LOCUS       sequence-318867-       11888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 splice variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-GFP-v1 IQSEC1 WT
FEATURES             Location/Qualifiers
     source          1..11888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..339
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        340..543
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     494..514
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      582..591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(588..590,591..593,594..1304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(588..590,591..593,594..1304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(633..654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(894..913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1241..1262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4455..4475
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    4866..5454
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4919..4939)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5337..5348)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5526..5759
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5837..5958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5874..5893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5928..5947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5998..6133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6153..6172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6154..6172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6182..6199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6182..6198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6191..6213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6340..6795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6427..6446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6637..6658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6821..6925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6926..6994,6995..7786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7144..7163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7957..8545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8446..8465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8699..8716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8833..8854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8869..8874,8875..8892,8893..8899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8907..8923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8912..8934
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8931..8947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8931..8947
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8966..8986
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8968..8986
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9014..9240
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9241..9421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9468..9593
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10086..10319
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10504..10548
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10723..11223
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     11142..11160
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             11235..11630
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11690..11807
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
       61 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      121 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      181 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      241 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      301 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      361 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      421 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      481 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      541 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
      601 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      661 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      721 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      781 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      841 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      901 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      961 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1021 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1081 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1141 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1201 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1261 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     1321 gagctcaagc ttacatggct tgcagaagac gctatttcgt cgagggcgag gcccccagca
     1381 gtgagactgg cacatccctg gacagcccct cagcctaccc ccagggcccc ttggtgcccg
     1441 gttccagcct gagcccggat cactacgagc acacgtcagt gggagcctat gggctgtact
     1501 cggggccgcc ggggcaacag cagcgcacgc ggaggcccaa gctgcagcac tcgacctcca
     1561 tcctgcgcaa gcaggctgag gaggaggcca tcaagcgctc acgctcactc tccgagagct
     1621 atgagctctc ctcggacctg caggacaagc aggtggagat gctagagcga aagtatgggg
     1681 ggcgcctggt aacccgccat gcggcccgca ccatccagac ggcgtttcgc caatatcaaa
     1741 tgaataaaaa cttcgagcgc ttgcgcagct ccatgtcaga gaaccgcatg tcacgccgga
     1801 ttgtgctgtc caacatgagg atgcagttct cctttgaggg gcctgagaaa gtgcacagct
     1861 cctacttcga ggggaagcag gtctcagtga ctaacgacgg ctcccagctg ggagccctgg
     1921 tgtcccctga gtgtggtgac ctcagcgagc ccaccaccct caagtctccg gccccctcca
     1981 gtgactttgc ggacgccatc accgagctgg aggacgcctt ctctaggcaa gtgaaatcac
     2041 tggccgagtc catcgacgat gccctcaact gccgcagcct gcacactgag gaggcaccgg
     2101 ccctggatgc ggcgcgggcc cgggacaccg aaccccagac agccctgcac ggcatggacc
     2161 accgcaaact ggacgagatg acggcctcgt acagtgatgt caccctgtac atcgatgagg
     2221 aggagctgtc gccccctctg cccctctcgc aggcagggga ccggccgtcc agcaccgagt
     2281 cggacctgcg gctacgggct gggggcgcag ccccagacta ctgggccctg gcccacaaag
     2341 aggacaaggc tgacacggac acgagctgcc ggagcacgcc gtcgctggag cggcaggagc
     2401 agcggctgcg ggtggagcat ctgccgctgc tcaccatcga gccacccagc gacagctctg
     2461 tggaccttag tgaccgctcg gagcgggggt cactcaagag gcagagtgct tacgagcgca
     2521 gccttggcgg gcagcagggc agtcccaagc atggtcccca cagcggcgcc cccaagagcc
     2581 tcccccggga ggagcctgag ttgcggcccc ggccccccag gcccctggac agccacttgg
     2641 ccatcaatgg ctcagccaac cggcagagca agtctgagtc ggactactca gacggtgaca
     2701 atgacagcat caacagcacg tccaactcca acgataccat caactgcagc tccgagtcat
     2761 cgtcccgtga cagcctgcgg gagcagacgc tcagcaagca gacctaccac aaggaggccc
     2821 gcaacagctg ggactcgcct gcctttagca acgatgtcat ccgcaagagg cactaccgca
     2881 tcggcctgaa cctcttcaac aagaagcctg agaagggagt ccagtacctc atcgagcgtg
     2941 gctttgtgcc cgacacgccc gtcggggtgg cccacttcct gctgcagcgc aagggcctca
     3001 gccggcagat gatcggcgag ttcctgggca accggcagaa gcagttcaac cgtgacgtgc
     3061 tcgactgcgt cgtggacgag atggacttct ctaccatgga gctggatgag gccctcagga
     3121 aattccaggc gcacatccgt gtccaagggg aggctcagaa agtggagcgg ctcatagagg
     3181 cgttcagcca gcgctactgc atctgcaacc ctggggtggt gcggcaattc cggaacccag
     3241 acaccatttt catcctggcc ttcgccatca tcctgctgaa caccgacatg tacagcccca
     3301 atgtcaagcc cgagcggaaa atgaagctag aggacttcat caagaacctc cgaggtgtgg
     3361 acgatggtga ggacattccc cgtgagatgc tgatggggat ctatgaacgg atccgtaagc
     3421 gagagctaaa gaccaatgag gaccatgtgt cccaggtgca gaaggtggag aagctcattg
     3481 tggggaaaaa gccgatcgga tccctgcatc ccgggctcgg ctgtgtgctc tctctgcccc
     3541 accgtcggtt ggtctgctac tgccggctct ttgaggttcc agacccaaac aagccccaga
     3601 aactcggact acaccagcga gaaatcttcc tgttcaacga cctcctggtg gtcaccaaga
