LOCUS       sequence-318906-        5400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ppl defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG032 (ppl)
FEATURES             Location/Qualifiers
     source          1..5400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3092..3637
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3538..3557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3754..3771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4163..4265
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4266..4925
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4332..4351)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(5145..5192)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 accgcagagt cctggataca ggcctgtgcg atcgtgctgc atactgataa catattctgc
       61 atcgccgtta accactatag gggcagagtg cgacacgata atcagctgcc ggcgtttctt
      121 gttcgacatg atggcaggga tcacgctcat gctcagcatc aggcagtcca ggtcatcctc
      181 cggttgatcc agtagtagag gatcggtgcc ataggataag aggaactgca gcatactcgc
      241 tcccttttgc cccggagaag caaaggcaat gtcttccatc tgccccccgg gggtctggta
      301 acgtatgtgg atgccgtcat caggatacca gcattgcagg gcatccagct gctggtcatt
      361 cagactcctg agacgattcc ggaaagaacc atgaagcccc gccccagcct ctgaattatc
      421 cagccggatg ctgtgatgca gcgctttcag ctcgtccagc gccctgtatt ttttctctgt
      481 tgcggcaggc aacgggctga acggacgttc actgataaag tcgctcagta atccgctccc
      541 gtcactgtta tcgtagatac gttcggcaaa attactgatg ccggtaaccg tctggtaacc
      601 agatataaca tcttcataag gggcgcagag gggaagaatt ttgatttcca gagcgctgag
      661 cgacagggag gagagaaacg ccttccggtt atctgtcaat gccattctgt gggcacgcat
      721 ttcagccagc aactgctcag accgtgcaac agaggcagag atcgccccat caagaccgtc
      781 gtaatttctc agctcatttt tcagtgactc acagcgtgcc atcagttcgt tgagaacatc
      841 gggattaagc cctctggccc gcagggaaac agcttcactt tctacatttg tttcctggtc
      901 actcactgct gttcggaggg caataaaaga ttgctctctc ctgatacccg cgagctctgc
      961 agcatattct gccaggagag tattgagccg ctggtcatac tgctgttgca cagatgacag
     1021 gcgcgccata aatgccatca gcagaccgga aggttccggc gggatatcgg atctctgcag
     1081 cggttcttct gccagagcct gaatggctgc aatacgccgc tcaaagtgct ccaggggtaa
     1141 ggttgctgcg gacagctcgt ttctggcgag ggccagctgt ctgcaaaccg gataataggc
     1201 gcttgactgc agctgactga cggcacgttc agcatccgat aattcccccc gcagcgaatc
     1261 cgcacttccc tgtctggcac gcaggccccg caacgtgatt tgttcattca gatattccct
     1321 ttccagctga tcccagcgct ctttccaggc ccgtttgtta accaccgggc tctcatcaca
     1381 gacgcgcagg aatgcaccag tatccgaagc cagctcatag agcattttct ggctgtaaat
     1441 cgagagtgga aaacggtcag ccgaccagtg actgtcaggc atccattctc cgtcactgaa
     1501 gatatgtaat tcatgcttac tgtctggtcg ccaactgagc cggaaatctg tgccttcttt
     1561 gtggaaaata cattcgatga aggaatccgc ttccatcccc gtccgaatga actggtccag
     1621 cttactcccc tgggtcgcag tgagaccttc tgttttgcgc attgcaagac gaatgctttc
     1681 aatgagcgtg gacttcccgc tgcctcttga gccgattaca gcgttataga acgggctgaa
     1741 ttccaccgag gccgaatcct gacccacagg atggcagtgg cgggttctga atttcaggct
     1801 gcgcaaatat gacgcaggtt cctccggagg gagctgatca aacagcacac agttttccgg
     1861 atcgagcagt gcatgcctga gtccgtcaaa atcaggggat gacattttca gccagcaggt
     1921 tcgcacgccg gcttgttcag gatggtgcgc atcagagccc cgtagccacg gtaaattatc
     1981 aatcagccgc cggcgggttc cgtcggtgat atcctccacc cggtgtcgaa tctcaacggc
     2041 atggggactt tcctgaaagg ctgcggtgag ctctgcctga ttagtcagac ttaagacacc
     2101 tttggctgca tcaatgtgcg caagaataca aacagccttc ggattacggc ggatattact
     2161 gatgatgccg gcggggccca gttgaagaac gagctgatgg ttagggactt cactcggaat
     2221 ggggctatta ttattgcact gggccagaag cctttccaca tcggcacttg tactgtgcgt
     2281 gtgcagcaca gccagaatat gtacgttacc ggtcgctgtc agttcaaccc ccggaaagag
     2341 tgtcagtggc cgaaattccg gcaggtcgcc ggtgctggcg tcccgggaca tattctccag
     2401 ctcacccttc aacacgtcga cccaggctcc gctgttatga tcgctgatta caacacaatc
     2461 gacatgctgt ttcatataag ccagaagcca ctctctgggg ctgatgtcag gaattttgta
     2521 atcatgcgaa gccggagtat ggttatgaaa atcaaattta taccagcgcg aacctaccat
     2581 aacagttcct tttctagggt atttctgaag tccctcacta taaaagcttt caggctatct
     2641 gtcctgacct aatcccaata gatgtaacag tcttatagct agatccggtt aaatgtataa
     2701 ccggaagcaa gagattcccc ttaagactca cgatataatt ctttcggaga tgtcaagcgt
     2761 ccccggcaaa gactcaatga ttacggcgtt acattgatgt gttaatgttc tgctcaccac
     2821 ataatagatt cgctggtcca cggtgaatct acagtaccag tccatctgcg caacgcaatt
     2881 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
     2941 aacccagtca gctccttccg gtgggcgcgg ggcatgacta acatgagaat tacaacttat
     3001 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga
     3061 aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct
     3121 cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta
     3181 ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt
     3241 cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg
     3301 gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg
     3361 gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     3421 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     3481 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg
     3541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     3601 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc
     3661 acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc
     3721 atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     3781 tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat
     3841 gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta
     3901 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
     3961 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     4021 tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag
     4081 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     4141 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     4201 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     4261 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     4321 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     4381 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     4441 ttattcacat tcttgcccgc ctgatgaatg ctcatccaga gttccgtatg gcgatgaaag
     4501 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     4561 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     4621 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     4681 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     4741 acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa tattatacgc
     4801 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc tgtgatggct
     4861 tccatgtcgg cagaatgtta aatgaattac aacagtattg cgatgagtgg cagggcgggg
     4921 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
     4981 tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
     5041 gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
     5101 ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggtt tcagcaaaaa acccctcaag
     5161 acccgtttag aggccccaag gggttatgct agttattgcg caaagacgca gaggccatca
     5221 gtctgacatt tgcgtctttt atccaggcat tcagccgcca ggcccactct ccggtcatgg
     5281 ccgccttaag gacggttccg ttcttcagct aagaatacgc tcatagcgcg aacgaaacgc
     5341 tttttctccc ccctccattt gacggcatat cagtgccttc atcggagctt cctgcagtgc
//
