LOCUS       sequence-318935-        7742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the qatABCD defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG027 (qatABCD)
FEATURES             Location/Qualifiers
     source          1..7742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1282..1293
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2942..2953
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      5487..5534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5754..6413)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6328..6347
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6414..6516)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6908..6925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7042..7587)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7122..7141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
       61 gcgttgcgca gaaattattt ggaatggatg atggcgcttg attactggaa caggtctatg
      121 acatgaaggt tatgatttgt tcactgctat gaggttaaca ctttaacaat ttcccttact
      181 attcttgtac taattccttc caaatacttc tgcttgagat taggatttat cctcttgtag
      241 tgttatttac aataaagatt gtgatgctga tttaacccaa cgtgttgtca gttgccttgc
      301 tgaactaagt tcagtatcta gaaattagct cttgatacat gagcgaatca gcgaaaattt
      361 tcatcccgac caattaatga ccgtaatgga taggatgttg ctgctatttg gcttccatga
      421 gggaacatat gtttttaaac gatcaagaaa cgtccactga cctgctgtac tacaccgcta
      481 tcgccagcac agtggttagg cttgttgatg aaacgtcaga tgcacccatt acgattggtg
      541 tgcatggtga ttggggggcg ggaaaatcaa gcgtactaaa aatgcttgag gctgcctgcg
      601 agaaaaagga taaaacgcac tgtatctggt ttaacggatg gacgtttgag ggattcgaag
      661 atgctaaaac tgtaatcatc gaaaccatcg tcgaggatct tgttgcctcg cgcccgatga
      721 gcaccaaggt ggcagaagca gcaaaaaagg ttcttcgtcg aattgactgg ttgaaaatgg
      781 ccaagaaagc ggggggactg gcgtttaccg catttactgg catacccaca tttgatcaga
      841 ttaaggggat gtacgaactg gcatccgact ttctaagtgc tccgcaggac aagctttctg
      901 ctgcagattt caaagcgttt gctgaaaaag caggaggctt catcaaagag gccgatactg
      961 atagtaatac gctacccaaa catattcatg ctttccgtga ggagttcagg gcgctgcttg
     1021 atgctgctga aattgaaaag ctagtggtga tcgttgacga tcttgatcgc tgcctgccta
     1081 aaaccgcgat tgaaacgctc gaagctattc gccttttctt gtttgtagag aaaactgcat
     1141 ttgttatcgg tgcagatgaa gccatgatcg aatatgcggt aaaagaccat ttccccgacc
     1201 tgcctcaaag caccgggccg gtaagttatg cacgcaacta tcttgaaaag ctcatacagg
     1261 ttccatttcg aatccccgca ctgggaactg cagaaacgcg tatatatacc acgttgttgc
     1321 ttgcagaaaa tgcgttgggt tcggaggacg acaattttaa agcattgctc aataaagcac
     1381 gggaagagat gaagcgtcct tggatcagcc gcgggcttga cagagaggca gtgatggcag
     1441 cgttaaatgg aaagattccg gaggttgtgg aaaacgcgct gctattcagc ctacacgtta
     1501 cccctatgct tagttcgggg acacatggta atccaaggca gattaaacgc tttttgaact
     1561 caatgatgtt acgccaggcg attgctgatg aacgcgggtt cggtagtgac attaagcgtc
     1621 ctgtactggc aaaaattatg cttgctgagc gtttttaccc cagcgtatac ggaaagcttg
     1681 ttcagcttgt atctaatcat ccagagggaa aaccggaagc tttggcggag tttgaagcct
     1741 tggtcagagg ggggaaaact gctccgaaga gtcgcgctga cagcaaagag aattcctcag
     1801 agtctgaaga cgtccaaaac tggctgaaga ttgattgggc gatcggttgg gcaaaagcag
     1861 agcccgcact ttctggagag gatcttcgtc catatgtgtt tgtcactcgt gacaaacaca
     1921 gtactttgag taatctggtc gtatcaagcc atctcattcc tataatggag aaacttcttg
     1981 gtccgaaaat tgggatggtg aaaatcaaag gggatttaga gaaactgagt ccaccggatg
     2041 ctgatgaatt attcgaaatg cttagcgata agcttttcca agaagacagt ttcaatcgaa
     2101 aaccaagagg atttgacggc ctcgaatatc tcgtagaaac acaacctcac cttcaaagga
     2161 gattgattga ttttgcacgg cgcattcctg taaaaaaagc agggggatgg cttgctaccc
     2221 gtattgcgca aagcctagtg gaccctacgt taatagaaga atatacaaaa ctgatccaag
     2281 aatgggcgag tcaggacgaa aatctgtccc tctctaaatc agcaaaagca accctccagt
     2341 tatcgggata tcaacattaa tgggaacctc aaaagcttac ggggggcctg ttcatggcct
     2401 aatccccgat ttcgtggaga atccatctcc accgaccctg ccgcctgttg accctgcgga
     2461 tgatagcacg ctggatacgc cgctcattcc accggattcg agtggctcag ggccacttag
     2521 cacaccgaaa gcaaacttta ctcgatactc ccgttcagga agtcgtagtt ctctgggtaa
     2581 ggcggtcgct ggatatgtcc gcaatggagt ggggggcgca ggcagggcca gccgccgtat
     2641 gggggcctca cgcgctgcag cagggggact gctcggtctc atcagcgact atcagcaggg
     2701 aggtgctact caggctcttg agcgcttcaa tcttggtaat ttggcagggc agtctgcatc
     2761 gactgctctt ctctcccttg ttgaattttt atgccctcca ggtggttctg ttgacgaggg
     2821 ggttgcgcgg caggctatgc tagagaccat cgccgatatg tctgatgtag gagaggagaa
     2881 ttttgatgag ctcactcccg atcaattaaa agaagtcttt attggtttcg tggttcactc
     2941 cattgaaggg aggctcatgg cggatattgg taaaaatggg atcaagttac cagacgacat
     3001 agacgctatc gtcagtatcc aggaggacct gcatgatttt gttgatggag ctactcgtac
     3061 acagctccgt gaggagctga ggaatcttac agggctttca ggggatgcta tagacagaaa
     3121 agtggaggag atttacaccg tggcatttga attacttgcc cgagaagggg agagattgga
     3181 atgagccatc ataccttagt tgcccgtttg ggcactgacg ataactccga tttacagctc
     3241 agccgccaaa gcacgcatct gacagaaatt aattttctca aagagaacgg taaactggat
     3301 ttcggtctcg ggcaggcgct gaatggtttg agtgatcttg gtttaacgcc aatggatgtc
     3361 tccgtggatc tggcactact ggccgcaacg gtgactgcgg cggacacccg aatctcacgt