     3661 tcttccagaa gaagaagaac tcggtgacgt acagcttccg acagtccttc tccttgtacg
     3721 gcatgcaggt cctgctcttc gagaaccagt actaccccaa tggcatccgg ctcacctcgt
     3781 ctgtccccgg agcagatatc aaggtgctga tcaacttcaa cgcccccaac ccccaggacc
     3841 ggaagaagtt caccgacgac ctgcgcgagt ctatcgccga ggtgcaggaa atggaaaagc
     3901 accggatcga gagcgagctg gaaaagcaga aaggcgtggt gcgccccagc atgagccagt
     3961 gcagcagcct gaagaaagag agcggcaacg gcaccctgag ccgggcctgc ctggatgata
     4021 gctacgccag cggcgagggc ctgaagagaa gcgccctgag cagctccctg cgggatctga
     4081 gcgaggccgg caagcggggc agaagaagca gcgccggcag cctggaaagc aacgtggaag
     4141 gcagcatcat cagcagcccc cacatgcggc ggagagccac cagcaccaga gagtgcccca
     4201 gcagacccca ccagaccatg cccaactcct ccagcctgct gggcagcctg ttcggcagca
     4261 agagaggcaa gccaccaccc caggcccatc tgccatctgc ccctgccctg cctcctccac
     4321 accctcccgt ggtgctgcct catctgcagc acagcgtggc cggacaccac ctgggacctc
     4381 cagaaggact gccccaggcc gccatgcacg gccatcacac ccagtactgc cacatgcaga
     4441 acccccctcc ctaccaccac catcaccatc atcacccccc ccagcacatc cagcacgccc
     4501 accagtatca ccacggccct cacggcggac accctgccta tggcgcccac gcccatggac
     4561 accctccact gcctagcgcc catgtgggac acacagtgca ccaccacgga cagcctcccg
     4621 cccctccacc tcctaccagc agcaaggcca agcccagcgg catcagcacc attgtgtgag
     4681 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata
     4741 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4801 ctgaacctga aacataaaat gaatgcaatt gttgttgttg ctaaccggtg gcgcgttaag
     4861 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4921 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4981 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5041 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5101 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5161 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5221 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5281 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5341 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5401 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5521 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5761 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5821 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5881 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5941 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6001 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6061 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6121 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6181 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6241 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6301 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6781 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6841 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6901 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6961 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7021 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7081 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7141 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7201 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7261 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7321 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7381 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7441 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7501 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7561 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7621 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7681 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7741 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7801 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7861 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7921 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7981 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8041 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8101 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8161 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8221 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8281 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8341 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8401 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8461 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8521 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8581 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8641 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8701 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8761 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8821 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8881 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8941 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9001 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     9061 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     9121 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     9181 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     9241 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9301 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9361 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9421 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9481 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9541 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9601 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9661 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9721 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9781 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9841 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9901 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     9961 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    10021 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    10081 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    10141 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    10201 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10261 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10321 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10381 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10441 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10501 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10561 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10621 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10681 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
    10741 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
    10801 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
    10861 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
    10921 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
    10981 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
    11041 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
    11101 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
    11161 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
    11221 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
    11281 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
    11341 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
    11401 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
    11461 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
    11521 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
    11581 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
    11641 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    11701 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    11761 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    11821 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
    11881 caattacg
//
