LOCUS       sequence-318904-        5939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the TerY-P defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG030 (TerY-P)
FEATURES             Location/Qualifiers
     source          1..5939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3925..3972
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4192..4851)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4766..4785
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4852..4954)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5346..5363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5480..5939,1..86))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5560..5579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
       61 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
      121 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
      181 agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
      241 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
      301 tgcgttgcgc acgtgatgaa tgaagcggct aaatacatta atgataatta taatttaatt
      361 cattaaaatc agtaatatat aaatataaaa gttgtgaaat gtgatattcg tcaaagcatg
      421 tcaaaaagtt ttgactgttc tttaggcatc attcgcaatt gtctaacaac ttgataggat
      481 aggaacaatc tcaaaaagga aaatgacata tggcatacga agctcaaatc agccgtacta
      541 atccagcagc atttcttttc gtcgtcgatc agtcaggttc aatgtccgac aaaatgtctt
      601 ccggccgaag caaggctgag tttgtcgccg atgctcttaa tcgaacttta atgaacctaa
      661 tcactcgctg cactaagtct gaaggcgtac gtgattattt cgaaattggt gttttgggtt
      721 atggcggtca aggggtttct aatggtttct ctggttcact gggaggacaa gtcctcaatc
      781 caatttctgc tctcgaacag aatccagcca gagtagaaga tcgcaaacgg aagatggatg
      841 atggagctgg cggaatcatc gagacagcaa ttaagtttcc agtatggttc gatcctattg
      901 ctagtggcgg cacgcctatg cgtgaagccc tgaccagagc cgccgaagag ttggtgactt
      961 ggtgtgatgc ccatccggat tgctatcctc cgactatcct gcatgtgact gacggcgaat
     1021 caaacgacgg tgacccggaa gagattgcca atcatctacg acaaattcgc accaatgacg
     1081 gtgaagttct gattcttaat atccatgtca gttctctcgg aaatgatcca atcagattcc
     1141 cctcctcaga cactggctta ccggatgcct acgctaaact gcttttccgt atgtccagcc
     1201 ctcttccgga acatctggtg cgtttcgcgc aggaaaaagg tcatacggtc ggtatagaat
     1261 ctcgtggatt catgttcaac gctgaggctg ccgaactcgt cgatttcttc gacatcggaa
     1321 cccgcgcttc tcagttgcgt tgattcagca atgaaactgg agttcttagg gacagttccg
     1381 aaagatcctg aataccctaa ggcgaatgaa gataaatttg ccttctccga agatgggaga
     1441 aggctggcgc tatgtgatgg cgcgagtgag tccttcaact caaagttatg ggccgatctt
     1501 cttgctcgta aatttactgc agatccgaaa gtaaatcctg aatgggtagc atctgcttta
     1561 gcggaatatt ctgccacgca tgacttccct tctatgtcct ggtcccagca agcggcattc
     1621 gaaagaggca gttttgcgac actaataggt gtagaggaat ttgaagagca tcaggcggta
     1681 gagattcttg ctattggaga tagcatcacc atgctggttg attgcgggaa actcatttgc
     1741 gcatggcctt tcgataatcc agaaaaattt aatgagcggc caacactgct tgctacgctg
     1801 tacgctcata acaatttcgt cggtggaagc actttctgga cacggcatgg gaaaactttt
     1861 taccttgaaa aactcaccca acccaaactc ctctgtatga cagatgcgct cggcgaatgg
     1921 gcactgaaac aagcgctggc agaggattct ggttttatcg aattactttc gctgcaaact
     1981 gaagaagagc ttgcagagtt agttctgaga gagcgtgcag caaaacgtat gcatatcgac
     2041 gactcaacgc tgcttgtact atcgttttaa cgcggaaagt aaagatgcct tacccatctc
     2101 ttgaacaata caaccaagcg tttcagctac atagtaagct gctaatcgat cctgaattga
     2161 aatctggtac cgttgccacg acagggttgg gtctccccct agccatcagc ggtggctttg
     2221 cactgaccta tacaatcaaa tcaggcgcta agaaatacgc cgttcgttgc tttcatagag
     2281 agtcaaaagc cttagaacgc cgttatgagg ctatatccag gaagatttca agccttcgct
     2341 ctccctactt tctcgatttc cagtttcagc cccaaggggt caaagtcgaa ggaatatcat
     2401 accctatcgt caaaatggca tgggccaagg gagagacgct aggagaattc cttgaggtca
     2461 acaggcgttc tgcacaagca atagcgaaac tatctgcatc gattgaatca cttgccgcct
     2521 accttgaaaa agaaaaaatt gcacatggtg atttccagac tggaaacctg atggtctccg
