LOCUS       sequence-319104-       16189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter without a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 16189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL13Rb
FEATURES             Location/Qualifiers
     source          1..16189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     783..805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     797..814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     798..814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          3814..4002
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      5431..5678
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             7030..7725
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     7431..7450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8330..8346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8343..8365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8354..8370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8378..8384,8385..8402,8403..8408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8423..8444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8635..9311
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9248..9268
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9374..10171
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9431..9450)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10041..10060
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10228..10402
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10480..10504
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10929..11723
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11810..12398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12299..12318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12552..12569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12584..12724
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12650..12669)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12810..12832
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13068..13262
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13328..14401)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14830..15459)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      841 ctctagagga tccgatatcg aattcctgca gcccggggga tcctacggta cttattgcca
      901 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
      961 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1021 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1081 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1141 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1201 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1261 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1321 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1381 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1441 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1501 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1561 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1621 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1681 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1741 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1801 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1861 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1921 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1981 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2041 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2101 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2161 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2221 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2281 atatggcctc cgtcgctcca gtattccagg gttcatcaga gtgccagagt ccttcgatag
     2341 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     2401 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     2461 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     2521 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     2581 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     2641 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     2701 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     2761 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     2821 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     2881 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     2941 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     3001 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     3061 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     3121 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     3181 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     3241 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     3301 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     3361 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     3421 cgccgtgtat ggtccgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc
     3481 tgtgggcatt cagtctggat cgcgaaaact gtggaattgg tcagcgttgg tgggaaagcg
     3541 cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg
     3601 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag
     3661 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
     3721 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     3781 tcacgccgta tgttattgcc gggaaaagtc tacgtaagtt tctgcttcta cctttgatat
     3841 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat
     3901 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt
     3961 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtatcacc gtttgtgtga
     4021 acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac gaaaacggca
     4081 agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat cgcagcgtaa
     4141 tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgtcgcgc
     4201 aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat gtcagcgttg
     4261 aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc gggactttgc
     4321 aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca
     4381 cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg
     4441 cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc
     4501 gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc
     4561 acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg
     4621 aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgctgctg
     4681 tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt
     4741 acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg attaaagagc
     4801 tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg
     4861 atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac
     4921 tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata
     4981 ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa
     5041 gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga
     5101 aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact
     5161 caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc
     5221 gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg
     5281 cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc
     5341 gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg
     5401 aaaaaccgca gcagggaggc aaacaatgaa tatgaagatg aagatgaata tttggtgtgt
     5461 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     5521 ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt
     5581 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     5641 tgctatggta tggactatgg aatatgatta aagataagat gggctcatag agtaaaacga
     5701 ggcgagggac ctataaacct cacttcatca tgctatttca tgatctattt tataaaataa
     5761 agatgtagaa aaaagtaagc gtaataaccg caaaacaaat gatttaaaac atggcacata
     5821 atgaggagat taagttcggt ttacgtttat tttagtacta attgtaacgt gagactacgt
     5881 atcgggaatc gcctaccggg taccggctgg atcttatgac gaccgttaga aattgtggtt
     5941 gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
     6001 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
     6061 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
     6121 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
     6181 caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
     6241 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
     6301 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
     6361 tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct
     6421 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtctaataa
     6481 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
     6541 aatttgtcga ttaatctgag tttttctgat taacagatgg cgaaggcaga taagaaacca
     6601 gcggagaaga aaccggcaga gaaaactccg gcagtcgaac cagcagcagc ggcagagaag
     6661 aaaccaaaag ccggaaagaa actccccaag gaaccagccg tcgccggaga caagaagaag
     6721 aagagatcaa agaagaacgt tgagacatac aagatctaca tcttcaaggt gttgaagcaa
     6781 gttcatccag acatcggaat ctcaagcaaa gccatgggaa tcatgaacag tttcatcaac
     6841 gatatctttg agaagcttgc tggtgagtct tcgaagcttg cgaggtacaa caagaagccg
     6901 acgattactt ccagggagat tcagactgcg gttagacttg tgttgcccgg tgagttggcg
     6961 aaacatgctg tttctgaagg gactaaggcg gttacgaagt ttacgagttc tgctggagct
     7021 ggagaattca tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
     7081 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     7141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     7201 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac caagcacccc
     7261 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     7321 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     7381 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     7441 cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     7501 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac
     7561 ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac
     7621 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     7681 tccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaggc tttttgtgat
     7741 ctgatgataa gtggttggtt cgtgtctcat gcacttggga ggtgatctat ttcacctggt
     7801 gtagtttgtg tttccgtcag ttggaaaaac ttatccctat cgatttcgtt ttcattttct
     7861 gcttttcttt tatgtacctt cgtttgggct tgtaacgggc ctttgtattt caactctcaa
     7921 taataatcca agtgcatgtt aaacaatttg tcatctgttt cggctttgat atactactgg
     7981 tgaagatggg ccgtactact gcatcacaac gaaaaataat aataagatga aaaacttgaa
     8041 gtggaaaaaa aaaaaaactt gaatgttcac tactactcat tgaccataat gtttaacata
     8101 catagctcaa tagtattttt gtgaatatgg caacacaaac agtccaaaac aattgtctct
     8161 tactatacca aaccaagggc gccgcttgtt tgccactctt tgtgtgcaat agtgtgatta
     8221 ccacatctcc acattcaata tattccctga attatctgac gattttgatg gctcactgtt
     8281 ttcccaagtc tggtacccca cctacatggc agctcgaatt cgtaatcatg tcatagctgt
     8341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8521 cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac
     8581 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     8641 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     8701 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     8761 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     8821 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     8881 atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     8941 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     9001 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     9061 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     9121 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     9181 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     9241 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     9301 ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat
     9361 ctgtcgatcg accatgggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     9421 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     9481 cgtgtttcgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     9541 tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     9601 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     9661 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     9721 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     9781 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     9841 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     9901 ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa
     9961 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    10021 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    10081 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    10141 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg atcgatcctc
    10201 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
    10261 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    10321 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
    10381 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
    10441 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    10501 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    10561 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
    10621 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
    10681 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
    10741 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
    10801 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
    10861 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
    10921 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    10981 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    11041 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    11101 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    11161 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    11221 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    11281 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    11341 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    11401 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    11461 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    11521 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    11581 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    11641 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    11701 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
    11761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11941 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12001 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12061 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    12121 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    12181 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12241 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12301 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12361 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12421 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12481 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12541 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    12601 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    12661 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
    12721 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    12781 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    12841 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
    12901 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
    12961 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
    13021 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
    13081 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
    13141 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
    13201 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
    13261 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
    13321 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
    13381 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
    13441 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
    13501 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
    13561 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
    13621 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
    13681 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
    13741 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
    13801 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    13861 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    13921 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    13981 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    14041 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    14101 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    14161 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    14221 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    14281 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    14341 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    14401 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
    14461 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    14521 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    14581 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    14641 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    14701 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    14761 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    14821 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    14881 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    14941 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    15001 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    15061 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    15121 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    15181 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    15241 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    15301 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    15361 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    15421 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    15481 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    15541 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    15601 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    15661 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    15721 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    15781 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    15841 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    15901 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    15961 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    16021 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    16081 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    16141 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcg
//
