LOCUS       sequence-318918-       13414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector containing PIK3C2B tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pLEX303 GFP-PIK3C2B
FEATURES             Location/Qualifiers
     source          1..13414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        377..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     531..551
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        625..643
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(685..687,688..690,691..1401)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(685..687,688..690,691..1401)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(730..751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(991..1010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1338..1359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3390..3399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6369..6386
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6429..7017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6482..6502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6900..6911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7089..7322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7400..7521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7437..7456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7491..7510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7561..7696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7717..7735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7745..7762)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7745..7761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7754..7776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7903..8358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7990..8009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8200..8221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8384..8488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8489..8557,8558..9349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8707..8726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9520..10108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10009..10028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10262..10279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10396..10417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10432..10437,10438..10455,10456..10462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10470..10486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10475..10497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10494..10510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10494..10510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10529..10549
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10531..10549
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10577..10803
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10804..10984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11031..11156
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11649..11882
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12067..12111
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12286..12786
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     12705..12723
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             12798..13193
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    13253..13370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgagggggcc cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      541 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      601 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
      661 gcgtttaaac ttaagcttgg taccatggtg agcaagggcg aggagctgtt caccggggtg
      721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      781 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      841 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      901 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      961 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1021 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1081 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1141 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1201 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1261 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1321 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1381 ggcatggacg agctgtacaa gggatccgaa ttcatgtctt cgactcaggg caatggggaa
     1441 cactggaagt ccctggagtc agtgggcatc agccgcaaag agctagcgat ggccgaagcc
     1501 ctgcagatgg agtatgatgc cctgtcccgg ctccgacatg acaaggagga gaacagagcc
     1561 aagcagaacg cagacccctc tctcatcagc tgggatgagt ctggggtaga cttttacagc
     1621 aagccagcag gaaggtggac cgacctcaag ctgttacgcg gtctctctgg ctctgatcct
     1681 acccttaact acaactcact atccccactg gaagggccgc ccaaccactc tacctcccaa
     1741 gggccacagc ctggctcaga tccctggccc aaaggctccc tgtctggaga ctatctctac
     1801 atttttgatg gttcagatgg gggagtctct tcgtccccag gaccagggga catagagggc
     1861 tcttgcaaga aactatcccc acctcctctg cctccccgag cttctatctg ggatacccct
     1921 cccctgcctc ccagaaaggg gtccccctca tcctccaaga tctcccagcc cagtgacatc
     1981 aacactttct ctttggtcga acaattgcca ggcaaactgc tagagcatcg gatcctagaa
