccg agagctatga gctctcctcg
     1681 gacctgcagg acaagcaggt ggagatgcta gagcgaaagt atggggggcg cctggtaacc
     1741 cgccatgcgg cccgcaccat ccagacggcg tttcgccaat atcaaatgaa taaaaacttc
     1801 gagcgcttgc gcagctccat gtcagagaac cgcatgtcac gccggattgt gctgtccaac
     1861 atgaggatgc agttctcctt tgaggggcct gagaaagtgc acagctccta cttcgagggg
     1921 aagcaggtct cagtgactaa cgacggctcc cagctgggag ccctggtgtc ccctgagtgt
     1981 ggtgacctca gcgagcccac caccctcaag tctccggccc cctccagtga ctttgcggac
     2041 gccatcaccg agctggagga cgccttctct aggcaagtga aatcactggc cgagtccatc
     2101 gacgatgccc tcaactgccg cagcctgcac actgaggagg caccggccct ggatgcggcg
     2161 cgggcccggg acaccgaacc ccagacagcc ctgcacggca tggaccaccg caaactggac
     2221 gagatgacgg cctcgtacag tgatgtcacc ctgtacatcg atgaggagga gctgtcgccc
     2281 cctctgcccc tctcgcaggc aggggaccgg ccgtccagca ccgagtcgga cctgcggcta
     2341 cgggctgggg gcgcagcccc agactactgg gccctggccc acaaagagga caaggctgac
     2401 acggacacga gctgccggag cacgccgtcg ctggagcggc aggagcagcg gctgcgggtg
     2461 gagcatctgc cgctgctcac catcgagcca cccagcgaca gctctgtgga ccttagtgac
     2521 cgctcggagc gggggtcact caagaggcag agtgcttacg agcgcagcct tggcgggcag
     2581 cagggcagtc ccaagcatgg tccccacagc ggcgccccca agagcctccc ccgggaggag
     2641 cctgagttgc ggccccggcc ccccaggccc ctggacagcc acttggccat caatggctca
     2701 gccaaccggc agagcaagtc tgagtcggac tactcagacg gtgacaatga cagcatcaac
     2761 agcacgtcca actccaacga taccatcaac tgcagctccg agtcatcgtc ccgtgacagc
     2821 ctgcgggagc agacgctcag caagcagacc taccacaagg aggcccgcaa cagctgggac
     2881 tcgcctgcct ttagcaacga tgtcatccgc aagaggcact accgcatcgg cctgaacctc
     2941 ttcaacaaga agcctgagaa gggagtccag tacctcatcg agcgtggctt tgtgcccgac
     3001 acgcccgtcg gggtggccca cttcctgctg cagcgcaagg gcctcagccg gcagatgatc
     3061 ggcgagttcc tgggcaaccg gcagaagcag ttcaaccgtg acgtgctcga ctgcgtcgtg
     3121 gacgagatgg acttctctac catggagctg gatgaggccc tcaggaaatt ccaggcgcac
     3181 atccgtgtcc aaggggaggc tcagaaagtg gagcggctca tagaggcgtt cagccagcgc
     3241 tactgcatct gcaaccctgg ggtggtgcgg caattccgga acccagacac cattttcatc
     3301 ctggccttcg ccatcatcct gctgaacacc gacatgtaca gccccaatgt caagcccgag
     3361 cggaaaatga agctagagga cttcatcaag aacctccgag gtgtggacga tggtgaggac
     3421 attccccgtg agatgctgat ggggatctat gaacggatcc gtaagcgaga gctaaagacc
     3481 aatgaggacc atgtgtccca ggtgcagaag gtggagaagc tcattgtggg gaaaaagccg
     3541 atcggatccc tgcatcccgg gctcggctgt gtgctctctc tgccccaccg tcggttggtc
     3601 tgctactgcc ggctctttga ggttccagac ccaaacaagc cccagaaact cggactacac
     3661 cagcgagaaa tcttcctgtt caacgacctc ctggtggtca ccaagatctt ccagaagaag
     3721 aagaactcgg tgacgtacag cttccgacag tccttctcct tgtacggcat gcaggtcctg
     3781 ctcttcgaga accagtacta ccccaatggc atccggctca cctcgtctgt ccccggagca
     3841 gatatcaaag tgttaataaa cttcaacgcc cccaaccctc aagaccggaa gaaattcacc
     3901 gatgacctgc gggagtccat tgcggaagtc caagagatgg agaagcacag gatagagtcg
     3961 gagctcgaga agcagaaagg cgtcgtgcgg cccagcatgt cccagtgctc tagcctcaaa
     4021 aaggagtcgg gcaacggaac actgagccgg gcctgcctgg acgacagcta tgccagcggt
     4081 gagggcctca agcgcagcgc cctcagcagc tccctgcggg acctctcgga agccgggaag
     4141 cgagggcgtc gcagcagtgc gggatcgcta gagagcaatg tggaatttca gcctttcgag
     4201 ccactgcagc cgtcagtgct gtgctcctaa gaattctgca gtcgacggta ccgcgggccc
     4261 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     4321 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4381 tgttgttgtt gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     4441 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4501 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4561 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4621 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4681 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4741 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4801 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4861 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4921 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4981 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     5041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5281 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     5341 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5521 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5581 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5641 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     5701 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     5761 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5821 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5881 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6301 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8221 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8281 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8461 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     8521 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     8581 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     8641 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     8701 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     8761 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     8821 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8881 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8941 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9001 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9061 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9121 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9181 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9241 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9301 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9361 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9421 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     9481 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     9541 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     9601 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     9661 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9721 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9781 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9841 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9901 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9961 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    10021 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    10081 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    10141 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    10201 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    10261 cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
    10321 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
    10381 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
    10441 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
    10501 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
    10561 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
    10621 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
    10681 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
    10741 cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg
    10801 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
    10861 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
    10921 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
    10981 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
    11041 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
    11101 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
    11161 tctggttatg tgtgggaggg cctgcagctg cagtagtaag aattctagat cttgagacaa
    11221 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    11281 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    11341 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
    11401 ctcgaggggg cccggggtta ttaatagtaa tcaat
//
