LOCUS       sequence-318907-       11930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 chimera.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX303-GFP-IQSEC1 v2N/v1C-WT
FEATURES             Location/Qualifiers
     source          1..11930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        447..650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     601..621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(695..697,698..700,701..1411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(695..697,698..700,701..1411)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(740..761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1001..1020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1348..1369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4604..4624
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    5015..5603
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5068..5088)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5486..5497)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5675..5908
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5986..6107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6023..6042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6077..6096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6147..6282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6302..6321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6303..6321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6331..6348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6331..6347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6340..6362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6489..6944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6576..6595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6786..6807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6970..7074
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7075..7143,7144..7935)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7293..7312)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8106..8694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8595..8614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8848..8865
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8982..9003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9018..9023,9024..9041,9042..9048)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9056..9072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9061..9083
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9080..9096
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9080..9096
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9115..9135
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9117..9135
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9163..9389
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9390..9570
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9617..9742
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10235..10468
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10653..10697
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10872..11372
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     11291..11309
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             11384..11779
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(11839..11930,1..26)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
       61 ggcgccggct cgagggggcc cggggttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      661 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
      721 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      781 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      841 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      901 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      961 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1021 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1081 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1141 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1201 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1261 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1321 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1381 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagctta
     1441 catgtggtgc ctgcactgca actcggagag gacccagtcc cttctggagc tagagcttga
     1501 cagcggcgtc gagggcgagg cccccagcag tgagactggc acatccctgg acagcccctc
     1561 agcctacccc cagggcccct tggtgcccgg ttccagcctg agcccggatc actacgagca
     1621 cacgtcagtg ggagcctatg ggctgtactc ggggccgccg gggcaacagc agcgcacgcg
     1681 gaggcccaag ctgcagcact cgacctccat cctgcgcaag caggctgagg aggaggccat
     1741 caagcgctca cgctcactct ccgagagcta tgagctctcc tcggacctgc aggacaagca
     1801 ggtggagatg ctagagcgaa agtatggggg gcgcctggta acccgccatg cggcccgcac
     1861 catccagacg gcgtttcgcc aatatcaaat gaataaaaac ttcgagcgct tgcgcagctc
     1921 catgtcagag aaccgcatgt cacgccggat tgtgctgtcc aacatgagga tgcagttctc
     1981 ctttgagggg cctgagaaag tgcacagctc ctacttcgag gggaagcagg tctcagtgac
     2041 taacgacggc tcccagctgg gagccctggt gtcccctgag tgtggtgacc tcagcgagcc
     2101 caccaccctc aagtctccgg ccccctccag tgactttgcg gacgccatca ccgagctgga
     2161 ggacgccttc tctaggcaag tgaaatcact ggccgagtcc atcgacgatg ccctcaactg
     2221 ccgcagcctg cacactgagg aggcaccggc cctggatgcg gcgcgggccc gggacaccga
     2281 accccagaca gccctgcacg gcatggacca ccgcaaactg gacgagatga cggcctcgta
     2341 cagtgatgtc accctgtaca tcgatgagga ggagctgtcg ccccctctgc ccctctcgca
     2401 ggcaggggac cggccgtcca gcaccgagtc ggacctgcgg ctacgggctg ggggcgcagc
     2461 cccagactac tgggccctgg cccacaaaga ggacaaggct gacacggaca cgagctgccg
     2521 gagcacgccg tcgctggagc ggcaggagca gcggctgcgg gtggagcatc tgccgctgct
     2581 caccatcgag ccacccagcg acagctctgt ggaccttagt gaccgctcgg agcgggggtc
     2641 actcaagagg cagagtgctt acgagcgcag ccttggcggg cagcagggca gtcccaagca
     2701 tggtccccac agcggcgccc ccaagagcct cccccgggag gagcctgagt tgcggccccg
     2761 gccccccagg cccctggaca gccacttggc catcaatggc tcagccaacc ggcagagcaa
     2821 gtctgagtcg gactactcag acggtgacaa tgacagcatc aacagcacgt ccaactccaa
     2881 cgataccatc aactgcagct ccgagtcatc gtcccgtgac agcctgcggg agcagacgct
     2941 cagcaagcag acctaccaca aggaggcccg caacagctgg gactcgcctg cctttagcaa
     3001 cgatgtcatc cgcaagaggc actaccgcat cggcctgaac ctcttcaaca agaagcctga
     3061 gaagggagtc cagtacctca tcgagcgtgg ctttgtgccc gacacgcccg tcggggtggc
     3121 ccacttcctg ctgcagcgca agggcctcag ccggcagatg atcggcgagt tcctgggcaa
     3181 ccggcagaag cagttcaacc gtgacgtgct cgactgcgtc gtggacgaga tggacttctc
     3241 taccatggag ctggatgagg ccctcaggaa attccaggcg cacatccgtg tccaagggga
     3301 ggctcagaaa gtggagcggc tcatagaggc gttcagccag cgctactgca tctgcaaccc
     3361 tggggtggtg cggcaattcc ggaacccaga caccattttc atcctggcct tcgccatcat
     3421 cctgctgaac accgacatgt acagccccaa tgtcaagccc gagcggaaaa tgaagctaga
     3481 ggacttcatc aagaacctcc gaggtgtgga cgatggtgag gacattcccc gtgagatgct
     3541 gatggggatc tatgaacgga tccgtaagcg agagctaaag accaatgagg accatgtgtc
     3601 ccaggtgcag aaggtggaga agctcattgt ggggaaaaag ccgatcggat ccctgcatcc
     3661 cgggctcggc tgtgtgctct ctctgcccca ccgtcggttg gtctgctact gccggctctt
