LOCUS       sequence-318919-       11564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 chimera.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX303-GFP-IQSEC1 v4N/v1C-WT
FEATURES             Location/Qualifiers
     source          1..11564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     604..624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(698..700,701..703,704..1414)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(698..700,701..703,704..1414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(743..764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1004..1023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1351..1372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4241..4261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    4652..5240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4705..4725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5123..5134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5312..5545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5623..5744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5660..5679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5714..5733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5784..5919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5939..5958)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5940..5958)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5968..5985)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5968..5984)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5999)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6126..6581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6213..6232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6423..6444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6607..6711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6712..6780,6781..7572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6930..6949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7743..8331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8232..8251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8485..8502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8619..8640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8655..8660,8661..8678,8679..8685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8693..8709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8698..8720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8717..8733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8717..8733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8752..8772
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8754..8772
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8800..9026
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9027..9207
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9254..9379
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9872..10105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10290..10334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10509..11009
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10928..10946
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             11021..11416
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(11476..11564,1..29)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
       61 tttggcgccg gctcgagggg gcccggggtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      661 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      721 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      781 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      841 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      901 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      961 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1021 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1081 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1141 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1201 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1261 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1321 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1381 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
     1441 ttacatgcta gagcgaaagt atggggggcg cctggtaacc cgccatgcgg cccgcaccat
     1501 ccagacggcg tttcgccaat atcaaatgaa taaaaacttc gagcgcttgc gcagctccat
     1561 gtcagagaac cgcatgtcac gccggattgt gctgtccaac atgaggatgc agttctcctt
     1621 tgaggggcct gagaaagtgc acagctccta cttcgagggg aagcaggtct cagtgactaa
     1681 cgacggctcc cagctgggag ccctggtgtc ccctgagtgt ggtgacctca gcgagcccac
     1741 caccctcaag tctccggccc cctccagtga ctttgcggac gccatcaccg agctggagga
     1801 cgccttctct aggcaagtga aatcactggc cgagtccatc gacgatgccc tcaactgccg
     1861 cagcctgcac actgaggagg caccggccct ggatgcggcg cgggcccggg acaccgaacc
     1921 ccagacagcc ctgcacggca tggaccaccg caaactggac gagatgacgg cctcgtacag
     1981 tgatgtcacc ctgtacatcg atgaggagga gctgtcgccc cctctgcccc tctcgcaggc
     2041 aggggaccgg ccgtccagca ccgagtcgga cctgcggcta cgggctgggg gcgcagcccc
     2101 agactactgg gccctggccc acaaagagga caaggctgac acggacacga gctgccggag
     2161 cacgccgtcg ctggagcggc aggagcagcg gctgcgggtg gagcatctgc cgctgctcac
     2221 catcgagcca cccagcgaca gctctgtgga ccttagtgac cgctcggagc gggggtcact
     2281 caagaggcag agtgcttacg agcgcagcct tggcgggcag cagggcagtc ccaagcatgg
     2341 tccccacagc ggcgccccca agagcctccc ccgggaggag cctgagttgc ggccccggcc
     2401 ccccaggccc ctggacagcc acttggccat caatggctca gccaaccggc agagcaagtc
     2461 tgagtcggac tactcagacg gtgacaatga cagcatcaac agcacgtcca actccaacga
     2521 taccatcaac tgcagctccg agtcatcgtc ccgtgacagc ctgcgggagc agacgctcag
     2581 caagcagacc taccacaagg aggcccgcaa cagctgggac tcgcctgcct ttagcaacga
     2641 tgtcatccgc aagaggcact accgcatcgg cctgaacctc ttcaacaaga agcctgagaa
     2701 gggagtccag tacctcatcg agcgtggctt tgtgcccgac acgcccgtcg gggtggccca
     2761 cttcctgctg cagcgcaagg gcctcagccg gcagatgatc ggcgagttcc tgggcaaccg
     2821 gcagaagcag ttcaaccgtg acgtgctcga ctgcgtcgtg gacgagatgg acttctctac
     2881 catggagctg gatgaggccc tcaggaaatt ccaggcgcac atccgtgtcc aaggggaggc
     2941 tcagaaagtg gagcggctca tagaggcgtt cagccagcgc tactgcatct gcaaccctgg
     3001 ggtggtgcgg caattccgga acccagacac cattttcatc ctggccttcg ccatcatcct
     3061 gctgaacacc gacatgtaca gccccaatgt caagcccgag cggaaaatga agctagagga
     3121 cttcatcaag aacctccgag gtgtggacga tggtgaggac attccccgtg agatgctgat
     3181 ggggatctat gaacggatcc gtaagcgaga gctaaagacc aatgaggacc atgtgtccca
     3241 ggtgcagaag gtggagaagc tcattgtggg gaaaaagccg atcggatccc tgcatcccgg
     3301 gctcggctgt gtgctctctc tgccccaccg tcggttggtc tgctactgcc ggctctttga
     3361 ggttccagac ccaaacaagc cccagaaact cggactacac cagcgagaaa tcttcctgtt
     3421 caacgacctc ctggtggtca ccaagatctt ccagaagaag aagaactcgg tgacgtacag
     3481 cttccgacag tccttctcct tgtacggcat gcaggtcctg ctcttcgaga accagtacta
     3541 ccccaatggc atccggctca cctcgtctgt ccccggagca gatatcaagg tgctgatcaa
     3601 cttcaacgcc cccaaccccc aggaccggaa gaagttcacc gacgacctgc gcgagtctat
     3661 cgccgaggtg caggaaatgg aaaagcaccg gatcgagagc gagctggaaa agcagaaagg
