LOCUS       sequence-318895-        4398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the retron-TIR defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG005 (Retron-TIR)
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2264..2311
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2531..3190)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3105..3124
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3191..3293)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3685..3702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3819..4364)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3899..3918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
       61 agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
      121 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
      181 tgcgttgcgc aagttaatga ctattgtgag cgagaaacgc gctactacta tatatagaca
      241 gacaagatgc acttactgaa taaatactca taacggagaa accagctgta tagtgaacaa
      301 tagatttcca gtagcatatt tttacttcac ttttagttat taatatgata atcataaact
      361 acggctctgc cttaaatttg tgaggttgtt tcgcctcgaa ggaactaatg ttaggacata
      421 cgccaccgtt cagtcgatgg taacgcttct taactagtgg tccgctaagt gatgcgcaaa
      481 gtgattgggc agagccgaaa cgtttacaat ccgataggag ttggttttgt cgctacatga
      541 taaattatta atgcataact tcgcattagc caataaaaaa agccctgact tcatatctga
      601 acttcctcaa attgaaccta aaccatacag caatggacat aaaattaaat ggataaacca
      661 cacacttact agcactgaag ttactccccc tgataacctg attaaaatat gcatattgat
      721 tgagtcaggg gaaattgcta taacatcagt aagtgatatt gccaatttac ttggagttcc
      781 tgctggccaa ttactttata tactatatcg taaaaaagat aattatcgta cttttgaaat
      841 agaaaagaag aatggtaaaa aaagagtcat taatgctcct tgtggcggtc tatcgatact
      901 ccaaacgaga ctaaagcccg ttcttgaata tttctacagg ccaaagaaat ctgctcatgg
      961 ttttataaaa ggaaagagca tcattactaa tgctgggatg catattaaaa aaaattttgt
     1021 cgtaaacatt gatctagaaa actatttcga atcaataagt tttgctaggg tttatggaat
     1081 atttaaaagt aaacctttta attttgctca tcctgcagct actgttttag ctcagttatg
     1141 tactcacaat ggaaaattac ctcaaggtgc gtgtacatcg ccaatattag caaatattgc
     1201 atcagcttct ctagacaaac agctcaccca atttgcagga agaaaaaaaa tatcttattc
     1261 taggtatgct gacgacataa ctttttcttt caatcagaga aatattgata taatcaaaaa
     1321 aaacgacgac ggaagttata gtcttagtga aactatagac aatattattt caaaaaatgg
     1381 ctttaaaata aattatgata aatttagagt tcaaaccaga aatacaagac aaagtgttac
     1441 tggcttagtg gttaatgata aagttaacat taacagaaga tatataagaa ttacacgttc
     1501 aatgattcat agatggacag atgataagct aaagtatgca cttctctttg ctacagaaaa
     1561 aggatatcag gcaaaggata ataaccacgc aattcaaatt ttccgaaatc atatttatgg
     1621 aaggcttagc tttataaaaa tggttagagg gaaagactat ccaggatatt taaaactgat
     1681 gtcatacatg agtcataacg atccattaaa aacccaagaa ggattgcgag caatgaaaga
     1741 aacagaaaac tttgatgttt ttatatgcca tgcaagcgaa gacaaaaaag acattgcaat
     1801 tccaatatat gacgagttaa ctaaacttaa aatttcagcc ttcatagatc atgttgagat
     1861 aaaatggggc gactccttaa ttgataaaat aaatgcagca ctagttaaat caaaatatgt
     1921 catcgctatt ttatctgcta attcagtcaa taaggaatgg cctcaaaaag aattaagagc
     1981 agttttagcc agcgaaatat cgagtggcga cgtaaaactt ttgaccttat taaaaaaaga
     2041 agacgaggag gtcgtaaacc tatcattacc tttacttagt gataagtttt atatggtcta
     2101 tgataataat cctgaagtag tcgccaacaa tattaaatca ctcttacaac gataattctc
     2161 tcacaaaaga aaatgtgcag attgatgcgt attaagtatt aatctgcaca tacaaaaaaa
     2221 ataataaaat aatacatttt tcataacttg taggtcgcaa taactagcat aaccccttgg
     2281 ggcctctaaa cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac
     2341 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     2401 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     2461 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
     2521 cttaaaaaaa ttacgccccg ccctgccact catcgcaata ctgttgtaat tcatttaaca
     2581 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     2641 gcaccttgtc gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt
     2701 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2761 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2821 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2881 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2941 tcaccagctc accgtctttc atcgccatac ggaactctgg atgagcattc atcaggcggg
     3001 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3061 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     3121 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3181 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3241 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3301 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     3361 attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
     3421 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     3481 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
     3541 gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
     3601 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
     3661 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     3721 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     3781 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     3841 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     3901 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     3961 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     4021 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     4081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     4141 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     4201 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     4261 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     4321 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     4381 atcagataaa atatttct
//
