LOCUS       sequence-31900-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Wu W, Lin F, Worman HJ
  TITLE     Intracellular trafficking of MAN1, an integral protein of the 
            nuclear envelope inner membrane.
  JOURNAL   J Cell Sci. 2002 Apr 1. 115(Pt 7):1361-71.
  PUBMED    11896184
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2002 Apr 1. 115(Pt 7):1361-71."
COMMENT     Sequence Label: pSVK3-Flag-MAN1
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctatcga attaatacga
       61 ctcattatag ggagatcgaa ttcgagctcg gtacccgggg atcctctagt ggatccgcca
      121 ccatggacta caaggacgac gatgacaaag atcccccggg ctgcaggaat tcggacgaga
      181 aaatggcggc ggcagcagct tcggcgcctc agcagctctc ggatgaggag cttttctctc
      241 agctccgccg ttacggcctg tctcccggac cagtgacgga gagcacccgc ccggtctacc
      301 tcaagaagct gaagaagctt cgagaggaag agcagcaaca gcaccggtca gggggccgcg
      361 gcaacaagac gcggaacagt aataacaata acacggcagc cgccacggtc gcagccgcgg
      421 gaccagcggc ggcggcggcc gcggggatgg gggtccggcc ggtctcgggc gacctctcct
      481 acttacggac tcctgggggc ctgtgccgaa tctcggcctc tggcccagag agcctcctgg
      541 gagggcccgg gggcgcctcc gccgcccccg cggctggcag caaagtgctg ctgggcttca
      601 gctcggacga gtcggacgtg gaggccagtc cccgggacca ggccggcggc ggcgggagga
      661 aagaccgggc ttcgctccag taccgcgggc tcaaagcgcc gccggcgccc ctggccgcca
      721 gcgaggtgac taacagcaac tctgcagagc gaaggaagcc ccactcgtgg tggggg
//