     3421 gggcataacg ctcaagatct gtggacgcgc gaaattgcac tttatatccc ggtagcttcc
     3481 ccgacattat ggaatagtca gactggattg ctcagcagga tgttgaattt tcttaccggc
     3541 gaccgttgga caattcattt ccgctcgcgc cctgttattg agcacgggct cattcagcga
     3601 tcctctaagg aacgttcggt gaaccctact tctgtttgct tgttttccgg ggggctcgac
     3661 agcttcatcg gtgccattga tttattatct aatgggggaa ccccccttct gatcagccac
     3721 tactgggata cgactaccag cgtttatcag cagaagtgtg ctcagctgct gtcggagcga
     3781 tatggacaat cgttcagcca tgtgcgagct cgtgttgggt ttgaaaaaac aacgattgag
     3841 ggagaagatg gagaaaacac ccttcgtggc cgctctttca tgtttttctc gctcgcgaca
     3901 atggccgcag acgccctcgg cgggccggtc acgataaacg tccctgaaaa tggtttgatc
     3961 tctctcaacg ttcccctcga tccgcttcgt gtcggagcgc taagtactcg gacaacccat
     4021 ccgttttaca tggcgcgttt taatgagctg ctgggcaacc ttggcatcag tgcacatctg
     4081 gaaaatccct acgcctacaa aaccaaaggt gagatggcta tccattgcca tgaccatgct
     4141 tttctaaggc aacacgcggc tgacaccatg tcatgttcgt ctccgcaaag tacgcgttgg
     4201 aaccctgcgc tgaatgagca gcaatcaaca cactgtggcc gatgtgttcc atgcttaatc
     4261 aggcgagcat cattgtttac agctttcggc acggacgata cgatttaccg tatcccggat
     4321 ctccgtagcc gggtactgga cagctctaag cctgaaggtg aacacgttcg ggcatttcaa
     4381 tttgctctgg caagattggc gcgatcaccg agtcgagcaa aatttgatat tcacaaacca
     4441 gggccgctca gcgactatcc cgactgctta gctgagtatg aaggtgttta tctgagagga
     4501 atgaaagaag ttgaacgcct gctgagtgga gtcataacga ggccccttac atgaaattag
     4561 caggacagaa gcccgctcca caatgggtcg attttcactg tcatctggat ctatacccca
     4621 atcactctgc actcatccgt gaatgtgaca tttcacgtgt tgccacgcta gcggtgacga
     4681 caacccccaa ggcatggatg cgtaaccggg agttaacttc cgattctcct tatgttcgtg
     4741 tcgcacttgg tctacatccc cagctgattg cggaacgtga gcatgagata gcgttactgg
     4801 agcactatct cccttctgca cgttacgttg gggagatagg gcttgatgcc agcccgcgct
     4861 tttatcgcag ctttgaagca caggagcgga ttttttcccg tattctgaat gcctgtttcg
     4921 agcaggggga taagattctc agcatccaca gcgttcgcgc tgcagccaaa gtgttgggac
     4981 atttggaaaa caccagactt actgaaaatt gcaaggctgt cctacactgg ttcactggga
     5041 gtatctccga ggctcgacga gctgttgaac ttggatgcta tttctctatt aatgaagaga
     5101 tgctacgttc tcctaaacat cgaaagctgg tgtccttttt gcctttcgaa cgtatcttga
     5161 cggagaccga tggacctttt gtgtttcacg aagaaaaagc gatacaccct cgtgatgtgc
     5221 agcgtacggt tcatgaaatc gcgcagatcc accacgtatc ggacacagat gctgctatga
     5281 gaatacttta taatcttcga agtttagtca ccaatagttc tcacagtgag aatagttcat
     5341 gaatctaatt agttggatta atacagggga atagttgaat acttcagtcc cctaaaagct
     5401 aatatgctct atgtcatcta atgataagtg gctccaaaga gccacttatc attaactttt
     5461 ctaaagggag gtagaagtcg caataactag cataacccct tggggcctct aaacgggtct
     5521 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
     5581 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
     5641 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact
     5701 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
     5761 ccgccctgcc actcatcgca atactgttgt aattcattta acattctgcc gacatggaag
     5821 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5881 gtataatatt tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     5941 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     6001 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     6061 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     6121 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     6181 ttcatcgcca tacggaactc tggatgagca ttcatcaggc gggcaagaat gtgaataaag
     6241 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     6301 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     6361 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     6421 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     6481 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg
     6541 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct
     6601 tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt
     6661 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
     6721 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
     6781 gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat
     6841 gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt
     6901 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg
     6961 cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga
     7021 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     7081 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7141 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     7201 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     7261 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
     7321 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
     7381 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
     7441 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
     7501 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag
     7561 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc
     7621 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat
     7681 aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     7741 ag
//
