LOCUS       sequence-318943-        4282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rifampicin version of the scarless genome engineering pDEL plasmid 
            (Tikh et al. 2016) compatible with Salmonella.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, 
            Raleigh EA
  TITLE     Reassembling a cannon in the DNA defense arsenal: Genetics of StySA,
            a BREX phage exclusion system in Salmonella lab strains.
  JOURNAL   PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 
            10.1371/journal.pgen.1009943. eCollection 2022 Apr.
  PUBMED    35377874
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2022-04-4"; volume: "18(4):e1009943. doi"; pages: " 
            10.1371/journal.pgen.1009943. eCollection 2022 Apr"
COMMENT     Sequence Label: pOD_009
FEATURES             Location/Qualifiers
     source          1..4282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        738..856
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     protein_bind    2701..2722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2737..2742,2743..2760,2761..2767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2775..2791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2780..2802
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             join(2861..2947,2948..4282)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 cgtttaaggg caccaataac tgcagcgcta gtacggttat ccacagaatc aggggataac
       61 gcaggaaaga acatgtgaga tcagcagttc aacctgttga tagtacgtac taagctctca
      121 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
      181 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
      241 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
      301 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct agcttttaag
      361 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
      421 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatggcctt agaaggcgtt
      481 ttagaagccc taatggatgg agtcaaaaca aactagcgat ctgacgctca gtggaacgaa
      541 aactcacgtt aagggatttt ggtcattgta attctccatg gtaattcacg acggaggcca
      601 cctcatgtta gtcatgcgca aaaaaccccg cttcggcggg gttttttcgc gcccgccgaa
      661 aaccccgctt cggcggggtt ttgccgctca gctactaggg ataacagggt aatgaccagg
      721 tccttcagaa cgttcagcac cacaattcag caaattgtga acatcatcac gttcatcttt
      781 ccctggttgc caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct
      841 ttttagactg gtcgtagttt aactttaagg aggggatcta tgaagaacat caaaaagaat
      901 caagtaatca atttcggacc taattctaaa ttattaaaag aatataaatc acaattaatt
      961 gaattaaata ttgaacaatt tgaagcaggt attggtttaa ttttaggaga tgcttatatt
     1021 cgtagtcgtg atgaaggtaa actatattgt atgcaatttg agtggaaaaa taaggcatac
     1081 atggatcatg tatgtttatt atatgatcaa tgggtattat cacctcctca taaaaaagaa
     1141 agagttaatc atttaggtaa tttagtaatt acctggggag ctcaaacttt caaacatcaa
     1201 gcttttaata aattggctaa cttattcatt gtaaataaca aaaagcttat tcctaacaat
     1261 ttagttgaaa attacttgac acctataagt ttggcatact ggtttataga tgatggaggt
     1321 aaatgggatt acaataaaaa ctctcttaat aaaagtatcg tattaaatac acaaagtttt
     1381 actttcgaag aagtagaata tttggttaaa ggtttaagaa ataaattcca attgaattgt
     1441 tatgttaaaa tcaataaaaa caaaccaatc atctacattg attctataag ttatttaatc
     1501 ttttataatt tgatcaaacc ttacttaatc cctcaaatga tatacaaatt acctaatact
     1561 atctcatccg aaactttctt gaaataacat ggcgatagct agactgggcg gttttatgga
     1621 cagcaagcca ccaaacaccc cccaaaacca agccactgga gcacctcaaa aacaccatca
     1681 tacactaaat cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     1741 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     1801 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     1861 accataatga aataagatca ctaccgggcg tattttttga gttatcgagt tcaggagcta
     1921 aggagctaaa atggtaaaag attggattcc catctctcat gataattaca agcaggtgca
     1981 aggaccgttc tatcatggaa ccaaagccaa tttggcgatt ggtgacttgc taaccacagg
     2041 gttcatctct catttcgagg acggtcgtat tcttaagcac atctactttt cagccttgat
     2101 ggagccagca gtttggggag ctgaacttgc tatgtcactg tctggcctcg agggtcgcgg
     2161 ctacatatac atagttgagc caacaggacc gttcgaagac gatccgaatc ttacgaacaa
     2221 aaaatttccc ggtaatccaa cacagtccta tagaacctgc gaacccttga gaattgttgg
     2281 cgttgttgaa gactgggagg ggcatcctgt tgaattaata aggggaatgt tggattcgtt
     2341 agaggactta aagcgccgtg gtttacacgt cattgaagac tagttttttt aaggcagtta
     2401 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
     2461 cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc
     2521 gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc
     2581 ttctcaaatg cctgaggcca gtttgctcag gctctccccg tgtggtgtgg tgtggttgtg
     2641 tggggttcga aatgaccgac caagcgacat cataacggtt ctggcaaata ttctggcaat
     2701 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2761 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagcc agtccgttta
     2821 ggtgttttca cgagcaattg accaacagga ggatctggaa atgaacatca aaaagtttgc
     2881 aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg caactcaagc
     2941 gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt cccatattac
     3001 acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc aagttcctga
     3061 gttcgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg tttgggacag
     3121 ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc acatcgtctt
     3181 tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt tctatcaaaa
     3241 agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta aagacagcga
     3301 caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt caggttcagc
     3361 cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg gtaaacatta
     3421 cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca gctctttgaa
     3481 catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa cgtatcaaaa
     3541 tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata cgctgagaga
     3601 tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa acactggaac
     3661 tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg gcaaaagcac
     3721 atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac gcacggctga
     3781 gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac tgaaaaaagt
     3841 gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg cgaacgtctt
     3901 taaaatgaac ggcaaatggt atctgttcac tgactcccgc ggatcaaaaa tgacgattga
     3961 cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt taactggccc
     4021 atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc ctaacgatgt
     4081 aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg tcgtgattac
     4141 aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg cgcccagctt
     4201 cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc ttgaacaagg
     4261 acaattaaca gttaacaaat aa
//
