LOCUS       sequence-318831-        8950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of tagged ARF WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-ARF5-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    248..481
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             666..710
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        885..1385
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1304..1322
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1397..1792
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1852..1969
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2086..2389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2390..2593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2544..2564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3217..3226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3223..3933
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      3478..3487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3978..4566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4031..4051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4449..4460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4638..4871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4949..5070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4986..5005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5040..5059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5110..5245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5265..5284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5266..5284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5294..5311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5294..5310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5303..5325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5452..5907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5749..5770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7811..7828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7945..7966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7981..7986,7987..8004,8005..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8024..8046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8043..8059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8043..8059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8078..8098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8080..8098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8126..8352
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8353..8533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8580..8705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
       61 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
      121 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      181 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      241 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      301 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      361 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      421 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      481 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      541 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      601 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      661 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      721 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
      781 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
      841 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
      901 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
      961 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     1021 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     1081 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     1141 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     1201 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     1261 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     1321 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     1381 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     1441 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     1501 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     1561 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     1621 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     1681 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     1741 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     1801 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     1861 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1921 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     1981 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     2041 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2101 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2161 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2221 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2281 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2341 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2401 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2461 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2521 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2581 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
     2641 gagaccatgg gcctcaccgt gtccgcgctc ttttcgcgga tcttcgggaa gaagcagatg
     2701 cggattctca tggttggctt ggatgcggct ggcaagacca caatcctgta caaactgaag
     2761 ttgggggaga ttgtcaccac catcccaacc ataggcttca atgtagaaac agtggaatat
     2821 aagaacatct gtttcacagt ctgggacgtg ggaggccagg acaagattcg gcctctgtgg
     2881 cggcactact tccagaacac tcagggcctc atctttgtgg tggacagtaa tgaccgggag
     2941 cgggtccaag aatctgctga tgaactccag aagatgctgc aggaggacga gctgcgggac
     3001 gccgtattgc tggtcttcgc caacaagcag gacatgccca acgccatgcc cgtgagcgag
     3061 ctgactgaca agctggggct acagcactta cgcagccgca cgtggtatgt ccaggccacc
     3121 tgtgccaccc aaggcacagg tctgtacgat ggtctggact ggctgtccca cgagctgtca
     3181 aagcgcgcgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
     3241 gaggataaca tggcctctct cccagcgaca catgagttac acatctttgg ctccatcaac
     3301 ggtgtggact ttgacatggt gggtcagggc accggcaatc caaatgatgg ttatgaggag
     3361 ttaaacctga agtccaccaa gggtgacctc cagttctccc cctggattct ggtccctcat
     3421 atcgggtatg gcttccatca gtacctgccc taccctgacg ggatgtcgcc tttccaggcc
     3481 gccatggtag atggctccgg ctaccaagtc catcgcacaa tgcagtttga agatggtgcc
     3541 tcccttactg ttaactaccg ctacacctac gagggaagcc acatcaaagg agaggcccag
     3601 gtgaagggga ctggtttccc tgctgacggt cctgtgatga ccaactcgct gaccgctgcg
     3661 gactggtgca ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag tacctttaag
     3721 tggagttaca ccactggaaa tggcaagcgc taccggagca ctgcgcggac cacctacacc
     3781 tttgccaagc caatggcggc taactatctg aagaaccagc cgatgtacgt gttccgtaag
     3841 acggagctca agcactccaa gaccgagctc aacttcaagg agtggcaaaa ggcctttacc
     3901 gatgtgatgg gcatggacga gctgtacaag taaagcggcc gcgactctag cgctaaccgg
     3961 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4381 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4561 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4681 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4741 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4801 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4861 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     4921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     4981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5221 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     5461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5881 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7861 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7921 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7981 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8041 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8101 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8161 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8221 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8281 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8341 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8581 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8641 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8701 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8761 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8821 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8881 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8941 ccctctattg
//
