LOCUS       sequence-318903-        6434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the upx defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG031 (upx)
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4200..4247
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4467..5126)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5041..5060
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5127..5229)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5621..5638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5755..6300)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5835..5854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
       61 gtgagctaac ttacattaat tgcgttgcgc agggctgttt ggttgaatta aaaatacgaa
      121 ctaaaaccaa caagagtcgg aaaaaacttc aaaatgctgc ttatggataa tagtcatctt
      181 aaaaatgtac ggaaaaagag actaaaatca gaaaaacatc tgttatacat tgacttaaag
      241 tcatcatctc cgctatgagt cctcaatcca agttgacaaa tgtttagcca ggagttcccg
      301 tgaacgagca tctctctcat atggatgtac ataccttgtt tgaagaaatg gacgagcagg
      361 ctgatggaat aacgtttaaa tactcatttg atgacatagc aaagagcaac gcattggttg
      421 tcactgagtt tgtcaatttt gagcgtgaca gcacggtagc tttactcgcc agccttctta
      481 ctctcccggc acaccaatct cagtgtttgc gctttgagct tctgacgagc cttgcactaa
      541 ttcactgcaa aggtcagcag atagcaaata tcgatgacgt gaaacgctgg tatgtcacta
      601 ttggggagtc gagtagtatc gttggagaag atcctgctga ggacgtcttc gtcgcccttg
      661 ttgataataa aaaaggtgat taccgtgtgc tagagggggt ttgggaggcg gcaggttttt
      721 atacacaatt aatggtcgaa attgtatccg acatgccgga tacgcaccgc tatcgctcgc
      781 tgaaacttgc tatacaggca attctccgtc tctcagatgt catttgtgct cgctctggcc
      841 tttatcgttt tcaggaaggc gcagacgaat tccctgactc tcttgacacc gctggtcttg
      901 atgagaaaac gctctgttca agggtaacgt tgtccgagcg ttctcttcga gctgagggga
      961 tcaaacttgc tgacttagca cctttcattc ttgaaccttc tcatataagt atgcttggaa
     1021 atcaggtccc tggggaggga atgcttgaac aacggccatt gctccgcaca cgcgatggta
     1081 ttgtggttgt acttcctacc gccatgacca ttgcacttcg ccaggcagtg ataacatttg
     1141 caaagcgcac agaagaattg agcgagctag acaaagcgtt agctaacgtc tacagcctta
     1201 ctttctccga gatgccggtc ttcggtaatg gaggaaggtt aagaagactg acatgggaga
     1261 agtacaaaat gagccgaaca acgatggtaa cctccatcgt ggatgctggt catttgatgg
     1321 tacttcagtt cgttttgcct tccatacagc aatatgccga taccggtttc aacaacttgc
     1381 tacagctaga tgaagagacc acgcaatttc tagataactc tgttgaacaa attacagttg
     1441 acctcgccaa acaacccggc tttcagcgtg gcatcgtcgt gcgcattgca tgtgggtggg
     1501 gggcgggttt tatgggggtc cctccccaac tgccagatgg ttggggattt gaatggatgt
     1561 ctggtgcgga ctttgtccgg ttcggggcat tacccgatat gtcaccaatt gccttctggc
     1621 gtgtgcaaga cgcagtcgaa acgatcaggc aagctggtgt tcgattaatc aatatgagcg
     1681 gaactctcaa tcttcttggg tggatacgtg ccaatgatgg ccatatggtt cctcatgacc
     1741 agttaccaga tgaccgtatc acaccggaac acccgctaat gttaatgatt cccacgaatt
     1801 tactccgtgg tatacgaata gcggcagaca caggatatga ccggcatcgc attagtgaca
     1861 acaatggtaa atggcatcga gtgatgaggc cttcggcaga agatttcttt cccaccgagc
     1921 gtcagagcaa gtgctacgca tcaattgatg atcttgaagc gcaacggctg acctgtgtat
     1981 atgaggggca gggtaatctt tgggtaacgc tcgaagctcc agaaatggaa gattggatgc
     2041 tcctcgttga gcttgccaaa atggttcgaa catggattgg gcggattggc gaggcactgg
     2101 aggtcttgag tgagcaacca ataaaaaaat cattaaaggt gtatctgcat tttgatggta
     2161 acgacaatat cggcagattt gatggtgaga atttttctga tgatatgaat acattttggc
     2221 gacttgaacg aatccatgag catggggcga ttcgtgtggt tcttcaagat gggtatcttg
     2281 caggttttcg tctaccggat aaccgtgcag aacgagctct ggtgcgcgca ctcggtacgg
     2341 cgtttgccac acttcttcgg atgaaagagc cagtagacaa aggggtcact gttgagcaga
     2401 tagcggtgcc caatgacaga gcgcgcagct tccacataat gcaggcttat gacttcaacc
     2461 aatatttagg ccgttcacta actaaacgtc ttttagctat tgaagatatc gactcagccg
     2521 cagcccgaat tgagctagca tggcgtgctg tttcgacaga tgcaccatca cgatatcagg
     2581 gtaaaaagga agttggaaag ctccttaatg atgtggttga tgtgctgatc caagacttac
     2641 taagcgaact ttcaagattt gaccgtaaac agacagtaat gcgattactt gaaaacgttg
     2701 taaaggcacg ttgtgaagag gcgcactggc gtagtactgc agcagcggtc cttggcttgc
     2761 atgcaggaga agagggtgtc gaagagacga tagctcaaga aatgagccgt tatgcgggcg
