LOCUS       sequence-318933-        6546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the dsr1 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG022 (dsr1)
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4404..4451
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4671..5330)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5245..5264
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5331..5433)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(5825..5842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5959..6504)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6039..6058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
       61 ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
      121 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
      181 gcatattttg cgtagctaga acgcaatcaa atctagcagt ccgctttgtt cggagttcgg
      241 acattatgag ttggcaagta aagtagcttg ctaggaagcc ggatttgcac ggtcggtata
      301 ataagatgta accccttgcc ttcatttact cgaatgaacg tgcacattgg ataggaggaa
      361 aaggaatgca attcattacc aacggccctg atattcctga tgagcttttg caggcgcacg
      421 aggaagggcg cgttgtgttc ttctgtggag caggcatttc ctaccctgct ggtttacctg
      481 gtttcaaagg gttggtagaa ctaatttacc agaggaacgg aacaacactt tcagaaattg
      541 agcgtgaggt tttcgagcgt gggcaatttg acggcacatt agatttgctg gaacggcgct
      601 taccagggca gcgtatagcc gtccgacgcg cgttggaaaa agcccttaag ccaaagctcc
      661 gtcgtagggg cgctattgat actcaggcgg cgctgttacg tttagcccgt agccgcgagg
      721 gtgcccttcg attggtcact accaactttg accgtctctt tcatgtggca gctaaacgta
      781 caggccaggc ttttcaggcc tatgtagcgc cgatgctgcc aattccaaaa aacagccgct
      841 gggatggact tgtatacctg catgggctgt taccggaaaa ggcggatgat actgccctga
      901 atcgtctggt tgttaccagc ggtgactttg gcttggctta tctcactgag cgttgggcag
      961 ctcgctttgt gagtgagtta tttcgtaact atgtggtctg cttcgttggc tacagcatca
     1021 acgacccggt actgcgctac atgatggatg cgcttgcagc agatcggagg ctcggtgaag
     1081 tcacaccaca agtatgggca ctgggggagt gtgagccggg gcaggagcac cggaaagcca
     1141 tcgagtggga ggccaaaggg gtcactccta tcctttacac cgtaccggcg ggctccactg
     1201 atcattcagt gctgcatcaa acgttgcacg cttgggcaga tacttatcga gatggtatac
     1261 agggcaaaaa ggctatagtc gtcaaacatg ctctggcccg cccgcaggac agcactcgtc
     1321 aggacgattt cgttggtcgg atgttgtggg ccttgtcaga taaatcaggt ttaccagcaa
     1381 aacgctttgc ggaactcaat cctgcaccgc cgctggattg gttattgaaa gctttctcgg
     1441 acgaacgatt taaatacagc gatctgccac gcttttgtgt atctccgcat gtcgaaattg
     1501 acccgaaact ccgattcagt ctggttcagc gtcctgcgcc ctatgagctg gccccgcaga
     1561 tgtcgctggt ttctggatgt gtcagtgcta gcaaatggga tgacgtaatg tcccatatag
     1621 cccgttggct agttcgttat ctgggcgacc ctaggttgat catatggatt gctgaacgcg
     1681 gcggacaaat acacgaccgt tggatgtttc tgattgagag cgaactagat cgcttagcag
     1741 cactgatgcg ggagcgtaag acttctgagt tagatgaaat tctcttgcat tcccccctgg
     1801 ctattcctgg tccacctatg tctactttat ggcggcttct gcttagtggt cgtgtgaaat
     1861 cgccattgca gaacctggat ttgtatcgtt ggcaaaaccg cttaaagaat gaaggcttga
     1921 cgactacatt gcgcttggag ttacgcgggt tgctttctcc caaggttatg ttgaggcggc
     1981 cgtttcgcta tagtgaagac gattcgagca gcactgatga acccttgcga atcaagcaat
     2041 tggtggattg ggagctggtg ctgactgctg attacgtacg ttcaaccctg ttcgaccttg
     2101 ctgacgagtc atggaaatcg tccttgccat acctgttgga agattttcag cagttgttgc
     2161 gtgatgcact ggacttgttg cgggagttgg gagagtccga cgatcgtcac gaccgctcgc
     2221 attgggattt gccgtccatc actccgcact ggcagaaccg ggggttccgc gattgggtga
     2281 gcctgattga attacttcgg gattcatggt tagccgttcg agccaaagac agcgatcagg
     2341 cctcgcgcat tgctcagaat tggtttgagt tgccatatcc caccttcaaa cgtctggcac
     2401 tgtttgccgc aagccaagac aactgcatac cacctgagcg gtgggttaat tggttgttag
     2461 aggacggttc atggtggttg tgggccacgg atactcggcg agaggtattc agactgtttg
     2521 ttttgcaggg acgacatctg acaggaattg cacaagagcg tctggaaact gctatcttgg
     2581 cagggcctcc gcgcgagatg tacgaggata atttggaagc agacaggtgg cattatttgg
     2641 tggctcattc cgtctggttg tgtctagcga agctcagggg agcgggcctt gttttgggag
     2701 agtctgcggc tacacgtttg acggaaatat ccacagcata cccaaaatgg caactggcaa
     2761 ccaacgagcg tgatgaattc tctcactgga tgagcggaac cggtgatcca ggcttcgagg
     2821 agagtataga tgtcgacatt gcgccccgta agtggcagga attagtgcaa tggctcgcaa