     2041 gaggaagagg tgctgggagg tgggggtcag gggcgcctac tggggtctgt ggactatgat
     2101 ggtatcaatg atgcaattac taggctcaac ttgaaatcga cctatgatgc ggagatgttg
     2161 cgggatgcca ccaggggctg gaaggagggc cgagggccgc tggacttcag caaagacacc
     2221 tctggaaaac ccgtggccag gagcaagact atgccccctc aggtgccccc ccgcacctat
     2281 gcctcccgct atggcaaccg aaagaatgcg acgcctggca agaaccgccg gatttctgca
     2341 gccccggtgg gctcccggcc ccacactgtt gccaatggcc atgagttgtt tgaggtctca
     2401 gaagagagag atgaggaggt tgctgcattt tgccacatgc tggatatcct tcgatctggc
     2461 tctgacatcc aagactactt cctcactggc tatgtctgga gtgctgtcac ccctagccca
     2521 gagcacctcg gggatgaggt caacctgaag gtgactgtgt tgtgtgacag gcttcaagag
     2581 gcactcactt tcacctgcaa ctgttcctcc actgtagact tgcttatcta ccagaccctg
     2641 tgctacaccc atgatgacct gaggaatgtg gacgtgggtg actttgtgct aaagccctgc
     2701 gggctggagg agttcctgca gaacaagcat gccttgggca gtcatgagta catccaatac
     2761 tgccgcaagt ttgacattga cattcggcta cagctgatgg agcagaaggt tgtgcgcagt
     2821 gacctggccc ggacggtgaa tgatgaccag agcccctcca ccttgaacta cctcgtccat
     2881 ctccaagaga ggcctgtcaa gcagaccatc agcaggcagg ccctgagtct tctgttcgac
     2941 acttaccaca atgaggtgga tgccttcctg ctggctgatg gagacttccc actgaaggct
     3001 gacagggtgg tccagtccgt caaggccatc tgcaacgccc tggccgccgt ggaaacccct
     3061 gagatcacca gtgctctcaa ccagctgccc ccctgcccct cccgcatgca gcctaaaatt
     3121 cagaaggatc ccagtgtctt ggctgtgagg gaaaaccgag agaaggtcgt ggaagccctg
     3181 accgctgcca tcttggacct ggtggagctg tactgcaaca cattcaacgc agacttccag
     3241 acggcagtgc ccgggagccg caagcatgac ctggtccagg aggcctgcca tttcgccagg
     3301 tccctggcct ttactgtcta tgccacccac cgcatcccca tcatctgggc taccagttac
     3361 gaggacttct acctctcctg ctccctcagc catggcggca aggagctgtg cagccccctg
     3421 cagacccgaa gagctcactt ctccaagtac ctcttccacc tcatcgtctg ggaccagcag
     3481 atctgcttcc cagtgcaggt gaaccggctg cctcgggaga cactgctgtg tgccactctc
     3541 tatgctctgc ccatcccccc accggggagc tcctcagagg ccaataagca gcggcgggtg
     3601 cctgaagccc tgggctgggt cactacccca ctcttcaact tcaggcaggt cctgacctgt
     3661 ggccggaagc ttctgggttt gtggccagca acacaggaaa atcccagcgc ccgttggagt
     3721 gcacctaatt tccaccagcc agacagtgtc atcctgcaga ttgacttccc cacctcggcc
     3781 tttgacatca agttcaccag cccccctgga gacaagttca gcccccgcta tgagtttggc
     3841 agcctccggg aagaagacca gcgcaagctt aaagacatca tgcagaaaga gtccttgtac
     3901 tggctcactg atgctgacaa gaagcgcctg tgggagaagc gatattactg ccactcggag
     3961 gtgagctcgc tccccctggt gctcgccagc gcccccagct gggagtgggc ttgcctgcct
     4021 gacatctatg ttctcctgaa gcagtggacc cacatgaacc accaggatgc cctggggctc
     4081 ctgcatgcca ccttcccgga ccaggaggtg cgtcgtatgg ctgtgcagtg gattggctca
     4141 ctctcagatg ctgagctgct agactacctg ccccagctgg tacaggccct gaagtatgaa
     4201 tgctacctgg acagcccgtt ggtgcgcttc ctcctgaaac gagctgtgtc tgacttgaga
     4261 gtgactcact acttcttctg gttactgaag gacggcctca aggactctca gttcagcatc
     4321 cgctaccagt atctgctggc agccttactg tgctgctgtg gcaaggggct gagagaagag
     4381 tttaaccgcc agtgctggct tgtcaatgcc ctggccaaac tggcccagca ggtccgggag
     4441 gcagccccat ctgcaaggca gggaatcctc cgcacgggcc tggaggaggt gaagcagttc
     4501 tttgccctca atggctcgtg ccgcttgcca ctcagcccca gtctgctggt taagggaatt
     4561 gtgcccaggg actgttccta cttcaactcc aatgctgtcc ccctcaaact ctccttccaa
     4621 aatgtggatc ccctgggtga gaacatccgt gtcatcttca agtgtgggga cgaccttcgc
     4681 caggacatgc taacgctgca gatgattcgc atcatgagca agatctgggt ccaggagggg
     4741 ctggacatgc gcatggtcat cttccgctgc ttctccaccg gccggggcag agggatggtg
     4801 gagatgatcc ctaatgctga gaccctgcgt aagatccagg tggagcatgg ggtgaccggc
     4861 tcgttcaagg accggcccct ggcagactgg ctgcagaaac acaaccctgg ggaggacgag
     4921 tatgagaagg ctgtggagaa ctttatctac tcctgcgctg gctgctgcgt ggccacgtac
     4981 gtcttgggca tctgtgaccg acataacgac aacatcatgc tgaagaccac tggtcacatg
     5041 ttccacattg attttggccg cttcctgggc catgcccaga tgtttggcaa catcaagcgg
     5101 gaccgtgccc cctttgtctt cacctcggac atggcgtatg tcatcaacgg gggtgacaag
     5161 ccttccagcc gcttccatga ttttgttgac ctttgctgcc aagcctacaa cctcattcgc
     5221 aagcacaccc acctcttcct caaccttctg ggcctgatgt tgtcctgtgg gatccctgaa
     5281 ctctcagacc tggaggacct caagtatgtg tacgatgccc tgaggcctca ggatacagag
     5341 gccaatgcca ctacctactt cactaggttg attgagtcca gcctgggcag tgtagccaca
     5401 aagctcaatt ttttcatcca taatctggct cagatgaagt tcacgggctc agatgaccgg
     5461 ctgaccctct cctttgcctc ccgaacacac actctcaaga gctctggccg aatcagtgat
     5521 gttttcctct gccgccatga gaagatcttc caccccaaca aaggctatat atatgtggta
     5581 aaggtgatgc gagagaacac tcacgaggcc acctacatcc agcggacctt tgaggagttc
     5641 caggaattac acaataagtt gcggctgctc ttcccttctt cccacttgcc cagcttccct
     5701 agtcgcttcg tgatcggccg ctcccgggga gaggcggtgg ccgagcggcg gagggaggag
     5761 ctaaacggtt acatctggca cttgatccac gcacctcccg aggtggccga gtgtgatttg
     5821 gtgtacacct tcttccaccc actgccccgg gatgagaagg ctatgggcac cagcccagct
     5881 cctaagtcct cagatggcac atgggcccgg cccgtcggaa aggtgggagg ggaggtgaag
     5941 ctgtctatct cctacaaaaa caataaactc ttcatcatgg tgatgcatat tcggggcttg
     6001 caactgctcc aggatggaaa tgaccctgac ccctatgtga aaatttacct ccttcctgac
     6061 cctcagaaaa ccactaagag gaaaaccaaa gtggcccgga aaacctgcaa tcctacctac
     6121 aatgagatgt tggtatatga tgggatcccc aagggtgacc tgcagcagcg ggagctccag
     6181 ctgagcgtgc tgagtgagca gggattctgg gagaacgtcc tcctcggtga ggtgaacatc
