LOCUS       sequence-318925-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 3 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG019 (AVAST type 3)
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7280..7327
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7547..8206)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8121..8140
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8207..8309)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8701..8718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8835..9380)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8915..8934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
       61 aatgagtgag ctaacttaca ttaattgcgt tgcgcaacaa ttttttgcca taagacgctt
      121 tcctgaaact cttctcattc tcagcaggaa agcgttctct tctcaatact ctctggttat
      181 agagtattaa aaaataagga gttataatcc ttgtagccca actgacataa ggacgatgct
      241 caatgtctga cagcctgctt gttcgcacca gtagagatgg cgatcagttt cattatcttt
      301 gggcggctcg ccgcgccctt cgactactgg aacctcagtc aactcttgtt gccctgacca
      361 ttgaaggggc atcaacgacg gaaatgggct ctcagccagt ggttgaggat ggggaggagc
      421 tgattgatat tgctgaatat tacggcagta acgagctcgc aacagcaaca actgttcgtt
      481 atatgcagct aaagcattca acaatgcact cagatactcc atttccccct agtgggttac
      541 aaaaaaccat cgaaggtttt gcaacccgtt ataaggcact tatacaaaaa ataccggtag
      601 aaacgttacg cactaaactc gagttctggt ttgtgacgaa ccgtccagtc agtagcagct
      661 tcagtgaagc gatcaatgat gccgcgaacc aacacgttac acgccatcca catgatctgg
      721 cgaaacttga gaaatttacc gggcttcaag gcgctgagtt atcgatattc tgccagcttt
      781 tacatataga aggtcagcag gacgatttat ggagtcagcg gaatatcctg ctaagagaat
      841 cagcgggata tctccccgac ctggatactg aagcccctct gaaattaaaa gagctggtta
      901 acagaaaagc gttaaccgaa agcgccgcaa atccttccat taccagaatg gatgtgttgc
      961 gtgctttggg ggtggatgaa acagatcttt ttcctgcgcc ctgtcgtatt gaaagaatag
     1021 aaaattccgt ctcaagaact caagaggcga cgctggttca acgtgttgtt gaagcattcg
     1081 gcgcacctgt gatcatccat gccgatgccg gtgtggggaa atcaattttc tctactcata
     1141 tagaggagca tcttcccact ggttctgtta gcatcttata tgactgtttc ggactgggtc
     1201 agtaccgtaa cgcgtcttcc taccgccacc accatcgtac agcattggtt cagatggcta
     1261 atgaaatggc atctcgtggt ctctgtcatc cattgatccc aaatgctggt actggcatat
     1321 cccagtatat gcgtgcgttt ctgcatcgcc tttctcagag catttcaata ctccgggcct
     1381 ctgagccctt ggccgtattg tgtattatta ttgatgctgc ggacaatgca cagatggcgg
     1441 cggaagaaat cggtgaaacg cgttctttta tcaaagattt aattagagaa aagcttcctg
     1501 atggagtctg ccttgttgca ctttgccgac cttatagacg ggaattactt gatccacctc
     1561 ctgaagcact cacattatcc ctacaaactt ttaatcgcga tgagacagcc gctcatcttc
     1621 accaaaaatt tccagatgcc agcgaaagtg atgttgacga gttccatcgt ctaagctctt
     1681 gcaacccccg ggttcaggct ctgtcattat cacaaaatct tccactgaac gacacattga
     1741 gacttttggg gccaaatccc aaaacggtag aagatactat tggtgaagtg ctggaaaaat
     1801 ccattgctcg cttacgtgat acagccggaa tatctgaacg tgctcaaatt gatacgattt
     1861 gttccgcact ggcaatattg cgtccattaa ttccattatc tgtgctatct gccatttccg
     1921 gagtagctgg ttctgctatt aaaagtttcg cacttgatct gggacgcccg ttaatcgtta
     1981 gtggcgagac tattcagttc tttgatgaac cggccgaaac atggtttcag aggcgcttta
     2041 ggccatcggc cgctgatctg catcagttta ttactaaact gagaccacta acaaaagata
     2101 gttcctatgc agcatcagtt ttacctgcat tgatgctgga aggaaaccag ctttctgaac
     2161 tgatcgagct agcgatatcc tcacaagctc tgcctgaaac cagcgcggtt gaacgcaggg
     2221 acatagaact tcaaagatta cagtttgcgt taaaagcagc cttacgcaca ggtcgatacc
     2281 aggatgcggc taaactggca ctgaaagctg gtggagaatg cgcgggtgac aacaggcaaa
     2341 gagtcctgct gagggacaat atcgatctgg cagcaaaatt tgtgggaagc aacggcgttc
     2401 aggaactggt ttcccgtaac gcatttccag atactggctg gcctggctcc agaaatgctt
     2461 attatgccgc aatactttcc gaatatcctg aactctcagg agaggcccgc agtcgccttc
     2521 gactcaccat ggagtggtta acaaactgga gtcaattacc agatgatgag cggagcaggc
     2581 aaaatgttac cgatcaggac agagcggtaa tgctcattgc ctgcctgaat attcatggcg
     2641 cggaagcggc agcaagggag ctcagaaggt ggcggcctcg aaaactatct tttgacgctg
     2701 gaaaaattgt tgccatgcag ttactggccc acgcccgtta tgatgaactt gatcagttgg
