LOCUS       sequence-319106-       11380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitous expression of PCP-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UPG
FEATURES             Location/Qualifiers
     source          1..11380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1760..2470
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1787..1815)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2410..2430
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3118..3134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3118..3134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3131..3153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3142..3158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3166..3172,3173..3190,3191..3196))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3211..3232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3423..4099
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4036..4056
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4166..5191
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5232..5406
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5484..5508
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5933..6727
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6814..7402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7303..7322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7556..7573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7588..7728
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7654..7673)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7814..7836
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8072..8266
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8332..9405)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9834..10463)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccagga tgatttctgg tttaaacaag cttcggctgg atcttatgac gaccgttaga
      721 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
      781 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
      841 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
      901 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
      961 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     1021 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     1081 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     1141 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     1201 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     1261 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     1321 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagatgc tagcctccaa
     1381 aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg actgagatcc agtccaccgc
     1441 agaccgtcag atcttcgaag agaaggtcgg gcctctggtg ggtcggctgc gcctcacggc
     1501 ttcgctccgt caaaacggag ccaagaccgc gtatcgcgtc aacctaaaac tggatcaggc
     1561 ggacgtcgtt gattccggac ttccgaaagt gcgctacact caggtatggt cgcacgacgt
     1621 gacaatcgtt gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc
     1681 cctcgtcgcg acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtgg
     1741 atccatggta gatctgacta gtaaaggaga agaacttttc actggagttg tcccaattct
     1801 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1861 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1921 tccgtggcca acacttgtca ctactttctc ttatggtgtt caatgctttt caagataccc
     1981 agatcatatg aagcggcacg acttcttcaa gagcgccatg cctgagggat acgtgcagga
     2041 gaggaccatc ttcttcaagg acgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     2101 gggagacacc ctcgtcaaca ggatcgagct taagggaatc gatttcaagg aggacggaaa
     2161 catcctcggc cacaagttgg aatacaacta caactcccac aacgtataca tcatggccga
     2221 caagcaaaag aacggcatca aagccaactt caagacccgc cacaacatcg aagacggcgg
     2281 cgtgcaactc gctgatcatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     2341 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2401 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2461 actatacaaa gctagcccat ggtaagcttt ttgtgatctg atgataagtg gttggttcgt
     2521 gtctcatgca cttgggaggt gatctatttc acctggtgta gtttgtgttt ccgtcagttg
     2581 gaaaaactta tccctatcga tttcgttttc attttctgct tttcttttat gtaccttcgt
     2641 ttgggcttgt aacgggcctt tgtatttcaa ctctcaataa taatccaagt gcatgttaaa
     2701 caatttgtca tctgtttcgg ctttgatata ctactggtga agatgggccg tactactgca
     2761 tcacaacgaa aaataataat aagatgaaaa acttgaagtg gaaaaaaaaa aaacttgaat
     2821 gttcactact actcattgac cataatgttt aacatacata gctcaatagt atttttgtga
     2881 atatggcaac acaaacagtc caaaacaatt gtctcttact ataccaaacc aagggcgccg
     2941 cttgtttgcc actctttgtg tgcaatagtg tgattaccac atctccacat tcaatatatt
     3001 ccctgaatta tctgacgatt ttgatggctc actgttttcc caagtctggt accccaccta
     3061 catggggatc ctaactcgag ctgcaggctt cggtaccgag ctcgaattcg taatcatgtc
     3121 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3181 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3241 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3301 ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag
     3361 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     3421 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     3481 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     3541 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     3601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     3661 gtggattgat gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
     3721 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
     3781 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
     3841 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
     3901 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     3961 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     4021 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     4081 tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc tctcgagctt
     4141 tcgcagatcc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     4201 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     4261 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     4321 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     4381 cccgattccg gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc
     4441 ccgccgttca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     4501 acaaccggtc gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     4561 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     4621 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     4681 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     4741 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     4801 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     4861 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     4921 gcatccggag cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga
     4981 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     5041 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     5101 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     5161 ccccagcact cgtccgaggg caaagaaata gagtagatgc cgaccgggat ctgtcgatcg
     5221 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
     5281 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     5341 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     5401 cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta
     5461 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
     5521 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
     5581 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
     5641 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
     5701 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa
     5761 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
     5821 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
     5881 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
     5941 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
     6001 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
     6061 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
     6121 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
     6181 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
     6241 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
     6301 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
     6361 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
     6421 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
     6481 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
     6541 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
     6601 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
     6661 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     6721 gttttagtac ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6961 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7201 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7441 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7501 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7561 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7621 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7681 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     7741 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7801 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7861 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     7921 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     7981 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     8041 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     8101 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     8161 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     8221 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     8281 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     8341 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     8401 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     8461 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8521 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     8581 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     8641 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     8701 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     8761 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     8821 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     8881 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     8941 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     9001 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     9061 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     9121 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     9181 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     9241 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     9301 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     9361 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     9421 ttgggggttc cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa
     9481 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9541 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     9601 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     9661 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     9721 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     9781 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     9841 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     9901 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     9961 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    10021 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    10081 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    10141 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    10201 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    10261 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    10321 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10381 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10441 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10501 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10561 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    10621 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    10681 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    10741 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    10801 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    10861 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    10921 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    10981 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    11041 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    11101 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    11161 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    11221 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    11281 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    11341 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
//