     2881 agcctatgcc agaaagactg cctttctatg aggacacttg gagtgatgtt tgccgtacgc
     2941 gcttttttca cagtctgtat gcgttacgta aactatcaca agatgatgtg tggcctgttg
     3001 gtcggtggcg tgaagctctg cagacttggg ctgaaccagg gatgattttg cgttcgtggc
     3061 ggtacgccgc accgttggtg cttgacatgc ctgacgcagt acttcaggag atttcccacg
     3121 ctgtcacttg gtggatggag gaggcttcga agaccatcct ctgccacgag gagattctac
     3181 tggccctttg tcgtcgggtt ctgatgatag aaacaagccc agagtctagc accattcgaa
     3241 acggaattga gacctatgat cctgtttcta cggcgatcaa tcatcccatt gggcatgtca
     3301 cgcaatcact gatcacccta tggttcaaac agaacccgaa tgacaatgat ttgcttcctg
     3361 ttgaattgaa aacacttttc accaaattgt gtaatgtaca gatagagcta ttccgccatg
     3421 gtcgggtgtt gctggggtcg cggctgatcg cattttttcg cgtagatcga ccttggaccg
     3481 aacagtatct attgcccttg tttgcttgga gtaatcccgt cgaagcaaaa gctgtgtggg
     3541 aaggcttcct ctggtcgcca cgcctgtatg aaccgttgct gatagctttc aagtcagatt
     3601 ttttggagag cgccaatcac tattctgatc ttggcgagca ccggcagcaa ttcgctattt
     3661 tcctgactta tgcagctctg ggccctaccg agggatatac cgtggaggag ttccgaacgg
     3721 caattagtgc tcttccacaa gaaggtctgg aggtagccgc gcaggcgtta taccaggcac
     3781 ttgaaggtgc gggcgatcag cgcgaggagt attggaaaaa tcgtgtccag ccattttggc
     3841 aacaggtttg gccaaagtcc cgcaacttgg ccaccccacg catatccgaa tcgttgactc
     3901 gtatggtgat tgctgcccga ggtgaatttc cggcggcttt ggcagtggtg caggactggc
     3961 tgcaaccgct cgaacacctt agctacgacg ttcgcctttt gctagaatca gatatttgca
     4021 gccgatatcc tgcggacgct ctatccctgc tgaatgccgt gattgccgaa caacactggg
     4081 ggcctcgaga gttggggcaa tgcttgcttc aaattgttca agctgctcca caactggagc
     4141 aagatgttcg ttatcagcga ttaaatgaat attctcgaag gcgcagcgtg tgaaagtgac
     4201 aggcgttgga cagtgcgaac tgtggagcct aacaaggtaa agacactcta actgataatg
     4261 ctgcgccgct cgtgcaatgc aatacagttt ttatctagcg gtgaattatg gtgttaaaag
     4321 ttagcccctg acacagggtg ggtagttggc tctgtgtcat tgatgggtat tagttctgat
     4381 atgagctaat acccacgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     4441 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
     4501 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     4561 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     4621 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     4681 ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca
     4741 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     4801 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     4861 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     4921 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     4981 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     5041 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     5101 atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     5161 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     5221 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     5281 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     5341 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     5401 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     5461 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     5521 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     5581 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     5641 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
     5701 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     5761 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     5821 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     5881 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     5941 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     6001 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6061 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     6121 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     6181 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     6241 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     6301 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     6361 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     6421 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     6481 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     6541 atttca
//