     2761 ctattgcggc tggaaacgat atcagcctgg ttatgggaat tgtactggaa gcaagaaaac
     2821 ttcaccgtcc agtcgctgaa caagcaatca gaagaacctg gcgcttgtta aaaagtcagc
     2881 gagtcagcat taaagacaga aaccacgcta ataaccagac aatagcagca atcactggca
     2941 tggttgaaat ggcgcttatc caatctgttt gtactgaatc agaaagcatc cagttgttgg
     3001 atcgttattt accaaaggtt cccccctatg ctctgacttc tgagtatagt aaagaaagag
     3061 ttgcttacgt ccgggcatat gctctgcagg caaacctgat gggctctcaa ttagcgctta
     3121 gcgatttagc ctccacagag gttaaaaaag aacttatggc tgaaaaacgc cacggcgaat
     3181 ctgatgacct gcgtcaactg aagcagtaca gcggagtatt aatcccttgg tataatttat
     3241 gggccaaagt aattcttggt aaaacaagga aagcagactt agaaagtgag ctaagtgata
     3301 ctcaaaaaga atcgacggct attaaaggtc attcttactc tgagcattca ttatcatcaa
     3361 atgagatcgc aaatgtatgg tttgatattc tgatcgaagc aggtaatgta tcaaaagacg
     3421 atgtggaaaa catcatcaaa tggagtcagc ataaagggaa tagagtattc acaccaacgc
     3481 ttcaccgttt cagttctgta tgtgcagaga tttcagggct tggagagctt tcatatcact
     3541 tcgcagaact tgccttatct ttatggaggg atgagcactc tgatgctcag atcaaagctg
     3601 acggctatat agacctttcc cgttcactca tttcacttga tgaaccagaa gctaaagaat
     3661 actttaacca agcgattgaa gttacaaata agttaggcga tgaaaattta agtcgatggg
     3721 aagcgatact tgatcttgct gaatatgttg ctggtaaaac gcaagtccct cctgaaattt
     3781 cctataaact agcccgatgt gcggaactaa ccagagaata tgttgatcgt gataaacatt
     3841 ttgcatggag tgatactgtt gagattttgg ctgagttatg tccatcttca gccctagcaa
     3901 taataagtcg ttggcgtgac cgtacatttg gcaatcatag aagcatactg gcatggacca
     3961 ttgagcatct tgtaaagaaa aataaaatta atgcactcga tgcacttcct ttaatcacat
     4021 ttgagaatga ttggcataaa tgcgacttgc ttgattcagt tttatcctcg tgtactgatg
     4081 acaaagataa gatcatggca ttcgaagtgg tttaccacta tacaaaattt aacgtacaaa
     4141 atatccaaaa tcttaaaaag ctggatgcta tttctacatc attaggtatt gaacacacag
     4201 aactgaaaga aagaatttca ggtctacaac atactgagac ggtttcaaaa aaatccagtc
     4261 tctcatcgaa tgataatgag caaggccatg accaggaatg ggagtccatt tttaaagatt
     4321 gtgatttatc gtctattgat ggtattagtg cagcatacga aaaatttcgt aatgttcctg
     4381 aattctattc caaagaaacc ttcatcaaga aagcaataag ccgagttaag acgggcaaag
     4441 aatgtagttt cattactgcc attggtgcta tatttcactg ggggctttat gattttaaat
     4501 atattcttga atctataccc gacgaatgga catctcgttt aagcattaaa accaccctgg
     4561 caggtttaat aaaagaatat tgccaacgct tctgtatgcg aatcagaaaa agtcgcgttt
     4621 acgagatttt tcccttcagt ctggccagca ggctttctgg tataagtgaa aaagagattt
     4681 tcggtattac cctggaggcc attgcagaat cgccagagcc cgcaaactct gaccgtttat
     4741 ttagccttcc tggccttctt gttagtaaac tggagagtaa tgaagcgtta gatgtattat
     4801 cttatgcctt ggatttattc gacgaggtgc taaaagatga ggatggtgac ggcccatgga
     4861 acgagaaatt atctccgcca actcatgtag aggattcact tgcaggctat atttgggcgc
     4921 ggctgggttc tccggaggcg gaaatgcgct ggcaggcagc acatgcggtt ctggcactat
     4981 gtcgaatgag tcgtacatgc gttatacaag gaattttcca gcacgcaata aatgctacca
     5041 ctttaccttt ttgtgatcgc aatctgccct tttataccct ccatgctcaa ttgtggttga
     5101 tgatcgctgc tgcaagggtt gcgctggatg atggaaaatc gctgattccc aatattggtt
     5161 atttctacca ttatgccact actgatcagc cacatgtatt aatccgtcat tttgctgcca
     5221 gaactttact tgcactgcat gatagcgacc tgatctctat cccagcacaa gaagagaata
     5281 aactccgaaa tataaaccag tctacgactc tccctgtgct tgataaggtt gaagatcata
     5341 gaggtgaaga ttcatatact tttggtatcg actttggccc ttactggcta aaacctctgg
     5401 gacgttgttt cggtgtatct caaaaacagt tagaacctga aatgcttcgc attattcgtg
     5461 atgttcttgg ttttaaaggt agccgcaact gggatgagga tgagcgtaat aaacgacgct
     5521 attatcaaga cagagataat catcacagtc atggttccta tccacgggtc gatgactacc
     5581 atttttactt gtcataccat gcaatgttta tgaccgctgg gcagttatta gcgacaaaac
     5641 cattagttgg tagtgactac gacgatgtcg aggatgtttt ccaggactgg ttaagaagac
     5701 atgatatttc tcggaacgat catcgctggc tcgccgatcg gagagatatt ccccccaaag
     5761 agcgctccag ttggcttaat agcagttctg acaataggga tgaatggcta gcgtcaatct
     5821 ctgaaaatgt atttaacgaa acactatgtc ccagccccgg actattaacg ctatggggac
     5881 gttggtctga cgtttgttca gatcgaaaag aatctattat tgtccattct gcgttagtat
     5941 cgccggagcg atctttatcg ctcctcagag cattacaaac aactaaaaat gtatatgact
