LOCUS       sequence-318910-       10988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 splice variant 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-GFP-v4 IQSEC1 WT
FEATURES             Location/Qualifiers
     source          1..10988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..252
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             437..481
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        656..1156
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1075..1093
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1168..1563
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1623..1740
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1857..2160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2161..2364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2315..2335
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2409..2411,2412..2414,2415..3125)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2409..2411,2412..2414,2415..3125)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2454..2475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2715..2734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3062..3083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5787..6375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5840..5860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6258..6269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6447..6680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6758..6879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6795..6814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6849..6868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6919..7054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7074..7093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7075..7093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7103..7120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7103..7119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7261..7716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7348..7367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7558..7579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7742..7846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7847..7915,7916..8707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8065..8084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8878..9466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9367..9386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9620..9637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9754..9775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9790..9795,9796..9813,9814..9820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9828..9844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9833..9855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9852..9868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9852..9868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9887..9907
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9889..9907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9935..10161
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10162..10342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10389..10514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
       61 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      121 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      181 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      241 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      301 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      361 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
      421 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
      481 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
      541 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
      601 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg
      661 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
      721 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
      781 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
      841 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
      901 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
      961 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     1021 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     1081 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     1141 cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc
     1201 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     1261 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     1321 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     1381 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     1441 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     1501 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     1561 ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac
     1621 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1681 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1741 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt
     1801 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1861 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1921 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1981 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2041 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2101 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2161 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2221 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2281 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2341 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     2401 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2461 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2521 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2581 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2641 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2701 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2761 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2821 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2881 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2941 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3001 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3061 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3121 acaagtccgg actcagatct cgagctcaag cttacatgct agagcgaaag tatggggggc
     3181 gcctggtaac ccgccatgcg gcccgcacca tccagacggc gtttcgccaa tatcaaatga
     3241 ataaaaactt cgagcgcttg cgcagctcca tgtcagagaa ccgcatgtca cgccggattg
     3301 tgctgtccaa catgaggatg cagttctcct ttgaggggcc tgagaaagtg cacagctcct
     3361 acttcgaggg gaagcaggtc tcagtgacta acgacggctc ccagctggga gccctggtgt
     3421 cccctgagtg tggtgacctc agcgagccca ccaccctcaa gtctccggcc ccctccagtg
     3481 actttgcgga cgccatcacc gagctggagg acgccttctc taggcaagtg aaatcactgg
     3541 ccgagtccat cgacgatgcc ctcaactgcc gcagcctgca cactgaggag gcaccggccc
     3601 tggatgcggc gcgggcccgg gacaccgaac cccagacagc cctgcacggc atggaccacc
     3661 gcaaactgga cgagatgacg gcctcgtaca gtgatgtcac cctgtacatc gatgaggagg
     3721 agctgtcgcc ccctctgccc ctctcgcagg caggggaccg gccgtccagc accgagtcgg
     3781 acctgcggct acgggctggg ggcgcagccc cagactactg ggccctggcc cacaaagagg
     3841 acaaggctga cacggacacg agctgccgga gcacgccgtc gctggagcgg caggagcagc
     3901 ggctgcgggt ggagcatctg ccgctgctca ccatcgagcc acccagcgac agctctgtgg
     3961 accttagtga ccgctcggag cgggggtcac tcaagaggca gagtgcttac gagcgcagcc
     4021 ttggcgggca gcagggcagt cccaagcatg gtccccacag cggcgccccc aagagcctcc
     4081 cccgggagga gcctgagttg cggccccggc cccccaggcc cctggacagc cacttggcca
     4141 tcaatggctc agccaaccgg cagagcaagt ctgagtcgga ctactcagac ggtgacaatg
     4201 acagcatcaa cagcacgtcc aactccaacg ataccatcaa ctgcagctcc gagtcatcgt
     4261 cccgtgacag cctgcgggag cagacgctca gcaagcagac ctaccacaag gaggcccgca
     4321 acagctggga ctcgcctgcc tttagcaacg atgtcatccg caagaggcac taccgcatcg
     4381 gcctgaacct cttcaacaag aagcctgaga agggagtcca gtacctcatc gagcgtggct
     4441 ttgtgcccga cacgcccgtc ggggtggccc acttcctgct gcagcgcaag ggcctcagcc
     4501 ggcagatgat cggcgagttc ctgggcaacc ggcagaagca gttcaaccgt gacgtgctcg
     4561 actgcgtcgt ggacgagatg gacttctcta ccatggagct ggatgaggcc ctcaggaaat
     4621 tccaggcgca catccgtgtc caaggggagg ctcagaaagt ggagcggctc atagaggcgt
     4681 tcagccagcg ctactgcatc tgcaaccctg gggtggtgcg gcaattccgg aacccagaca
     4741 ccattttcat cctggccttc gccatcatcc tgctgaacac cgacatgtac agccccaatg
     4801 tcaagcccga gcggaaaatg aagctagagg acttcatcaa gaacctccga ggtgtggacg
     4861 atggtgagga cattccccgt gagatgctga tggggatcta tgaacggatc cgtaagcgag
     4921 agctaaagac caatgaggac catgtgtccc aggtgcagaa ggtggagaag ctcattgtgg
     4981 ggaaaaagcc gatcggatcc ctgcatcccg ggctcggctg tgtgctctct ctgccccacc
     5041 gtcggttggt ctgctactgc cggctctttg aggttccaga cccaaacaag ccccagaaac
     5101 tcggactaca ccagcgagaa atcttcctgt tcaacgacct cctggtggtc accaagatct
     5161 tccagaagaa gaagaactcg gtgacgtaca gcttccgaca gtccttctcc ttgtacggca
     5221 tgcaggtcct gctcttcgag aaccagtact accccaatgg catccggctc acctcgtctg
     5281 tccccggagc agatatcaaa gtgttaataa acttcaacgc ccccaaccct caagaccgga
     5341 agaaattcac cgatgacctg cgggagtcca ttgcggaagt ccaagagatg gagaagcaca
     5401 ggatagagtc ggagctcgag aagcagaaag gcgtcgtgcg gcccagcatg tcccagtgct
     5461 ctagcctcaa aaaggagtcg ggcaacggaa cactgagccg ggcctgcctg gacgacagct
     5521 atgccagcgg tgagggcctc aagcgcagcg ccctcagcag ctccctgcgg gacctctcgg
     5581 aagccggggt ccatcattag gaattctgca gtcgacggta ccgcgggccc gggatccacc
     5641 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5701 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5761 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5821 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5881 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5941 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6001 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6061 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6121 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6181 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6241 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6301 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6361 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     6421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6661 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7021 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     7081 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7141 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7201 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     7261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9901 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     9961 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
    10021 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
    10081 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
    10141 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
    10201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10321 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10381 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10441 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10501 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10561 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10621 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10681 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10741 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10801 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    10861 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    10921 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    10981 cagagaga
//
