LOCUS       sequence-31909-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: p21 shRNA2
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    120..237
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(535..579)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggattcaaga gatccttgta caactgctac tttttttgtc tagagatcta agggcgaatt
       61 ctttcggatc ctttcttaat taaccaaact ggatctctgc tgtccctgta ataaacccga
      121 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
      181 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg
      241 tggatgaata ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa
      301 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
      361 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
      421 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
      481 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
      541 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
      601 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
      661 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
      721 agcagtggtg caaatgagtt ttccagagca acccc
//
