LOCUS       sequence-318920-       11354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 chimera.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX303-GFP-IQSEC1 v2N/v4C-WT
FEATURES             Location/Qualifiers
     source          1..11354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    484..609
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             656..836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(837..1063)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1091..1111)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1091..1109)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1130..1146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1130..1146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1143..1165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1154..1170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1178..1184,1185..1202,1203..1208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1223..1244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1361..1378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1532..2120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1612..1631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2291..3082,3083..3151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2914..2933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3152..3256)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3282..3737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3419..3440)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3631..3650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3864..3886
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3878..3895
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3879..3895
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3905..3924
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3905..3923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3944..4079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4119..4240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4130..4149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4184..4203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4318..4551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4623..5211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4729..4740
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5138..5158
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(8239..8949,8950..8952,8953..8955))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8239..8949,8950..8952,8953..8955))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8281..8302)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8630..8649
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8889..8910
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8952..8961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9000..9203)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9029..9049)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9204..9507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    9624..9741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9801..10196)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(10208..10708)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10271..10289)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(10883..10927)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11112..11345
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
       61 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
      121 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
      181 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
      241 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
      301 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
      361 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
      421 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
      481 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
      541 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
      601 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
      661 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      721 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      781 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
      841 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
      901 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
      961 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
     1021 gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct tgcagctcca
     1081 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     1141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     3301 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3361 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3421 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3481 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3541 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3601 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3661 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3721 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3781 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     3841 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
     3901 cgcgtaatac gactcactat agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct
     3961 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     4021 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     4081 ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg
     4141 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4201 tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc
     4261 atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta tacgtactgc
     4321 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     4381 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     4441 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     4501 tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct
     4561 tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt cttaaagtcg
     4621 acgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     4681 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     4741 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     4801 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     4861 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     4921 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     4981 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     5041 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     5101 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     5161 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgactta
     5221 acgcgccacc ggttagcaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     5281 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     5341 atcagttatc tagatccggt ggatcccggg cccgcggtac cgtcgactgc agaattccta
     5401 atgatggacc ccggcttccg agaggtcccg cagggagctg ctgagggcgc tgcgcttgag
