LOCUS       sequence-318956-        2809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcribe ACTB-16×CBS mRNA as target for VN-dCasE-VC 
            (VN-dEcCas6-VC-GK) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Gao F, Zheng K, Li YB, Jiang F, Han CY
  TITLE     A Cas6-based RNA tracking platform functioning in a 
            fluorescence-activation mode.
  JOURNAL   Nucleic Acids Res. 2022 May 6;50(8):e46. doi: 10.1093/nar/gkac014.
  PUBMED    35061906
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2022-05-6"; volume: "50(8):e46. doi"; pages: " 
            10.1093/nar/gkac014"
COMMENT     Sequence Label: Actin-16×CBS-GK
FEATURES             Location/Qualifiers
     source          1..2809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2498..2550
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2688..2809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2725..2744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2779..2798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatggatga tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg
      601 gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc catcgtgggg cgccccaggc
      661 accagggcgt gatggtgggc atgggtcaga aggattccta tgtgggcgac gaggcccaga
      721 gcaagagagg catcctcacc ctgaagtacc ccatcgagca cggcatcgtc accaactggg
      781 acgacatgga gaaaatctgg caccacacct tctacaatga gctgcgtgtg gctcccgagg
      841 agcaccccgt gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc gagaagatga
      901 cccagatcat gtttgagacc ttcaacaccc cagccatgta cgttgctatc caggctgtgc
      961 tatccctgta cgcctctggc cgtaccactg gcatcgtgat ggactccggt gacggggtca
     1021 cccacactgt gcccatctac gaggggtatg ccctccccca tgccatcctg cgtctggacc
     1081 tggctggccg ggacctgact gactacctca tgaagatcct caccgagcgc ggctacagct
     1141 tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg
     1201 ccctggactt cgagcaagag atggccacgg ctgcttccag ctcctccctg gagaagagct
     1261 acgagctgcc tgacggccag gtcatcacca ttggcaatga gcggttccgc tgccctgagg
     1321 cactcttcca gccttccttc ctgggcatgg agtcctgtgg catccacgaa actaccttca
     1381 actccatcat gaagtgtgac gtggacatcc gcaaagacct gtacgccaac acagtgctgt
     1441 ctggcggcac caccatgtac cctggcattg ccgacaggat gcagaaggag atcactgccc
     1501 tggcacccag cacaatgaag atcaagatca ttgctcctcc tgagcgcaag tactccgtgt
     1561 ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc
     1621 aggagtatga cgagtccggc ccctccatcg tccaccgcaa atgcttctga gatctcgacc
     1681 gagttccccg cgccagcggg gataaaccgc gccagcttcg aattctgcag tcgaagagtt
     1741 ccccgcgcca gcggggataa acccgatcca ccggtattta tctcgaccga gttccccgcg
     1801 ccagcgggga taaaccgcgc cagcttcgaa ttcgagttcc ccgcgccagc ggggataaac
     1861 ccgatccacc ggtatttatc tcgaccgagt tccccgcgcc agcggggata aaccgcgcca
     1921 gcttcgaatt ctgcagtcga agagttcccc gcgccagcgg ggataaaccc gatccaccgg
     1981 tatttatctc gaccgagttc cccgcgccag cggggataaa ccgcgccagc ttcgaattcg
     2041 agttccccgc gccagcgggg ataaacccga tccaccggta tttatctcga ccgagttccc
     2101 cgcgccagcg gggataaacc gcgccagctt cgaattctgc agtcgaagag ttccccgcgc
     2161 cagcggggat aaacccgatc caccggtatt tatctcgacc gagttccccg cgccagcggg
     2221 gataaaccgc gccagcttcg aattcgagtt ccccgcgcca gcggggataa acccgatcca
     2281 ccggtattta tctcgaccga gttccccgcg ccagcgggga taaaccgcgc cagcttcgaa
     2341 ttctgcagtc gaagagttcc ccgcgccagc ggggataaac ccgatccacc ggtatttatc
     2401 tcgaccgagt tccccgcgcc agcggggata aaccgcgcca gcttcgaatt cgagttcccc
     2461 gcgccagcgg ggataaaccc gatccaccgg tatttatctc gagctcaagc ttcgaattct
     2521 gcagtcgacg gtaccgcggg cccgggatcc atatgtaagt aagtaagcgg ccgcgactct
     2581 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2641 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatctta
//
