LOCUS       sequence-31910-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: pFUGW-H1 empty vector
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    147..264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(562..606)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaggcttg gattgggatc ttataagttc tgtatgagac cactctttcc cgggaagaaa
       61 gctcctctag agatctaagg gcgaattctt tcggatcctt tcttaattaa ccaaactgga
      121 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
      181 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
      241 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagaattgg
      301 cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
      361 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
      421 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
      481 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
      541 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
      601 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
      661 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
      721 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
      781 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
      841 gagctgcttg atgccccaga ctgtgagttg cancagatgc tg
//