     3721 tgaggttcca gacccaaaca agccccagaa actcggacta caccagcgag aaatcttcct
     3781 gttcaacgac ctcctggtgg tcaccaagat cttccagaag aagaagaact cggtgacgta
     3841 cagcttccga cagtccttct ccttgtacgg catgcaggtc ctgctcttcg agaaccagta
     3901 ctaccccaat ggcatccggc tcacctcgtc tgtccccgga gcagatatca aggtgctgat
     3961 caacttcaac gcccccaacc cccaggaccg gaagaagttc accgacgacc tgcgcgagtc
     4021 tatcgccgag gtgcaggaaa tggaaaagca ccggatcgag agcgagctgg aaaagcagaa
     4081 aggcgtggtg cgccccagca tgagccagtg cagcagcctg aagaaagaga gcggcaacgg
     4141 caccctgagc cgggcctgcc tggatgatag ctacgccagc ggcgagggcc tgaagagaag
     4201 cgccctgagc agctccctgc gggatctgag cgaggccggc aagcggggca gaagaagcag
     4261 cgccggcagc ctggaaagca acgtggaagg cagcatcatc agcagccccc acatgcggcg
     4321 gagagccacc agcaccagag agtgccccag cagaccccac cagaccatgc ccaactcctc
     4381 cagcctgctg ggcagcctgt tcggcagcaa gagaggcaag ccaccacccc aggcccatct
     4441 gccatctgcc cctgccctgc ctcctccaca ccctcccgtg gtgctgcctc atctgcagca
     4501 cagcgtggcc ggacaccacc tgggacctcc agaaggactg ccccaggccg ccatgcacgg
     4561 ccatcacacc cagtactgcc acatgcagaa cccccctccc taccaccacc atcaccatca
     4621 tcaccccccc cagcacatcc agcacgccca ccagtatcac cacggccctc acggcggaca
     4681 ccctgcctat ggcgcccacg cccatggaca ccctccactg cctagcgccc atgtgggaca
     4741 cacagtgcac caccacggac agcctcccgc ccctccacct cctaccagca gcaaggccaa
     4801 gcccagcggc atcagcacca ttgtgtgaga attctgcagt cgacggtacc gcgggcccgg
     4861 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     4921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4981 ttgttgttgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     5041 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5101 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5161 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5221 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5281 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5341 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5401 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5461 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5521 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5581 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     5641 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5701 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5881 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     5941 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6121 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6181 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6301 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6421 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6481 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6901 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6961 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7021 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7081 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7141 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7201 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7261 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7321 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7381 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7441 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7501 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7561 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7621 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7681 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7741 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7801 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7861 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7921 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7981 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8221 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8761 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8821 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8881 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8941 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9001 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9061 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     9121 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     9181 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     9241 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     9301 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     9361 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     9421 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9481 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9541 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     9601 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     9661 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     9721 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     9781 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     9841 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9901 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9961 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    10021 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    10081 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    10141 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10201 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    10261 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10321 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10381 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    10441 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    10501 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    10561 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    10621 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10681 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10741 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10801 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10861 cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
    10921 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
    10981 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
    11041 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
    11101 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
    11161 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
    11221 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
    11281 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
    11341 tcgttgaccg aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc
    11401 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
    11461 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
    11521 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
    11581 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
    11641 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
    11701 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
    11761 tggttatgtg tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat
    11821 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    11881 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
//
