LOCUS       sequence-318926-        5753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DUF4297 + HerA defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG025 (DUF4297 + HerA)
FEATURES             Location/Qualifiers
     source          1..5753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3590..3637
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3857..4516)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4431..4450
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4517..4619)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5011..5028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5145..5690)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5225..5244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc
       61 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
      121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg cagtgcgcct tatgtgatta
      181 caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca taaccccatg
      241 agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtattg aaaaatataa
      301 catatccaac tgattgtatt gaaaatttaa aatagccata taacaaaagg ttacacataa
      361 gctacttttt ggggtttcag gcaagaaact aaaaattatt aacgccatca aattattcac
      421 atcttaataa ttagcattga aatttaatgt ttttggttct ttgtacatgt caatggcttg
      481 tctttgtggc agaatcataa agctatgcaa tcattgcatt gttattaaca cagcatattt
      541 ttatatactt ttaacacctt acctcaaaaa ggataacaaa gtggacagaa gtgcggttga
      601 tacaattcgt gggtattgtt atcaggttga taaaacgatt attgagattt tttcgttacc
      661 acaaatggat gactcgattg atatagagtg cattgaagat gttgatgtct acaacgatgg
      721 gcatttaact gcgatacaat gcaaatatta tgaaagtacc gattataacc actccgttat
      781 atcaaagccc ataagattaa tgttgtcaca ctttaaggac aataaagaaa aaggggctaa
      841 ttattatctt tatgggcatt ataaatccgg tcaagaaaag ttaacactcc cattaaaagt
      901 tgactttttc aaatctaatt tcctcaccta caccgaaaaa aaaatcaaac atgaatacca
      961 tattgaaaat gggcttaccg aagaggatct acaagccttt ttggatcggt tagttataaa
     1021 tatcaatgca aaatcatttg atgatcaaaa aaaagaaact atacaaataa taaaaaacca
     1081 tttccaatgt gaagattatg aggcagagca ttatctttat tctaatgctt tcagaaaaac
     1141 atatgatatc tcttgtaata aaaaagatag aaggataaaa aaatctgatt ttgttgaaag
     1201 tatcaacaaa tcaaaagtct tatttaacat atggttttat caatatgaag gaagaaaaga
     1261 atatttaaga aaattaaaag aatctttcat acgcagaagt gtaaacacct caccttatgc
     1321 tcgttttttc atcttagaat ttcaagacaa aactgatata aaaacagtta aagactgtat
     1381 atataaaata caatcaaatt ggtctaattt atctaaaaga acagatcgac catattctcc
     1441 ttttttactt tttcatggca ccagcgatgc caatttatac gaattaaaga atcaattatt
     1501 caatgaagat ctaattttca ctgatgggta cccttttaaa ggaagtgtat ttacccccaa
     1561 gatgttaatc gaaggttttt caaataaaga aatccacttc caatttatca acgacataga
     1621 tgatttcaat gaaacactga acagtattaa tataagaaaa gaagtttacc agttttatac
     1681 ggaaaactgc cttgatatcc catcccaact accccaggta aacatacaag ttaaagactt
     1741 tgccgacata aaggagatag tgtaatgagc aggaataatg atattaatgc agaagtagta
     1801 tcggtatcgc caaataaatt aaaaatttcc gtagacgatc ttgaagaatt taagatagca
     1861 gaagaaaaat taggtgtagg atcttattta agggtttcag ataatcaaga tgttgctctt
     1921 ctggcgatca tagataattt ttctattgaa gttaaagaaa gccaaaagca gaaatacatg
     1981 atagaagcaa gtccaatagg tcttgttaaa aatggaaaat tctatcgcgg tggagattca
     2041 cttgcacttc ctcctaaaaa agtggaacca gcgaaattag acgaaataat atccatatac
     2101 tcagatagta tagatataaa tgaccgtttt actttttcaa gcttatcgct taataccaaa
     2161 gtatccgtac ctgtgaatgg gaatagattt ttcaataaac atatcgctat cgtaggttca
     2221 acgggttcag gtaaatccca cactgttgca aaaatacttc aaaaagccgt agatgaaaag
     2281 caagaaggtt ataagggatt aaacaattct catataatta tttttgatat acattctgaa
     2341 tatgaaaatg cattccctaa ttcaaatgta ttaaatgtag atacattaac ccttccatat
     2401 tggctattaa atggtgacga gttagaagag ctttttcttg acacggaagc aaatgatcac
     2461 aatcaaagaa atgtgttccg tcaggcaata acattaaata aaaagataca ttttcaagga
     2521 gatccagcca caaaggaaat aataagcttt cactcgccat attatttcga cattaatgaa
     2581 gtcatcaatt atattaacaa tagaaataat gaaagaaaaa ataaagataa tgaacatatt
     2641 tggtcagatg aggaaggaaa tttcaagttt gacaatgaaa atgctcatag gttattcaaa
     2701 gagaatgtaa ctcctgatgg aagttcagcc ggtgctttaa atggaaaact tctcaatttt
     2761 gttgatcgat tacaaagtaa aatatttgat aagagattag attttattct gggtgaaggt
     2821 agcaaatccg taacatttaa agaaacatta gaaactttaa taagctatgg aaaagataaa
     2881 tcaaacataa caatacttga tgtaagcggt gttccttttg aagtacttag catatgtgta
     2941 tcattgatat ctcgattaat ttttgaattt ggctatcatt caaaaaaaat aaaaagaaaa
     3001 tctaatgaaa accaagatat cccaatatta attgtttacg aagaagcaca taaatatgct
     3061 cccaaaagtg atctgagcaa atacaggaca tccaaagaag caattgagag gattgcaaaa
     3121 gagggtagaa aatacggagt aacccttctc cttgcaagtc agagaccttc tgaaatttca
     3181 gaaacaatat tttctcagtg taatactttt atctcaatgc gattaactaa cccagacgat
     3241 caaaattatg ttaagcgatt actcccggat acagtaggtg atattacaaa cctcctacca
     3301 tcgctcaaag aaggtgaggc cttaatcatg ggggattcaa tatcaatacc ttcgattgta
     3361 aaaatagaaa aatgtacaat acccccatcg tcaattgaca tcaaatatct tgatgaatgg
     3421 agaaaagaat gggtagattc ggagtttgat aagataattg aacaatggag taaaagttaa
     3481 tttcagaagt ggattcactc ttgctcaaga gtgaatccac taatatcata tcctaatgat
     3541 atagtttaat aaaatctatt ctggaatcat taggctgaga gcgcaataac tagcataacc
     3601 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
     3661 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     3721 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     3781 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat
     3841 aactgcctta aaaaaattac gccccgccct gccactcatc gcaatactgt tgtaattcat
     3901 ttaacattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
     3961 gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg ggggcgaaga
     4021 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     4081 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     4141 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     4201 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     4261 cccatatcac cagctcaccg tctttcatcg ccatacggaa ctctggatga gcattcatca
     4321 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     4381 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     4441 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     4501 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     4561 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     4621 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     4681 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     4741 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     4801 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     4861 agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
     4921 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
     4981 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     5041 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     5101 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     5161 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     5221 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     5281 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     5341 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     5401 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     5461 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     5521 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     5581 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     5641 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     5701 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aat
//
