LOCUS       sequence-318866-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast cycling ARF mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX303-ARF6-T157N-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        364..567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     518..538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      596..605
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1148..1858
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1403..1412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1903..2491
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1956..1976)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2374..2385)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2563..2796
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2874..2995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2911..2930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2965..2984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3035..3170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3190..3209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3191..3209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3219..3236)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3219..3235)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3228..3250)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3377..3832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3464..3483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3674..3695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3858..3962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3963..4031,4032..4823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4181..4200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4994..5582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5483..5502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5736..5753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5870..5891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5906..5911,5912..5929,5930..5936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5944..5960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5949..5971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5968..5984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5968..5984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6003..6023
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6005..6023
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6051..6277
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6278..6458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6505..6630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7123..7356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7541..7585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7760..8260
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8179..8197
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8272..8667
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8727..8844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgccac
      601 catggggaag gtgctatcca aaatcttcgg gaacaaggaa atgcggatcc tcatgttggg
      661 cctggacgcg gccggcaaga caacaatcct gtacaagttg aagctgggcc agtcggtgac
      721 caccattccc actgtgggtt tcaacgtgga gacggtgact tacaaaaatg tcaagttcaa
      781 cgtatgggat gtgggcggcc aggacaagat ccggccgctc tggcggcatt actacactgg
      841 gacccaaggt ctcatcttcg tagtggactg cgccgaccgc gaccgcatcg atgaggctcg
      901 ccaggagctg caccgcatta tcaatgaccg ggagatgagg gacgccataa tcctcatctt
      961 cgccaacaag caggacctgc ccgatgccat gaaaccccac gagatccagg agaaactggg
     1021 cctgacccgg attcgggaca ggaactggta tgtgcagccc tcctgtgcca actcagggga
     1081 cggactctat gagggcctga cctggctgac cagcaactac aaatctgagg atccaccggt
     1141 cgccaccatg gtgagcaagg gcgaggagga taacatggcc tctctcccag cgacacatga
     1201 gttacacatc tttggctcca tcaacggtgt ggactttgac atggtgggtc agggcaccgg
     1261 caatccaaat gatggttatg aggagttaaa cctgaagtcc accaagggtg acctccagtt
     1321 ctccccctgg attctggtcc ctcatatcgg gtatggcttc catcagtacc tgccctaccc
     1381 tgacgggatg tcgcctttcc aggccgccat ggtagatggc tccggctacc aagtccatcg
     1441 cacaatgcag tttgaagatg gtgcctccct tactgttaac taccgctaca cctacgaggg
     1501 aagccacatc aaaggagagg cccaggtgaa ggggactggt ttccctgctg acggtcctgt
     1561 gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg aagaagactt accccaacga
     1621 caaaaccatc atcagtacct ttaagtggag ttacaccact ggaaatggca agcgctaccg
     1681 gagcactgcg cggaccacct acacctttgc caagccaatg gcggctaact atctgaagaa
     1741 ccagccgatg tacgtgttcc gtaagacgga gctcaagcac tccaagaccg agctcaactt
     1801 caaggagtgg caaaaggcct ttaccgatgt gatgggcatg gacgagctgt acaagtaaag
     1861 cggccgcgac tctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggattac
     1921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2281 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2461 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     2521 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     2581 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     2641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     2821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     2881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3001 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3061 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3121 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3361 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3421 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3481 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3541 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3601 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3661 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3721 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3781 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6001 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6061 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6121 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6181 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6241 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6301 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6361 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6421 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6481 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6541 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6601 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6661 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6721 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6781 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6841 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6901 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6961 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7021 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7081 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7141 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7201 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7261 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7321 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7381 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7441 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7501 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7561 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7621 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7681 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7741 cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     7801 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     7861 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     7921 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     7981 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     8041 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     8101 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     8161 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     8221 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac catggccaag
     8281 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     8341 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     8401 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     8461 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     8521 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     8581 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     8641 ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt ctagatcttg
     8701 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     8761 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     8821 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     8881 cgccggctcg agggggcccg g
//