     6001 ataaaatccc tgatgctgga gataatcttg aaatagatca cgcacactat cagctaaaag
     6061 gatggattaa agatattgct gaatactgtg gaattgatga gtttgatccc tgggcaggta
     6121 atgtaaggtt tccaatccca gaaccagcct catttatcat tgatgcgatg aaattaacta
     6181 ctgataaaga tcatcgggta tggtattcac cttctgatgt tgaaccggcg atgatttcca
     6241 gtatctgggg ccatctatca ggtaaaaatg atgaggaaaa atcacatggt tataggctat
     6301 gtgcttcaat acacttcata aaatcagcat tagaaacatt caacatggat ctcattttag
     6361 aggttgatgt tgatcgctat tcacggaaca gcagatatga acggaataat gaaaatgagc
     6421 tcgacaatat cccttcaagc actcgactct tcctcttccg acatgacgga accatccaca
     6481 cgctatacgg caattataga aatggggaaa aaactagttg atgagcttga gctaaatgac
     6541 tctgttgata cattaagcag atggatggct catcatatcg cagagctcat ttatgatgct
     6601 gaacattgta cagacgacat cgtccgtaca gctaaacaag cggagattag ggactctatc
     6661 tggtcattct ggtctaacag atacgaattg ccaattggta gcagaccatt tcaggagctc
     6721 gaacctattc taagaacctt aaaaggtctt gatcctgaaa atgagcaacc gagatttttt
     6781 tcaccttacc gagatctaat taatgtagaa aaagaaacca gtgaggtcca aaaatggcta
     6841 accgccgcta aggatattga ttcagcagca aaaatactga ttgattactg tttatcgtta
     6901 gcagcagaaa atgctatcga taaatcccaa gaatgggtgg aattagcaca gaaagctgga
     6961 ttgaacaaag atgttgatct gcttgaaatt cgtatctttc agttacgagg taccccagcc
     7021 aatacagaca atcccaataa tgcacaacgg agaatactgg aaaaaaggca aaaaaggctt
     7081 gaagcttttc tcttattggg ctcccagtta aacgaacaac tcaaatctca gcttgaagcc
     7141 ttaccagcaa ttgaggatga gccaacggat gacgacgaag acttttgata tgacttgctt
     7201 tagcactgga gacggctcac aagacggacc acataatagc ctaacccaag acttttctac
     7261 tagtcctaat gcgcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     7321 ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa
     7381 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac
     7441 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     7501 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     7561 gccactcatc gcaatactgt tgtaattcat ttaacattct gccgacatgg aagccatcac
     7621 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     7681 atttgcccat agtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     7741 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     7801 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     7861 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     7921 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcatcg
     7981 ccatacggaa ctctggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     8041 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     8101 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     8161 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     8221 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     8281 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     8341 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     8401 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     8461 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     8521 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     8581 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     8641 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     8701 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     8761 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     8821 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     8881 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     8941 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     9001 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     9061 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     9121 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     9181 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     9241 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     9301 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     9361 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     9421 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     9481 aattctcatg ttagtcatgc cccgcgccc
//
