LOCUS       sequence-318858-        8431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tagged vector control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    400..633
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             818..862
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1037..1537
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1456..1474
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1549..1944
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2004..2121
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2238..2541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2542..2745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2696..2716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2768..2848
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2850..2859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2856..3566
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      3111..3120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3611..4199
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3664..3684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4082..4093)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4271..4504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4582..4703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4619..4638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4673..4692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4743..4878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4898..4917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4899..4917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4927..4944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4927..4943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4936..4958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5085..5540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5172..5191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5382..5403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5566..5670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5671..5739,5740..6531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5889..5908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6702..7290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7191..7210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7444..7461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7578..7599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7614..7619,7620..7637,7638..7644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7652..7668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7657..7679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7676..7692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7676..7692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7711..7731
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7713..7731
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7759..7985
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7986..8166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8213..8338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
       61 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
      121 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
      181 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
      241 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
      301 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
      361 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
      421 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
      481 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
      541 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
      601 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
      661 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
      721 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
      781 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
      841 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
      901 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
      961 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1021 aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     1081 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     1141 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     1201 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     1261 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     1321 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     1381 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     1441 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     1501 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct
     1561 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     1621 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     1681 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     1741 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     1801 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     1861 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     1921 ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga
     1981 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2041 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2101 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     2161 cggctcgagg gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc
     2221 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2281 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2341 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2401 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2461 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2521 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2581 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2641 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2701 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2761 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     2821 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata
     2881 acatggcctc tctcccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg
     2941 actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc
     3001 tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt
     3061 atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg
     3121 tagatggctc cggctaccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta
     3181 ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg
     3241 ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt
     3301 gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt
     3361 acaccactgg aaatggcaag cgctaccgga gcactgcgcg gaccacctac acctttgcca
     3421 agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc
     3481 tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga
     3541 tgggcatgga cgagctgtac aagtaaagcg gccgcgactc tagcgctaac cggtggcgcg
     3601 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3661 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3721 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3781 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3841 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3901 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3961 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4021 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4081 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4141 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4201 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4261 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4501 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4561 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4681 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4921 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4981 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5041 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5101 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5161 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5221 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5281 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5341 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5401 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5461 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5521 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5881 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6361 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6841 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7321 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7381 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7441 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7501 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7561 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7621 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7681 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7741 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7801 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7861 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7921 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7981 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8161 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8221 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     8281 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8341 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8401 gaaaaaatat aaattaaaac atatagtatg g
//
