LOCUS       sequence-318833-        9136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of tagged ARF WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX301-ARF6-TagRFP
FEATURES             Location/Qualifiers
     source          1..9136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        129..432
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        433..636
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     587..607
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1195..1204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1201..1932
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    2011..2599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2064..2084)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2482..2493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2671..2904
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2982..3103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3019..3038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3073..3092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3143..3278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3298..3317)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3299..3317)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3327..3344)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3327..3343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3336..3358)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3485..3940
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3572..3591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3782..3803
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3966..4070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4071..4139,4140..4931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4289..4308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5102..5690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5591..5610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5844..5861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5978..5999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6014..6019,6020..6037,6038..6044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6052..6068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6057..6079
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6076..6092
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6076..6092
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6111..6131
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6113..6131
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6159..6385
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6386..6566
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6613..6738
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7231..7464
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7649..7693
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7868..8368
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8287..8305
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8390..8989
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8390..8409)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8886..8906
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(9031..9136,1..12)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
       61 ggggcccggg gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      121 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      181 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      241 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      301 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      361 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      421 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      481 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      541 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      601 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
      661 tagatgggga aggtgctatc caaaatcttc gggaacaagg aaatgcggat cctcatgttg
      721 ggcctggacg cggccggcaa gacaacaatc ctgtacaagt tgaagctggg ccagtcggtg
      781 accaccattc ccactgtggg tttcaacgtg gagacggtga cttacaaaaa tgtcaagttc
      841 aacgtatggg atgtgggcgg ccaggacaag atccggccgc tctggcggca ttactacact
      901 gggacccaag gtctcatctt cgtagtggac tgcgccgacc gcgaccgcat cgatgaggct
      961 cgccaggagc tgcaccgcat tatcaatgac cgggagatga gggacgccat aatcctcatc
     1021 ttcgccaaca agcaggacct gcccgatgcc atgaaacccc acgagatcca ggagaaactg
     1081 ggcctgaccc ggattcggga caggaactgg tatgtgcagc cctcctgtgc cacctcaggg
     1141 gacggactct atgagggcct gacctggctg accagcaact acaaatctcc ggtcgccacc
     1201 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
     1261 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     1321 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     1381 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
     1441 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     1501 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1561 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     1621 acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
     1681 agaaccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc
     1741 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     1801 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
     1861 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
     1921 gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
     1981 accgcgggcc cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2401 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2521 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2581 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     2641 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2701 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2761 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2821 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2881 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     2941 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3121 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3181 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3241 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3301 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3361 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3421 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3481 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3541 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3601 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3661 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3721 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3781 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3841 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3901 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4021 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4081 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4141 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4201 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4261 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4321 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4381 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4441 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4501 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4561 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4621 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4681 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5221 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5461 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6001 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6061 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6121 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6181 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6241 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6301 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6361 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6541 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6601 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6661 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6721 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6781 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6841 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6901 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6961 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7021 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7081 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7141 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7201 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7261 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7321 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7381 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7441 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7501 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7561 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7621 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7681 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7741 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7801 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7861 gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     7921 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     7981 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     8041 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     8101 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     8161 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     8221 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     8281 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     8341 tgaccgaatc accgacctct ctccccaggg ggtaccaccg gagcttacca tgaccgagta
     8401 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8461 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8521 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8581 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     8641 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8701 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8761 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8821 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8881 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8941 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag aattctagat
     9001 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9061 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9121 aaaacaaatt acaaaa
//