     2581 acggaggtgc aaccgtccag ttaatcgact atgacggcat gttcgttgat gagattaaga
     2641 cattaggaag ctcggagttg gggcatgtca attttcagca tccccgtcgt aaagcaacga
     2701 atccgttcaa tcacactctg gatcgtttct cactaatttc actctggctg gctcttaaag
     2761 ccttgcaaat cgatccgtcc atttgggata aatcaaattc ggaactggat gcaatcattt
     2821 ttcgagctaa tgactttgta gaccccggtt catcttccat cttagggatg ctatcgggaa
     2881 ttcaacagct ttccacccat gtaaagaatt ttgccgcagt ctgcgcttca gcgatggaaa
     2941 aaacgccttc cctcggtgac ttcattgcaa gtaaaaacat tcccatatcg ctagcttcga
     3001 tcagtatgaa tggggatatt ccagtcagca ggctgaaacc cggttatatc ggtgcctaca
     3061 ccgtcctgtc agccttggat tacagtgctt gccttcagcg agttggtgat aaagttgaag
     3121 ttatcggaaa gattattgac gtcaaactca ataagacccg aaatggcaaa ccatatatct
     3181 ttgttaattt cggagattgg cgcggtaata tctttaaaat atcaatatgg agtgaaggca
     3241 ttagcgcttt accttcaaaa cccgatgcct catggatagg gaaatggatt agtgtaatcg
     3301 gccttatgga accgccttac gttagcggga aatacaaata ttcacatatc tcaattacag
     3361 taacgactat cggtcaaatg accgttcttt cagaaccaga tgcccgctgg cgtcttgctg
     3421 ggccaaacga aagtcgacaa acattaactt ctactagcag taatcaggaa gccttggagc
     3481 gcattaagag taagagcacc acttcaactc ctatgcccat gaacactaac gccacaactg
     3541 caaatcaggc aatccttaac aagttacggg cttctacgca aactgtagcg gcagcaagag
     3601 cgcaaactca gcatgtagta cctaataaat catcaacgca ttatgtggca ccgacgggaa
     3661 catcagcttc gcagccagtt caaaatattc cgagccctgc tagtacctca aagcagcaaa
     3721 cctctcaaaa aaatatagtt acaaagattt tgaaatggct ttttggatga ttggtacttg
     3781 taaagaacaa gcgcaatttc agtggccgta tcacttgcgc ttgaggtgcc tgcgggtatg
     3841 atcttgcgac atacaccact aaaacgaatt cgtggcggca cttttagcct gcccctgtgt
     3901 tttcccgagg atttaccgca ataactagca taaccccttg gggcctctaa acgggtcttg
     3961 aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta
     4021 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     4081 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
     4141 ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     4201 gccctgccac tcatcgcaat actgttgtaa ttcatttaac attctgccga catggaagcc
     4261 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     4321 ataatatttg cccatagtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     4381 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     4441 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     4501 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     4561 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     4621 catcgccata cggaactctg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     4681 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     4741 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     4801 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     4861 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     4921 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     4981 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     5041 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta
     5101 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     5161 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc
     5221 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     5281 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     5341 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     5401 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     5461 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     5521 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5581 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     5641 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     5701 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     5761 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     5821 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     5881 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtag
//
