LOCUS       sequence-318952-        1425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express VN-dCasE-VC protein in mammalian cells. This plasmid is also
            referred to as as VN-dEcCas6-VC-GK in the publication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Gao F, Zheng K, Li YB, Jiang F, Han CY
  TITLE     A Cas6-based RNA tracking platform functioning in a 
            fluorescence-activation mode.
  JOURNAL   Nucleic Acids Res. 2022 May 6;50(8):e46. doi: 10.1093/nar/gkac014.
  PUBMED    35061906
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2022-05-6"; volume: "50(8):e46. doi"; pages: " 
            10.1093/nar/gkac014"
COMMENT     Sequence Label: VN-dCasE-VC-GK
FEATURES             Location/Qualifiers
     source          1..1425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..462)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIT"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      526..535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1171..1422
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1359..1380
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccctcgagag acctgcttgt
      481 aaaattccaa acgacctgaa gcagaaagtg atgaaccaca agcttgccac catggtgtat
      541 ctcagtaaag tcatcattgc cagggcctgg agcagggatc tttaccaact tgcccaggga
      601 ttatggcatt tatttccaaa cagaccggat gctgctcgtg attttctttt tcatgttgag
      661 aagcgaaaca caccagaagg ctgtcatgtt ttattgcagt cagcgcaaat gcctgtttca
      721 actgccgttg cgacagtcat taaaactaaa caggttgaat ttcaacttca ggttggtgtt
      781 ccactctatt ttcggcttcg ggcaaatccg atcaaaacta ttctcgacaa tcaaaagcgc
      841 ctggacagta aagggaatat taaacgctgt cgggttccgt taataaaaga agcagaacaa
      901 atcgcgtggt tgcaacgtaa attgggcaat gcggcgcgcg ttgaagatgt gcatcccata
      961 tcggaacggc cacagtattt ttctggtgat ggtaaaagtg gaaagatcca aacggtttgc
     1021 tttgaaggtg tgctcaccat caacgacgcg ccagcgttaa tagatcttgt acagcaaggt
     1081 attgggccag ctaaatcgat gggatgtggc ttgctatctt tggctccact gctgcagtcg
     1141 acgacctgca cagctggcgc tgaattcgcc gacaagcaga agaacggcat caaggccaac
     1201 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     1261 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1321 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1381 accgccgccg ggatcactct cggcatggac gagctgtaca agtaa
//
