LOCUS       sequence-318864-        8542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tagged vector control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-GFP
FEATURES             Location/Qualifiers
     source          1..8542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             471..515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        690..1190
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1109..1127
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1202..1597
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1657..1774
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1891..2194
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2195..2398
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2349..2369
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2437..2446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2443..2445,2446..2448,2449..3159)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2443..2445,2446..2448,2449..3159)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2488..2509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2749..2768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3096..3117
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3160..3225
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3375..3963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3428..3448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3846..3857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4035..4268
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4346..4467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4383..4402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4437..4456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4507..4642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4662..4681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4663..4681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4691..4708)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4691..4707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4700..4722)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4849..5304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4936..4955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5146..5167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5330..5434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5435..5503,5504..6295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5653..5672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6466..7054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6955..6974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7208..7225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7342..7363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7378..7383,7384..7401,7402..7408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7416..7432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7421..7443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7440..7456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7440..7456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7475..7495
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7477..7495
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7523..7749
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7750..7930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7977..8102
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
       61 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
      121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
      181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
      241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
      301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
      361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
      421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
      481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
      541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
      601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
      661 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
      721 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
      781 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
      841 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
      901 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
      961 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     1021 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     1081 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     1141 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     1201 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     1261 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     1321 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     1381 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     1441 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     1501 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     1561 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     1621 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     1681 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     1741 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     1801 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     1861 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1921 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1981 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2041 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2101 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2161 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2221 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2281 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2341 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2401 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     2461 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2521 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2581 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     2641 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     2701 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2761 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2821 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2881 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2941 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3001 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3061 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3121 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct
     3181 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa
     3241 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3301 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttgc taaccggtgg
     3361 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3421 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3481 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3541 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3601 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3661 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3721 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3781 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3841 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3901 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3961 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4021 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4081 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4141 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4201 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4261 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     4321 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     4381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4441 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     4501 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4561 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4621 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     4681 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4801 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     4861 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4921 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4981 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5041 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5101 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5161 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5221 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5281 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6121 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7261 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     7321 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7381 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7441 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     7501 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     7561 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     7621 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     7681 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     7741 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7801 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7861 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7921 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7981 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     8041 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     8101 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     8161 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8221 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8281 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8341 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8401 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     8461 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     8521 attgaaccat taggagtagc ac
//
