LOCUS       sequence-31908-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: p21 shRNA1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(539..583)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aaaaatattc aagagatatt acggttgagt cctaattttt tgtctagaga tctaagggcg
       61 aattctttcg gatcctttct taattaacca aactggatct ctgctgtccc tgtaataaac
      121 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
      181 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
      241 attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca
      301 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
      361 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc
      421 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta
      481 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca
      541 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg
      601 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca
      661 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag
      721 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat
      781 cctttaggta tctttccaca gccaggattc
//