     6241 cgcctgcgag agctggacct ggctcaggag aagaccggct ggttcgccct gggatctcga
     6301 agtcatggca ccttgtaatc tagccctgca ggggcggagg gggtggagtt aactgtacat
     6361 taattaacca ccaccaccac caccactaag gatcccgtac gtgatcaccg gtggcgcgtt
     6421 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6481 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6541 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6601 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6661 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6721 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6781 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6841 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6901 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6961 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     7021 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7081 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7381 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7501 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7561 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7621 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7681 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     7741 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7921 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7981 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8041 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8101 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8161 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8221 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8281 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8341 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9661 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10141 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10201 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10261 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10321 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10381 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10441 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10501 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    10561 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
    10621 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
    10681 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
    10741 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
    10801 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10861 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10921 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10981 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    11041 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
    11101 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
    11161 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
    11221 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
    11281 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
    11341 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
    11401 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
    11461 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
    11521 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    11581 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    11641 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    11701 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    11761 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    11821 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    11881 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    11941 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    12001 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    12061 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    12121 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    12181 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    12241 tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa
    12301 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
    12361 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
    12421 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
    12481 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
    12541 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
    12601 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
    12661 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
    12721 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
    12781 ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
    12841 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
    12901 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
    12961 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
    13021 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
    13081 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
    13141 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
    13201 tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
    13261 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    13321 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    13381 acagggacag cagagatcca ctttggcgcc ggct
//
