LOCUS       sequence-319096-        6573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6573)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-ARP3-C-12
FEATURES             Location/Qualifiers
     source          1..6573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        121..424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        425..628
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     579..599
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             673..1350
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1399..2073
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    3493..3614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3530..3549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3584..3603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3621..4076)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3758..3779)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3970..3989
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4103..4207
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4232..4252)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4276..4486
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4298..4494
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4345..4480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4529..5323
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4529..5323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4583..4602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5193..5212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5511..5530)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5555..5602
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5931..6519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6420..6439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
       61 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      121 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      181 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      241 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      301 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      361 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      421 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      481 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      541 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      601 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      661 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
      721 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      781 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      841 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
      901 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      961 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
     1021 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
     1081 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1141 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1201 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1261 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1321 cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact
     1381 ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa
     1441 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     1501 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     1561 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata
     1621 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     1681 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     1741 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag
     1801 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt
     1861 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     1921 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     1981 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     2041 gctcattctg gattgcctga caatgccaga cgatccggac tcagatctgg tggaagcggc
     2101 gctggagggg cgggacggct gccggcctgt gtggtggact gtggcacggg gtatacaaaa
     2161 ctaggatatg ctggaaatac agaaccacag tttatcatcc cttcctgtat tgctattaag
     2221 gagtcagcaa aagtgggtga tcaagctcaa aggagggtga tgaaaggtgt tgatgaccta
     2281 gacttcttca ttggtgatga agcaatagaa aaacctacat atgcaacaaa gtggccaatc
     2341 cgccatggta tagttgaaga ttgggactta atggaaaggt ttatggagca agtgatcttt
     2401 aaatatttaa gggcagaacc tgaagaccat tattttcttt tgactgaacc tccattgaat
     2461 actccagaaa acagggaata tactgctgaa ataatgtttg agtccttcaa tgttccaggc
     2521 ttgtacattg ctgtgcaggc tgttcttgcc ttagctgcat cttggacctc aagacaagta
     2581 ggagaacgga cgttgaccgg tacggtaata gacagtggag atggtgtcac tcatgtcatt
     2641 cctgtggctg aagggtatgt gattggcagc tgtattaaac acattccaat cgcaggacga
     2701 gatataacat attttattca gcaactgctg agagaccgag aagtaggaat ccctccagaa
     2761 caatccttgg aaactgctaa ggcagtaaag gagcgctata gttatgtctg cccagattta
     2821 gtaaaagaat ttaacaagta tgatacagat gggtcaaaat ggattaaaca gtatactgga
     2881 atcaatgcta tctcaaagaa agagttttct atcgatgttg gttatgagag atttttggga
     2941 cctgaaatct tttttcatcc agagtttgct aatccagact ttacacaacc tatctcagaa
     3001 gttgtagatg aagtaattca gaattgtcct attgatgtca gacgtcctct ctacaagaat
     3061 attgtcctct ctggaggttc aaccatgttc agggactttg gacgtcgctt gcaaagagat
     3121 ttgaaaagaa ctgtagatgc ccggctgaaa ttaagtgagg aattgagtgg tggtagattg
     3181 aagccaaaac ctattgatgt acaagtcatt acacaccaca tgcagcgata tgcagtttgg
     3241 tttggaggat caatgctggc ttccacgcct gagttctacc aagtatgcca caccaaaaag
     3301 gattatgaag aaattggacc tagcatttgt cgtcacaatc cagtgtttgg agtcatgtcg
     3361 taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     3421 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3481 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3541 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3601 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3661 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3721 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3781 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3841 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3901 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3961 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4021 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     4081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4201 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     4501 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4561 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4621 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4681 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     4741 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4801 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4861 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4921 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4981 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5041 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5101 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5161 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5221 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5281 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5341 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5401 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5461 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     5521 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     5581 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     5641 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     5701 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     5761 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     5821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6541 ttacggttcc tggccttttg ctggcctttt gct
//