     2821 cagcgttaac ttcccggcta atcattgaac ttgccatctg tgtgtgcccg acaagcggtg
     2881 gaattgaacc ttctgatatg gcactcagta aacttcttgc acgggcatca ctgctttttc
     2941 gcataggtgg tatgtcagat gccgtacgtt tcggtgcttt gcctgctgat attcgcatct
     3001 cccccttagg tgatctcctc tttcgcgatg aactcggcaa aatggtgctt gaaccaatgc
     3061 tttcaaaagt tactaacgaa cggtttgagg aacaagcggc acaattcgag caacactatg
     3121 tgaaaactgc cggaggggat gatgagaata gcaaacaaga tagtgttgcg gctgaaacca
     3181 ccgaggacca aaccgatatt ttccttgcat tctggaaagc agaaatgggc ttcactctcg
     3241 aggatggaat gcgatttatc cagttccttg agtccatcgg aatagagcaa gaatcagcaa
     3301 tcttcgagat gcgaagaagc caattagcgg atgctgctaa atcggctggg ctcgcagatg
     3361 aaactattga tgcgttcctc aaccagttta tccttagcgc gcgtccgaaa tgggatgtag
     3421 tgcccgatgg atttgacctt tctgatatat atccctggag gtttggccga cgcctttcag
     3481 ttgctgtacg tcccttgtta cagattgaag agagtcacga tccactaatt gttatcgcac
     3541 caggactctt gaatctgtcc cttaaatacg ttttcgatgg cgcatacact gggcaattta
     3601 agcgtgactt ctttcgcaca gagggtatga gagacacttg gttaggtgga gcgcgggaag
     3661 gacacacatt cgaaaaaact ttggagagag aacttcgtga aataggctgg acagttcgac
     3721 gtggcatagg ctttcctgaa attcttcgca ggaatctacc aggtgatccg ggggatattg
     3781 atcttcttgc ctggcgctca gaccgcaatc aagttctcgt tatcgaatgt aaggacctct
     3841 cacttgctcg taattactca gaagttgcct cgcaactatc tgaatatcaa ggtgatgaca
     3901 taaagggcaa accagataaa ctcaagaaac accttaaacg cgtattacta gccaaagaaa
     3961 acatcgataa ttttgccaag ttcacttcga tagcgaatcc cgagattgta tcgtggctcg
     4021 ttttcagtgg agcatctccc attgcctatg ctcaatccaa gattgaggct ttggcaggaa
     4081 ctaatgttgg ccgcccaagt gatcttctga acttttgata gatatgctgt gcgataagac
     4141 gccctggcaa ctaagttaat cgttcctact actgatagtt ttaaatcaag gcgcaataac
     4201 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg
     4261 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     4321 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     4381 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
     4441 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcaatactgt
     4501 tgtaattcat ttaacattct gccgacatgg aagccatcac agacggcatg atgaacctga
     4561 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg
     4621 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     4681 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     4741 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     4801 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     4861 tgaacactat cccatatcac cagctcaccg tctttcatcg ccatacggaa ctctggatga
     4921 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     4981 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     5041 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     5101 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     5161 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     5221 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     5281 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     5341 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     5401 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     5461 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     5521 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     5581 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     5641 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     5701 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     5761 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     5821 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     5881 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     5941 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     6001 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6061 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     6121 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     6181 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     6241 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     6301 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     6361 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
     6421 cccgcgccca ccgg
//
