LOCUS       sequence-318908-        5745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 5 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG021 (AVAST type 5)
FEATURES             Location/Qualifiers
     source          1..5745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3372..3419
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3639..4298)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4213..4232
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4299..4401)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4793..4810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4927..5472)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5007..5026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgatgttag caatgttcac tcttggctgg aatctgccag aaatcgaggt catatggtct
       61 gctttgagtg aggagcgcaa atggataaag ccctcatgag ttctttttca atgacctaac
      121 ttttgagagg cactgggtta gatcatgttt catgtttgca atacaatata tatttaaact
      181 taggtttata acttaaatgt tagttcctga tctaaaccag attattaatc actcctagag
      241 tgaaatgagt taagccaaga gttgataaaa ttaacagttt tttttacaat atctggatgt
      301 ttgctagcga acaggcatct aaaataacta tgctgagcta aacttacaat tcaaattgta
      361 ccgaggataa aatgcaagta caacatcata ctgaaccaaa cttgaagaat gagattgtgg
      421 ctttatttaa ggcttctcaa ttgatacctt tttttggcag tggatttact agagatatta
      481 gagcaaaaaa tggtaaagtt cctgatgcta ttaaatttac ggagttgatt aggaatatag
      541 cggcagaaaa agaagggtta acacaaacag aaatagatga aattctaaga atcagccagc
      601 ttaaaaaagc gtttggactt ctaaatatgg aggaatatat acccaaacga aaatcgaagg
      661 cattattagg taacattttt tcagagtgta aactctctga tcacgaaaag acaaaaataa
      721 taaatttaga ttggcctcat attttcacgt ttaatattga cgatgctata gaaaacgtta
      781 ataggaaata caaaattctg catccaaatc gagcagttca gagagaattt atatctgcta
      841 ataagtgtct attcaaaatt catggcgata ttactgaatt tattaaatac gaagatcaaa
      901 atctgatatt tacttggcgt gaatatgcac acagtataga agaaaataaa tccatgctat
      961 cctttttatc tgaggaagcc aaaaactcag ctttcctttt cataggttgc agtcttgatg
     1021 gagagcttga tttaatgcat ttatcaagaa gcacaccatt taagaaatca atttatttga
     1081 agaaaggata tttaaattta gaagaaaaaa tagctctttc ggagtacggc atcgaaaaag
     1141 taattacctt tgacacttac gatcagatat atcaatggtt aaataacaca cttcagaatg
     1201 ttgagcgaaa atcccccaca agaagtttcg aactcgatga ctccaagtta atgaaagaag
     1261 aggctataaa tttattcgct aatggaggcc ctgtaactaa aatagtggat aataaaagaa
     1321 tcctgcgaaa ttctataact ttttctcaac gagatgtctg tgatgatgca attaaagcac
     1381 tacgtaatca tgactatatc ctaattacag gtcgacgttt cagcggaaaa tctgtacttt
     1441 tatttcaaat tattgaggca aaaaaagaat ataatgcctc ttattactct tcgactgaca
     1501 cattcgatcc ttccattaaa aactcattga taaaattcga gaatcatata ttcgttttcg
     1561 actctaattt ctttaatgca caaagcattg atgaaatttt aaccacaagg gtgcatccta
     1621 gtaacaaagt tgttttatgc tcgagttttg gtgacgcaga gttatataga ttcaagttaa
     1681 aggataaaaa gatattacat accgaaattc agattaaaaa taacttgatt aatgaagaag
     1741 gtaactatct caatgataag ctttcttttg aggggctacc actttataaa tcttcagaaa
     1801 cgttgttgaa ttttgcttat cgatactata gcgagtataa aaatagacta agtggttcta
     1861 atttatttaa taagcaattt gatgaagatt caatgtttgt tttgatttta attgcagctt
     1921 ttaataaagc cacatatggt catatcaaca gtcacaataa atattttgat attcagaatt
     1981 ttatttcgca aaatgataga ttatttgaat tggagtcaac taacacagat ccaagtggag
     2041 ttataatctg caattcacca tcctggcttt taagagttat cagtgagtat attgataaga
     2101 atcctgcatc ttataaaaca gtatctgatt taataatatc tcttgcgtca aaaggatttc
     2161 ttgcagcatc aaggaacctt ataagctttg ataaactaaa tgaacttggg aatggaaaaa
     2221 atgtccataa atttatcagg ggtatatata aggaaattgc acatacctat cgtgaagata
     2281 tgcactactg gttacaaagg gctaagtcag aattaatatc ggcacacaca attgatgacc
     2341 tcgtcgaagg aatgagttat gcaagcaaag taagactcga tagtgccgag tttaaaaatc
     2401 aaacttatta cagtgccaca ttagtattag cgcagttgtc tgcaagggct ctatctataa
     2461 ataatgataa aatatatgcg ctgagcttct ttgaaagtag cctagaatcc atccggaatt
     2521 ataataataa ctcaaggcac ataaacaaaa tgatggataa aaatgatggt ggctttagat
     2581 atgcaataca atatcttaag gataatccat taatagaact ccttcctcgt aaggacgaag
     2641 ttaatgaatt aattaacttc tatgagagtc gtaagaaata atcatcctta aattaataaa
     2701 tggcaagtaa ctcattccct tgtcatttat taaactctta agagccttat cccgaaaagt
     2761 attaatctga gctaataaga ttgtttttca gctatgtcat tattttattg ccaatatatt
     2821 tacacttaag cattgacagg tagcggatag ttatttttgg cttgtaaata agccttttaa
     2881 taatagaact gtaagacaat cgctctgatt ttttgaaatt tatctcaatg ttaaattctt
     2941 ccgcttttgg cacaaacggg ctagagcaga cagatttaat gagataaggg tatagatgaa
     3001 ttctccatac ccttgaacga ttacttccca gttgatttgc ttggtttcag tcctggggta
     3061 ttaccgggtg tatccttatt atcacgtctg cgttgatcgg gttttcctgt tgattttgca
     3121 attggttttg gaccaggttt aagccccata atcgtactcc ttagccatgt cagaggttat
     3181 tcctcagtgt ggatataagg ggagcggtaa gaattatcaa gcttggatgg gcggtgaaaa
     3241 atgactactt gactattatg tgagcaatgt cagcttttga catttagagg ccagcccatt
     3301 actgaagtaa gccaaaaatg agtcgcgatg agccctcaac aatgagggcc acctcggaga
     3361 ttgcgcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     3421 tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     3481 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     3541 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     3601 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     3661 tcgcaatact gttgtaattc atttaacatt ctgccgacat ggaagccatc acagacggca
     3721 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     3781 atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     3841 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     3901 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     3961 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     4021 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat cgccatacgg
     4081 aactctggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     4141 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     4201 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     4261 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     4321 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     4381 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     4441 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     4501 tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg
     4561 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
     4621 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg
     4681 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     4741 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     4801 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     4861 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     4921 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     4981 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     5041 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     5101 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     5161 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     5221 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     5281 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     5341 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     5401 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     5461 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
     5521 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
     5581 tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     5641 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgcaact
     5701 agctaagcaa taagggcgat cggctctccc atagatcgag gccga
//
