LOCUS       sequence-318857-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of tagged ARF WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX303-ARF6-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     537..557
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      615..624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1161..1170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1167..1877
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1422..1431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1922..2510
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1975..1995)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2393..2404)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2582..2815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2893..3014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2930..2949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2984..3003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3054..3189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3209..3228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3210..3228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3238..3255)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3238..3254)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3247..3269)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3396..3851
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3483..3502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3693..3714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3877..3981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3982..4050,4051..4842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4200..4219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5013..5601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5502..5521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5755..5772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5889..5910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5925..5930,5931..5948,5949..5955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5963..5979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5968..5990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5987..6003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5987..6003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6022..6042
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6024..6042
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6070..6296
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6297..6477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6524..6649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7142..7375
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7560..7604
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7779..8279
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8198..8216
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8291..8686
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8746..8863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      421 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      481 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      601 tcagatccgc tagcgccacc atggggaagg tgctatccaa aatcttcggg aacaaggaaa
      661 tgcggatcct catgttgggc ctggacgcgg ccggcaagac aacaatcctg tacaagttga
      721 agctgggcca gtcggtgacc accattccca ctgtgggttt caacgtggag acggtgactt
      781 acaaaaatgt caagttcaac gtatgggatg tgggcggcca ggacaagatc cggccgctct
      841 ggcggcatta ctacactggg acccaaggtc tcatcttcgt agtggactgc gccgaccgcg
      901 accgcatcga tgaggctcgc caggagctgc accgcattat caatgaccgg gagatgaggg
      961 acgccataat cctcatcttc gccaacaagc aggacctgcc cgatgccatg aaaccccacg
     1021 agatccagga gaaactgggc ctgacccgga ttcgggacag gaactggtat gtgcagccct
     1081 cctgtgccac ctcaggggac ggactctatg agggcctgac ctggctgacc agcaactaca
     1141 aatctgagga tccaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcct
     1201 ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca
     1261 tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca
     1321 ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc
     1381 atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct
     1441 ccggctacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact
     1501 accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt
     1561 tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga
     1621 agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg
     1681 gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc aagccaatgg
     1741 cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact
     1801 ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg
     1861 acgagctgta caagtaaagc ggccgcgact ctagcgctaa ccggtggcgc gttaagtcga
     1921 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1981 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2041 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2101 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2161 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2221 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2281 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2341 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2401 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2461 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     2521 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     2581 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     2641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     2761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     2821 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     2881 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3001 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     3061 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3121 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3181 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     3241 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3301 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3361 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3421 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3481 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3541 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3601 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3661 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3721 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3781 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3841 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4201 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4501 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5701 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5761 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5821 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5881 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5941 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6001 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6061 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6121 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6181 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6241 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     6481 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     6541 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     6601 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     6661 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     6721 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     6781 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     6841 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     6901 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     6961 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7021 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7081 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7141 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7201 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     7261 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     7321 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     7381 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     7441 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     7501 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     7561 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     7621 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     7681 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     7741 tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc
     7801 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     7861 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     7921 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     7981 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     8041 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     8101 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     8161 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     8221 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     8281 gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     8341 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     8401 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     8461 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     8521 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     8581 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     8641 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta
     8701 gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     8761 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     8821 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     8881 cagcagagat ccactttggc g
//
