LOCUS       sequence-318909-        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression vector containing Rab11a tagged with RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pQCXIH-tag RFPt- Rab11a WT
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(39..830,831..899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     662..681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(900..1004)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1072..1090
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        1190..1569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1570..1773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1774..1949
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    2012..2211
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2412..2828
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2758..2780
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2796..2812
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        2865..3168
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3169..3372
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3393
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3518..3527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3524..4255
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     misc_feature    join(4973..5547,5548..5550,5551..5559)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5140..5157)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5367..5386
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5585..6604
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5585..6604
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             7151..7576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7679..7701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7837..7857)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7842..8171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8022..8157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8193..8212
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8293..8310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8464..9052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8544..8563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
       61 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      121 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      181 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
      241 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
      301 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
      361 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      421 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      481 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      541 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      601 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
      661 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      721 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      781 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      841 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      901 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      961 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1021 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1081 atcacgaggc cctttcgtct tcaagaatta gcttggccat tgcatacgtt gtatccatat
     1141 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta
     1201 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1261 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1321 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1381 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1441 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1501 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1561 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1621 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1681 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1741 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
     1801 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
     1861 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     1921 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
     1981 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
     2041 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
     2101 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
     2161 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
     2221 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     2281 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     2341 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     2401 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     2461 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     2521 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     2581 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     2641 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     2701 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     2761 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     2821 taggcgccgg aattgaagat ctgggggatc gatcctctag agtccgttac ataacttacg
     2881 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2941 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3001 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3061 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3121 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     3181 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     3241 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     3301 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     3361 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3421 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
     3481 acggacctgc aggaattgat ccgcggccgc accggtcgcc accatggtgt ctaagggcga
     3541 agagctgatt aaggagaaca tgcacatgaa gctctacatg gagggcaccg tgaacaacca
     3601 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     3661 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     3721 catgtacggc agcagaacct tcatcaacca cacccagggc atccccgatt tctttaagca
     3781 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     3841 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     3901 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     3961 caacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg acatggccct
     4021 gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa
     4081 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat
     4141 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     4201 cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtccgg
     4261 actcagatct cgagctcaag cttcgaattc aatgggcacc cgcgacgacg agtacgacta
     4321 tctcttcaaa gttgtcctta ttggagattc tggtgttgga aagagtaatc tcttgtctcg
     4381 atttactcga aatgagttta atctcgaaag taagagcacc attggagtag agtttgcaac
     4441 aagaagcatc caggttgatg ggaaaacaat aaaggcacag atatgggaca cggcagggca
     4501 ggagcgatac cgagctataa catcagcata ttatcgtgga gctgtaggtg ccttactggt
     4561 ttatgacatt gctaagcatc tcacatatga aaatgtagaa cgatggctga aagaactgag
     4621 agatcatgct gatagtaaca ttgttatcat gcttgtgggc aataagagtg atttgcgtca
     4681 tctcagggca gttcctacag atgaagcaag agcttttgca gaaaagaatg gtttgtcatt
     4741 cattgagact tccgctctag actctacaaa tgtagaagct gcttttcaga caattctgac
     4801 agagatatac cgcattgttt cccagaaaca aatgtcagac agacgtgaaa atgacatgtc
     4861 tccaagcaac aatgtggttc ctattcatgt tccaccaacc actgaaaaca agccaaaggt
     4921 gcagtgctgt cagaacatat aaggatccgg aattccgccc cccccccccc cccccctctc
     4981 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5041 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5101 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5161 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5221 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5281 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5341 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5401 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5461 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5521 ttttcctttg aaaaacacga tgataagctt gccacaaccc gtaccaaaga tggatagatc
     5581 cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5641 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5701 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5761 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5821 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5881 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5941 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     6001 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     6061 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     6121 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     6181 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     6241 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     6301 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     6361 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     6421 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     6481 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     6541 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6601 atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc
     6661 gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc
     6721 cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggcc
     6781 cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc gggtgatctg
     6841 actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca gatctctatc
     6901 gagggctgca gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt
     6961 ccgagggaca ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc
     7021 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     7081 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     7141 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     7201 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     7261 cagatggaac agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     7321 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     7381 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     7441 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     7501 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     7561 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
     7621 gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7681 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7741 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7801 agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg
     7861 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7921 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7981 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8161 gcttttgcaa aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt
     8221 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     8281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8881 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9181 taaa
//
