LOCUS       sequence-318835-        8512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retrovirus construct for stable expressing mEGFP tagged NDP52.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris
            DP, Fogel AI, Youle RJ
  TITLE     The ubiquitin kinase PINK1 recruits autophagy receptors to induce 
            mitophagy.
  JOURNAL   Nature. 2015 Aug 20;524(7565):309-14. doi: 10.1038/nature14893. Epub
            2015 Aug 12.
  PUBMED    26266977
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14893"; journalName: "Nature"; date: "2015-08-20- 
            20"; volume: "524"; issue: "7565"; pages: "309-14"
COMMENT     Sequence Label: pBMN-2xFKBP-mEGFP-NDP52
FEATURES             Location/Qualifiers
     source          1..8512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1765..1767,1768..2088)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2095..2415
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2095..2415
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(2422..2424,2425..3135)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2464..2485)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2725..2744)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3072..3093
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4712..5181
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5311..5328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5482..6070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5562..5581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6234..7025,7026..7094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6857..6876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7095..7199)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  7344..8111
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg ccacatggga gtgcaggtgg aaaccatctc cccaggagac
     1801 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
     1861 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
     1921 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
     1981 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
     2041 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagaggcgtg
     2101 caggtcgaga ccatcagccc cggcgacggc cgcacctttc ccaagagagg ccagacttgc
     2161 gtggtccact acaccggcat gctggaggac ggcaagaagt tcgacagcag ccgcgaccgc
     2221 aacaagccct tcaagttcat gctgggcaaa caggaagtga tccgcggctg ggaggaaggc
     2281 gtggctcaga tgagcgtggg gcagcgggcc aagctgacca tcagccccga ctatgcctac
     2341 ggcgccaccg gccaccccgg catcatcccc ccccacgcca ccctggtgtt cgacgtggag
     2401 ctgctgaagc tggagactag tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2461 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2521 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2581 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2641 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2701 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2761 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2821 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2881 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2941 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3001 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     3061 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3121 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
     3181 gaggagacca tcaaagatcc ccccacatca gctgtcttgc tggatcactg tcatttctct
     3241 caggtcatct ttaacagtgt ggagaagttc tacatccctg gaggggacgt cacatgtcat
     3301 tataccttca cccagcattt catccctcgt cgaaaggatt ggattggcat ctttagagtg
     3361 gggtggaaga caacccgtga gtattacacc ttcatgtggg ttactttgcc cattgaccta
     3421 aacaacaaat cagctaaaca gcaggaagtc caattcaaag cttactacct gcccaaggat
     3481 gatgagtatt accagttctg ctatgtggat gaggatggtg tggtccgggg agcaagtatt
     3541 cctttccaat tccgtccaga aaatgaggaa gacatcctgg ttgttaccac tcagggagag
     3601 gtggaagaga ttgagcagca caacaaggag ctttgcaaag aaaaccagga gctgaaggac
     3661 agctgtatca gcctccagaa gcagaactca gacatgcagg ctgagctcca aaagaagcag
     3721 gaggagctag aaaccctaca gagcatcaat aagaagttgg aactgaaagt gaaagaacag
     3781 aaggactatt gggagacaga gctgcttcaa ctgaaagaac aaaaccagaa gatgtcctca
     3841 gaaaatgaga agatgggaat cagagtggat cagcttcagg cccagctgtc aactcaagag
     3901 aaagaaatgg agaagcttgt tcagggagat caagataaga cagagcagtt agagcagctg
     3961 aaaaaggaaa atgaccacct ctttctcagt ttagctgaac agaggaagga ccagaagaag
     4021 ctcgagcaga cagtggagca aatgaagcag aatgaaacta ctgcaatgaa gaaacaacag
     4081 gaattaatgg atgaaaactt tgacctgtca aaaagactga gtgagaacga aattatatgt
     4141 aatgctctgc agagacagaa agagagattg gaaggagaaa atgatctttt gaagagggag
     4201 aacagcagat tgctcagtta catgggtctg gattttaatt ctttgccgta tcaagtacct
     4261 acttcagatg aaggaggcgc aagacaaaat ccaggacttg cctatggaaa cccatattct
     4321 ggtatccaag aaagttcttc ccccagcccg ctctccatca agaaatgccc tatctgcaaa
     4381 gcagatgata tttgtgatca caccttggag caacagcaga tgcagcccct ttgtttcaat
     4441 tgtccaattt gtgacaagat cttcccagct acagagaagc agatctttga agaccacgtg
     4501 ttctgccact ctctctgagg atccaccgga tctagataac tgatcacgat aaaataaaag
     4561 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     4621 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     4681 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4741 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     4801 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     4861 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     4921 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     4981 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     5041 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     5101 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     5161 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     5221 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg
     5281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6181 gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6241 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6301 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6361 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6421 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6481 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6541 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6601 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6661 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6721 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6781 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6841 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6901 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6961 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7021 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7081 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattat
     7141 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7201 acatttcccc gaaaagtgcc accagctttg ctcttaggag tttcctaata catcccaaac
     7261 tcaaatatat aaagcatttg acttgttcta tgccctaggg ggcgggggga agctaagcca
     7321 gcttttttta acatttaaaa tgtggtaatg actccaactt attgatagtg ttttatgttc
     7381 agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc
     7441 cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta
     7501 tatcgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca
     7561 tccgtcatcc atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc
     7621 gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg
     7681 taaaacagcc agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc
     7741 gcgcagacga tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt
     7801 tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc
     7861 atccaacgcc attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg
     7921 tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc
     7981 cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga
     8041 tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg
     8101 gcagcatcac cttaaaatgt taattccatt ttaaatgcac agatgttttt atttcataag
     8161 ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca aagctagtat aaataaaaat
     8221 agataaacgt ggaaattact tagagtttct gtcattaacg tttccttcct cagttgacaa
     8281 cataaatgcg ctgctgagaa gccagtttgc atctgtcagg atcaatttcc cattatgcca
     8341 gtcatattaa ttactagtca attagttgat ttttattttt gacatataca tgtgaaagac
     8401 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     8461 acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac ag
//
