LOCUS       sequence-318902-        9684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 1 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG017 (AVAST type 1)
FEATURES             Location/Qualifiers
     source          1..9684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7142..7189
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7409..8068)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7983..8002
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8069..8171)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8563..8580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8697..9242)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8777..8796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9467..9571
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acatattttg cctgacttac gtgagctgag ttttaacggt taccggttac gtctggtcat
       61 ggcaacacat attgattcag atcacattgg tggtctcgtg gacttctttc ttgtaaatgg
      121 acacgcagca gagcctgcag tgattactgt tgaccgcgta tggcacaaca gcctcagggc
      181 gatgacgaga cccgaaaata atgcacaaaa agtggattcc cgagaaatca ctgacttttt
      241 gagacggaga tatcatgtcg aagccgataa agccaaaccg catgaaatca gcgcgcgtca
      301 ggggagttca ctggctgcca gccttctggc tggcgattat cattggaatg agggaaaagg
      361 gtatcagtgt atctgcaccg gtacctccat tcccaacttg atgtgcgata acagtctaac
      421 aattctgagc ccctctaagg agagaatttc agcgctctgc ctgtggtggc gcagacaact
      481 tgcatcgctg ggcttttcgg gacggtcctc ctcgagtgag gcatttgatg atgctttcga
      541 atttttttgt aaaagggaag catctcaggt tcctcttccg catgtcatca atgcaagaac
      601 accgttgctt gagagggatt atgcacggga tacctcgcca acaaatggca gttcgatagc
      661 gttcagtctg gtgctcaata agaagagaat attgatgcta ggagatgcct gggcggaaga
      721 agttgtgaca tctctgggtg ccagtggggc gtcccatcat tttgatatca ttaaaatctc
      781 acatcacggt agtattagaa acacaagccc gaatctttta aagatcatag atgctcctgt
      841 gtacctgatc tcaaccgacg gaaaaaagca tgccagacac cctaacctgg cggttctgaa
      901 agcgattgtg gacagacctg cggcgtttac gcgaacgctc tattttaact atgccaacag
      961 cgcatctgct tttatgaaaa attacctttc tgcaagtggt gcacaattca gaatcattga
     1021 aggatcaacg gattggataa cactgtgaga tatgctgcta ctgaaactga aataaggaac
     1081 gcaactgtac tcattgaatg cgcgggttac actggttccg gaaccctgat cgcagcagac
     1141 aaggtcctta cggctgcaca ttgtgtagta tcggatgatc ctgagacacc aattacagtg
     1201 acattttttg gtgcggatga agacgtctgt gtcaatgcga caatttcaga aatagataca
     1261 tcgtgcgatg cctgtctgct aacactttct gactctgtcg acattccgcc tattacactt
     1321 atgacacagc cggagcgaga gggaagccaa tggaaagcct ttggctatcc ggcatcacgc
     1381 aatgggccat cacattatct tcatggcact ataagtcaga ttttaccaag gcttttccat
     1441 ggcgttgata tggatttgtc ggtcagtgcc gattgtgttc tggaagagta cagtggagtt
     1501 tctggtgccg ccattctatc agaaaataaa tgcattgcga tggtgcgcat caggatggat
     1561 ggtggactag gtgcagtaag tcttgataag ttaagcggtt tgctgattcg aaacggcctc
     1621 atcccagatg acattgcatc cctgccagat tcatcactgt cgggtgaagt tgtcctgaac
     1681 cgcacagaat ttcgcgacaa ctttgaatcg ttcgtcctgg agcacaaggg acgtgcagtg
     1741 cttttggaag gtagtcccgg ctctggtaag actaccttct gccgccatta tcagccccgt
     1801 agtgagcaac tcgcagtggc gggtgtctat gaatttacac cggaagacgg tgctggtacg
     1861 acattcaaaa ttcttcctga ggtatttgcc gattggctgc ataaccaggt ttctatactg
     1921 ctttcaggta ggcctgctcg cagggaggaa acagaaaaga tcaatctgac ccaaaaggtg
     1981 tctgaccttc tacatacttt ctcagattac tggaagcaca aaggaaaata tggcgtcatt
     2041 ttcattgatg ctgtgaatga ggcaagcgag tgcggggatg aggcagtatc gcgctttaca
     2101 gcattactgc cggtgacact tccggagaac gtcaaacttg ttttcaccgc accatcatta
     2161 tcatcagctg gtaaggcttt ccggcactgg ctcacacctc aggattgtat cagcctaacg
     2221 cttttaagcc atagggaggt gttacagcta acagctcgag agcttaaaac ttccgcccct
     2281 tctttgtcac tactcacacg agttagtgat atagctcagg gccatccact ttatctccga
     2341 tacattcttg ggtatctgaa agcgaatccg gatcaggtta atctggagat attcccggtt
     2401 ttcagtggca gcattgaaac ctactacgaa aggctctggc aggggctggt taaggatgag
     2461 agcgctgtaa atctgctcgg tattctctcg cggatgcgct ggggcattga tatttcatca
     2521 ctgatccctg ttctaacacc gcaggaacag acggtgtttg ttccaaccct tgaccgtatt
     2581 cagcatctgc ttcttaatga taaatcatca gcattgtgcc accaatcatt tgcggcgttt
     2641 atcaacagta aaacggcggt aattaactcg ctgctgcacg gacgccttgc cgacttctgc
