LOCUS       sequence-318957-       10252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1184..1208
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1283..1292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1289..1291,1292..1294,1295..2005)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(1334..1355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2033..2089
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2102..2500
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2551..2775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3634..4443)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4357..4376
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4435..4454
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(4602..5190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4682..4701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6059..6096
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             6107..6706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6107..6126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6603..6623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6819..6838)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6884..6939
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6938..6957)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      8224..8233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8230..8928
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    8979..9203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(10252,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gagagctcgt ttagtgaacc
     1201 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga gaacgggacc
     1261 gatccaaatt ctcgaggcta gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     1321 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag
     1381 gggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
     1441 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg
     1501 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1561 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc
     1621 cgaggtgaag ttcgagggcg acaccctagt gaaccgcatc gagctgaagg gcgtcgactt
     1681 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacat
     1741 ctatatcatg gccgtcaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1801 cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1861 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga
     1921 ccccaacgag aagcgcgatc acatggtcct gctggagttc cgcaccgccg ccgggatcac
     1981 tctcggcatg gacgagctgt acaaggaatt cggtagcaag cttggtagtg gagcaactaa
     2041 cttttccctg ctcaaacaag ccggggatgt agaggagaac cccggccccg ccccccttaa
     2101 gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2161 caacagcatc cccatctctg aggactacag cgtcgccagc gcagctctct ctagcgacgg
     2221 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2281 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2341 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2401 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2461 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgctc gagtctagag
     2521 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     2581 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     2641 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     2701 gggtggggca ggacagcaag ggggaggatt gggaagtcaa tagcaggcat gctggggatg
     2761 cggtgggctc tatggcttct gaggcaggtc gaagaactat ttcctggctg ttacgcgagc
     2821 gcatcgcgat aagctctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
     2881 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     2941 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     3001 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     3061 acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct
     3121 gcgtacggat ggggctctag ctctagagct tgatatcgaa ttcctgcagc cccgctagag
     3181 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
     3241 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
     3301 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
     3361 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct
     3421 gaaagagaga tttagaatga cagaatcata gaacggcctg ggcgcgcctg gtggcgaacg
     3481 atcaacagac gggagaggac tagaggatgc acatgtgacc gagggatgga acgacgcgta
     3541 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct
     3601 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     3661 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     3721 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     3781 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     3841 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     3901 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     3961 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     4021 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     4081 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     4141 tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     4201 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     4261 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     4321 caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     4381 taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct
     4441 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     4501 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccatg ctagctccag
     4561 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4621 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4681 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4741 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4801 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4861 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4921 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4981 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5041 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5101 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5161 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5221 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5281 atcttcacct agatcctttc tcgagctttc ggagtgagat ggagtggcca ctgtggaact
     5341 gggcctagca tcgcgataag ctctagaggg acagcccccc cccaaagccc ccagggatgt
     5401 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
     5461 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
     5521 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
     5581 gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga
     5641 acggcctgcg tacggatggg gctctagctc tagagcttga tatcgaattc ctgcagcccc
     5701 gctagaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     5761 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     5821 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     5881 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     5941 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgggc gcgcctggtg
     6001 gcgaacgatc aacagacggg agatctacta ttgggacagc agagatccag ttggtaatgg
     6061 cttgtcgacg acggcggtct ccgtcgtcag gatcataggt gccaccatga ccgagtacaa
     6121 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     6181 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     6241 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6301 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6361 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6421 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6481 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6541 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc
     6601 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6661 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagctt ccactggatt
     6721 gtacaattac caactgaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     6781 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     6841 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     6901 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     6961 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     7021 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     7081 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     7141 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     7201 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     7261 agatccgggc ctagcatcgc gataagctct agagggacag ccccccccca aagcccccag
     7321 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     7381 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     7441 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     7501 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat
     7561 catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc gaattcctgc
     7621 agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     7681 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     7741 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     7801 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     7861 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggcgcgc
     7921 ctggtggcga acgatcaaca gacgggagat tgccaaggtc ttgacaaacg agtgctagta
     7981 caagatcgga gtgaggaaaa ttagagtgag gacgatcgga gtgaggataa ttagagtgag
     8041 gacgatcgga gtgaggaaaa ttagagtgag gacgatcgga gtgaggataa ttagagtgag
     8101 gacgatcgga gtgaggaaaa ttagagtgag gacgatcgga gtgaggataa ttagagtgag
     8161 gacgatccct ctagagggta tataatgggg gccactagtc tactaccaga gctcatcgct
     8221 agcgccacca tggtgagcga gctgattaag gagaacatgc acatgaagct gtatatggag
     8281 ggcaccgtga acaaccacca cttcaagtgt acctccgagg gcgaaggcaa gccctacgag
     8341 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac
     8401 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
     8461 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac
     8521 gaagatgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     8581 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     8641 acactcggct gggaggcctc caccgaaacc ctgtaccccg ctgacggcgg cctggaaggc
     8701 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaaaacc
     8761 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     8821 aggagactgg aaagaatcaa ggaggccgac aaagaaacct acgtcgagca gcacgaggtg
     8881 gctgtggcca gatactgcga cctccctagc aaactggggc acagatgagc ggccgctcga
     8941 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9001 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9061 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     9121 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc
     9181 tggggatgcg gtgggctcta tggcttctga ggcaggtcga gggtacatgc gccttactcc
     9241 ttgtgagcgc atcgcgataa gctctagagg gacagccccc ccccaaagcc cccagggatg
     9301 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     9361 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     9421 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     9481 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     9541 aacggcctgc gtacggatgg ggctctagct ctagagcttg atatcgaatt cctgcagccc
     9601 cgctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     9661 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     9721 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     9781 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     9841 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg cgcgcctggt
     9901 ggcgaacgat caacagacgg gagagggcaa aaccgatgtt gacggactaa tcctgacaag
     9961 atctggggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    10021 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    10081 acgggccaga tatacgcgtg agtaattcat acaaaaggac tcgcccctgc cttggggaat
    10141 cccagggacc gtcgttaaac tcccactaac gtagaaccca gagatcgctg cgttcccgcc
    10201 ccctcacccg cccgctctcg tcatcactga ggtggagaag agcatgcgtg ag
//