     3721 cgtggtgcgc cccagcatga gccagtgcag cagcctgaag aaagagagcg gcaacggcac
     3781 cctgagccgg gcctgcctgg atgatagcta cgccagcggc gagggcctga agagaagcgc
     3841 cctgagcagc tccctgcggg atctgagcga ggccggcaag cggggcagaa gaagcagcgc
     3901 cggcagcctg gaaagcaacg tggaaggcag catcatcagc agcccccaca tgcggcggag
     3961 agccaccagc accagagagt gccccagcag accccaccag accatgccca actcctccag
     4021 cctgctgggc agcctgttcg gcagcaagag aggcaagcca ccaccccagg cccatctgcc
     4081 atctgcccct gccctgcctc ctccacaccc tcccgtggtg ctgcctcatc tgcagcacag
     4141 cgtggccgga caccacctgg gacctccaga aggactgccc caggccgcca tgcacggcca
     4201 tcacacccag tactgccaca tgcagaaccc ccctccctac caccaccatc accatcatca
     4261 ccccccccag cacatccagc acgcccacca gtatcaccac ggccctcacg gcggacaccc
     4321 tgcctatggc gcccacgccc atggacaccc tccactgcct agcgcccatg tgggacacac
     4381 agtgcaccac cacggacagc ctcccgcccc tccacctcct accagcagca aggccaagcc
     4441 cagcggcatc agcaccattg tgtgagaatt ctgcagtcga cggtaccgcg ggcccgggat
     4501 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     4561 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4621 ttgttgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     4681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5041 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5221 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     5281 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5341 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5401 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5461 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5521 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     5581 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5641 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5701 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5761 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5821 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5881 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5941 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6001 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6061 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6121 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6601 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6661 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6721 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6781 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6841 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6901 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6961 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7021 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7081 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7141 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7201 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7261 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7321 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7381 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7441 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7501 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7561 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7621 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7681 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7741 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7801 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7861 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7921 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7981 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8041 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8101 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8161 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8221 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8281 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8341 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8401 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8461 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8521 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8581 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8641 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8701 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     8761 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     8821 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     8881 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     8941 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     9001 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     9061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9181 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9241 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9301 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9361 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9421 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9481 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9541 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9601 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9661 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9721 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9781 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9841 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9901 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9961 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10021 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10081 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10141 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10201 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10261 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10321 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10381 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10441 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10501 ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
    10561 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
    10621 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
    10681 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
    10741 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
    10801 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
    10861 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
    10921 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
    10981 ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca
    11041 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
    11101 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
    11161 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
    11221 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
    11281 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
    11341 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
    11401 ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc
    11461 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    11521 aatagtagac ataatagcaa cagacataca aactaaagaa ttac
//