     2701 cttaccagtg gagagagtta tggcctgatt aatcgcgctt atcacctgct cctagcctct
     2761 cacgacagac atcctgaagc cgcattggtg tgcacgcagg aatgggctga cgcctgtatc
     2821 gtcaaggggg ctcagccgga tattctaatt cacgatatcc gtcagaccct gaagaacacg
     2881 cttattcgtg ccgatgcagt ggcatcgatt cgtctgttgc tgcttttcca acgcatgacc
     2941 ttcagacacc attttttgtt tctgcagtca gcttatcact caggccttgc cctggctgca
     3001 cttggcagac cggatgaggc ccttgagcag ctcataccat ctggaagcct cgttgttgat
     3061 gcagttgatg caattgtcag cgcacagact ctcgcgcgta tgggaaacag tgaacacgcg
     3121 ctgaagctat tggaaaaggt gaagtcagct gtcgaccaag aatttgaacg caatcccgtc
     3181 aatctatctg attttatcgg cctttccctg gcttgggtga gagctgagct gatggctggg
     3241 gtggttgatg gccacggacg cacacgcgag gttgttgagt atttgtacgg ttgtgggcaa
     3301 gtcgttcgcg ataattttga acaatcagcg catagtaaat cagcatatac acgcgctttt
     3361 tatcctcttc aggcagaaat ggaagccgtg aacatagcct ttaatgaccg ctccgtatct
     3421 ttacggacgg ttaaagaaaa gtttggtagc ttaccggaaa atattcttga tctgatgctc
     3481 agttcagtta tgcgggcaca tgacatcatt ctgcaacatc agttgccgat gccccagcat
     3541 gctttgcaac ccgtttggta caatctggac agattacttc atactgatat tccgtattcg
     3601 aacgaaattc gttttaattc attaagtagc cttatttttt tcaatgcgcc ttctgctctt
     3661 attatcagga tggcgggggt attttctttc gaagtagtac ccgaaataac gttgctcaat
     3721 gaagaaaatg agatagcagc agacagcatt gacgttagtg aacagggaca actctggctg
     3781 gtgagcgcct accttaatga aacgcaaccc tgtcccgata ttaaacatcc gagtcaggga
     3841 tgttctgaat ggctcaagac attgactgag gctatttttt ggtacagcgg gcaggcgcgc
     3901 cgggcagtta ttgacggcaa cgatgagaaa aaagaactgc ttttagtcaa ggtgcagaat
     3961 gatattctcc ctgctctttc gtactcgctg gaagagcgca tggcatggcc gaattcatgg
     4021 gcaatgcctg aacagattat ccccatgatt tacgaagagt tagtaaacat gttcggcgca
     4081 tgctggcccg ataagatatc agtgatcact gatttcattc tggctcatac gcctcagcaa
     4141 tgtggacttt attccgaggg gtacaggcgt ttactgaaca gagttattca gactcttcta
     4201 aatgagcatc ggtttttggg gcaatctgat acgacatttc aactacttga gacgttgcat
     4261 gcgtttgttt ctgcttttac tgagaatcgg caggagctgg ttcctgaatt actgaatatt
     4321 attccagctt atattagcct tgatgctcct cagctggcac aggacactta cactgagctt
     4381 ttaggtgtgt cgatgggccc tgactggtac aaagaagacc aatttgccct catgacaact
     4441 atgctgcgcg tgataccaca gcatacagac acaaatacta cactttcaca agttgcagga
     4501 ttccttgaac atgcttcggg tgaaatgaca tttaggcgtt atgttaggca ggaaaaatca
     4561 cagtttattg gcgaacttat tcgtcgtggg aattatgcac acgggtttaa ctattatcgt
     4621 cagcagtcct gcggatccca tgaggaaatg ctcacccaac ttagccaccc agctgcagat
     4681 agccctcatc cattgaaagg catgcggttc ccggggggag cgctggatga ggaacatgct
     4741 gtagaatgca ttgtcagtga actgcgaaac agagtcgact ggcggcttcg ctggggactt
     4801 cttgaaatat tcagctttgg cagtattggt aatcttgcag tgccctttgc tgaacttatc
     4861 aatgaatttt ctgcagacac tgaagacctt aatgaaatac ccaaaaggtt gcacaacatt
     4921 ttacatggtg atgtgccttt ctcagaacac agaaatttta tcaaaaattt cacagagcac
     4981 cttgcagaca accataagcc actctttgct gaatttatca gtttgctatc cgaagacact
     5041 agcgataacg acgttaagcc tcccccctct ggtgatgcta accagaaggg tactgatacc
     5101 tcagatgatg tggcaatgca gccaggactt tttgggaagc gttctgcgat caatagggct
     5161 gaagcctgca tggaaaatgc ccgaaaagcc gcagcacgca gaaacacagt tcgtgcaagt
     5221 gagttagccg ttgaaagcct gcatataatt caggatggtg actggtcagt ctggagaaag
     5281 aacaaccatc tggcggaact tacacggacg tacatattgg acaactctgc ggatgcaggt
     5341 tcggtcattc gtgcttatgc ttcgcttgta gaaaaagaac gttatgcccc ggcatgggta
     5401 attgctagtc atctcatcga aatagcagcc agtaaattct ctgatcaaga agcccaagct
     5461 attaaccaga tcgtacttga acacaaccgc cacatgcttg ggaataccga agcggatgct
     5521 gcgcattttt cttttcttaa tgaacctgat acctcagatg caggtgaaga aacactctat
     5581 tttctgtttt ggctgctgga acacccactg aaattcagac gcgaacgggc tctggaagta
     5641 ctgaagtggc ttgcatcaga cgatgataag attctgggcc aatgcgtgac ggaggcactc
     5701 gtttcagaca ttgcctcacg agctgaagca ctaatggcat tgacagactg ggtgtcagct
     5761 agatctcctc agcgaatatg ggactttata gttaaagagc gcagcctttt tgaatggctt
     5821 gaaggcacta ctgcactaag ccaagtccat ctcctggagc gagtaaccag cagagcggga
     5881 tttgttttaa gaaatgagat tgccgcattt gagcgacccc gaaagctttt actgacatca
     5941 gaagcctctg gacaacggaa tattccagaa aatttaccaa catgggtgca atccttgtcg