     5461 gccctcaccg ctggcatagc tgtcgtccag gcaggcccgg ctcagtgttc cgttgcccga
     5521 ctcctttttg aggctagagc actgggacat gctgggccgc acgacgcctt tctgcttctc
     5581 gagctccgac tctatcctgt gcttctccat ctcttggact tccgcaatgg actcccgcag
     5641 gtcatcggtg aatttcttcc ggtcttgagg gttgggggcg ttgaagttta ttaacacttt
     5701 gatatctgct ccggggacag acgaggtgag ccggatgcca ttggggtagt actggttctc
     5761 gaagagcagg acctgcatgc cgtacaagga gaaggactgt cggaagctgt acgtcaccga
     5821 gttcttcttc ttctggaaga tcttggtgac caccaggagg tcgttgaaca ggaagatttc
     5881 tcgctggtgt agtccgagtt tctggggctt gtttgggtct ggaacctcaa agagccggca
     5941 gtagcagacc aaccgacggt ggggcagaga gagcacacag ccgagcccgg gatgcaggga
     6001 tccgatcggc tttttcccca caatgagctt ctccaccttc tgcacctggg acacatggtc
     6061 ctcattggtc tttagctctc gcttacggat ccgttcatag atccccatca gcatctcacg
     6121 gggaatgtcc tcaccatcgt ccacacctcg gaggttcttg atgaagtcct ctagcttcat
     6181 tttccgctcg ggcttgacat tggggctgta catgtcggtg ttcagcagga tgatggcgaa
     6241 ggccaggatg aaaatggtgt ctgggttccg gaattgccgc accaccccag ggttgcagat
     6301 gcagtagcgc tggctgaacg cctctatgag ccgctccact ttctgagcct ccccttggac
     6361 acggatgtgc gcctggaatt tcctgagggc ctcatccagc tccatggtag agaagtccat
     6421 ctcgtccacg acgcagtcga gcacgtcacg gttgaactgc ttctgccggt tgcccaggaa
     6481 ctcgccgatc atctgccggc tgaggccctt gcgctgcagc aggaagtggg ccaccccgac
     6541 gggcgtgtcg ggcacaaagc cacgctcgat gaggtactgg actcccttct caggcttctt
     6601 gttgaagagg ttcaggccga tgcggtagtg cctcttgcgg atgacatcgt tgctaaaggc
     6661 aggcgagtcc cagctgttgc gggcctcctt gtggtaggtc tgcttgctga gcgtctgctc
     6721 ccgcaggctg tcacgggacg atgactcgga gctgcagttg atggtatcgt tggagttgga
     6781 cgtgctgttg atgctgtcat tgtcaccgtc tgagtagtcc gactcagact tgctctgccg
     6841 gttggctgag ccattgatgg ccaagtggct gtccaggggc ctggggggcc ggggccgcaa
     6901 ctcaggctcc tcccggggga ggctcttggg ggcgccgctg tggggaccat gcttgggact
     6961 gccctgctgc ccgccaaggc tgcgctcgta agcactctgc ctcttgagtg acccccgctc
     7021 cgagcggtca ctaaggtcca cagagctgtc gctgggtggc tcgatggtga gcagcggcag
     7081 atgctccacc cgcagccgct gctcctgccg ctccagcgac ggcgtgctcc ggcagctcgt
     7141 gtccgtgtca gccttgtcct ctttgtgggc cagggcccag tagtctgggg ctgcgccccc
     7201 agcccgtagc cgcaggtccg actcggtgct ggacggccgg tcccctgcct gcgagagggg
     7261 cagagggggc gacagctcct cctcatcgat gtacagggtg acatcactgt acgaggccgt
     7321 catctcgtcc agtttgcggt ggtccatgcc gtgcagggct gtctggggtt cggtgtcccg
     7381 ggcccgcgcc gcatccaggg ccggtgcctc ctcagtgtgc aggctgcggc agttgagggc
     7441 atcgtcgatg gactcggcca gtgatttcac ttgcctagag aaggcgtcct ccagctcggt
     7501 gatggcgtcc gcaaagtcac tggagggggc cggagacttg agggtggtgg gctcgctgag
     7561 gtcaccacac tcaggggaca ccagggctcc cagctgggag ccgtcgttag tcactgagac
     7621 ctgcttcccc tcgaagtagg agctgtgcac tttctcaggc ccctcaaagg agaactgcat
     7681 cctcatgttg gacagcacaa tccggcgtga catgcggttc tctgacatgg agctgcgcaa
     7741 gcgctcgaag tttttattca tttgatattg gcgaaacgcc gtctggatgg tgcgggccgc
     7801 atggcgggtt accaggcgcc ccccatactt tcgctctagc atctccacct gcttgtcctg
     7861 caggtccgag gagagctcat agctctcgga gagtgagcgt gagcgcttga tggcctcctc
     7921 ctcagcctgc ttgcgcagga tggaggtcga gtgctgcagc ttgggcctcc gcgtgcgctg
     7981 ctgttgcccc ggcggccccg agtacagccc ataggctccc actgacgtgt gctcgtagtg
     8041 atccgggctc aggctggaac cgggcaccaa ggggccctgg gggtaggctg aggggctgtc
     8101 cagggatgtg ccagtctcac tgctgggggc ctcgccctcg acgccgctgt caagctctag
     8161 ctccagaagg gactgggtcc tctccgagtt gcagtgcagg caccacatgt aagcttgagc
     8221 tcgagatctg agtccggact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     8281 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     8341 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     8401 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     8461 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     8521 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     8581 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     8641 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     8701 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     8761 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     8821 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     8881 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     8941 gcccttgctc accatggtgg cgaccggtag cgctagcgga tctgacggtt cactaaacca
     9001 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     9061 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     9121 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     9181 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     9241 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     9301 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     9361 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     9421 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     9481 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     9541 cccgtaattg attactatta ataaccccgg gccccctcga gccggcgcca aagtggatct
     9601 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     9661 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     9721 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctcaa gatctagaat
     9781 tcttactact gcagctgcag gccctcccac acataaccag agggcagcaa ttcacgaatc
     9841 ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga
     9901 tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg
     9961 atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg
    10021 agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg
    10081 tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt
    10141 gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg
    10201 gtaccccctg gggagagagg tcggtgattc ggtcaacgag ggagccgact gccgacgtgc
    10261 gctccggagg cttgcagaat gcggaacacc gcgcgggcag gaacagggcc cacactaccg
    10321 ccccacaccc cgcctcccgc accgcccctt cccggccgct gctctcggcg cgccctgctg
    10381 agcagccgct attggccaca gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct
    10441 gtccgtctgc gagggtacta gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg
    10501 cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg aagcgtcgcc ggggggccca
    10561 caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc
    10621 cagggtcggc gccgctgcgt ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa
    10681 acccagggct gccttggaaa aggcgcaagg gtcccctcgg ggttgggagg tgggtctgaa
    10741 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta
    10801 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat
    10861 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt
    10921 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat
    10981 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt
    11041 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac
    11101 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct
    11161 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc
    11221 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca
    11281 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag
    11341 ctcctattcc cact
//