     6001 cagacccttg ccgtgatgga aaagcaggga atagatatcc cagctttgct taccttactc
     6061 gaaaaacggg ttttacagca gagtggattg gctgatatca cggtggcttt tgagctggaa
     6121 aagttacttg cgcgtggttt tactgtgaat agaacaccaa gtcaccatcg ctgggagacg
     6181 atggtgcgat ttgcattaaa ccagatcata catgaggcgg ccgcacagga tgaactgcaa
     6241 aacattgaac ccttgctacg tgcctggaac cccgcgtcag aggagtgtgt tgagccgtgg
     6301 gaggtttgta accgggcaaa acagattatc tgcgctgtta tggaaggtag acatcagcaa
     6361 gcttcgggca tagaggatgg ctttttcttg cattatcttg atgaagtgga ggtttcccga
     6421 gaaggtcaaa cgcatctggt ggaaatctca gcggtgttaa cgacagctca taatggtcat
     6481 gagagcctta gaccaggtgc agaaagcgaa tttaatgcaa cacagacacc tgatatagag
     6541 cggacgctta gtgtgcacct tacatgccag cgagtcaaaa tgcagccttt gctttttggg
     6601 ggagctacgc ctgccgcagt gtcgaaaaag tttatgcaga tgactggaac gttgccttca
     6661 gactttattc gcaggcaatg gcgaagcggg cgttctctta gtaaaaacag atggggggaa
     6721 ccaataagca gaggaagtct gttactcatg aaaagaacaa ctaccctccc tccaggactg
     6781 ggcttagcgt ggtatgtcac tgtcgatggg aagttgatga atatattttc atatgccccg
     6841 aggaggagat aatgaaatac agttcaatgg aaacgccaaa aacgcgagag gaatttgagg
     6901 ctcgctgttt tcacctgctc aatgcgatca agttaggacg gtatcatggc attccgggtg
     6961 aaggtaacaa agagcaggtt ccttttctcc ctaacggacg agttgatctg gcaaacattg
     7021 ataccatgac tcgcctctcg atgaactcgt tatatgattt ccactataac agggataatt
     7081 atccgcagtt tgatctctct gaaaatgacg agaatgaaga ggctacggat tgacgcaata
     7141 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
     7201 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7261 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7321 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca ccaggcgttt
     7381 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcaatact
     7441 gttgtaattc atttaacatt ctgccgacat ggaagccatc acagacggca tgatgaacct
     7501 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atagtgaaaa
     7561 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     7621 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     7681 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     7741 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     7801 ggtgaacact atcccatatc accagctcac cgtctttcat cgccatacgg aactctggat
     7861 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     7921 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     7981 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8041 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8101 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8161 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8221 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8281 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
     8341 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
     8401 cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg
     8461 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     8521 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     8581 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     8641 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     8701 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     8761 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     8821 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     8881 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     8941 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9001 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     9061 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     9121 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     9181 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     9241 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     9301 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
     9361 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     9421 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgccgcg gaacccctat
     9481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     9541 aatgcttcaa taatattgaa aaaggaagag tatggtagcg ataaaaatgt atccggcaaa
     9601 ggatggggat gcttttctta ttatttgcga tgaggaaaaa agtgcatttc tgattgacgg
     9661 aggctacgcg gaaacgttca ggca
//
