LOCUS       sequence-31911-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S
  TITLE     shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway 
            in NSC self-renewal during development.
  JOURNAL   Cell Stem Cell. 2007 Jun 7. 1(1):87-99.
  PUBMED    18371338
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2007 Jun 7. 1(1):87-99."
COMMENT     Sequence Label: pFUGW-H1 empty vector
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(120..139)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ctaccatggt ggcgaccggt actagtctaa cactgaaaat tacatattga cccaaatgat
       61 tacatttcaa aaggtgccta aaaaacttca caaaacacac tcgccaaccc cgagcgcata
      121 gttcaaaacc ggagcttcag ctacttaaga agataggtac ataaaaccga ccaaagaaac
      181 tgacgcctca cttatccctc ccctcaccag aggtccggcg cctgtcgatt caggagagcc
      241 taccctaggc ccgaaccctg cgtcctgcga cggagaaaag cctaccgcac acctaccggc
      301 aggtggcccc accctgcatt ataagccaac agaacgggtg acgtcacgac acgacgaggg
      361 cgcgcgctcc caaaggtacg ggtgcactgc ccaacggcac cgccataact gccgcccccg
      421 caacagacga caaaccgagt tctccagtca gtgacaaact tcacgtcagg gtccccagat
      481 ggtgccccag cccatctcac ccgaataaga gctttcccgc attagcgaag gcctcaagac
      541 cttgggttct tgccgcccac catgcccccc accttgtttc aacgacctca cagcccgcct
      601 cacaagcgtc ttccattcaa gactcgggaa cagccgccat tttgctgcgc tccccccaac
      661 ccccagttca gggcaacctt gctcgcggac ccagactaca gcccttggcg gtctctccac
      721 acgcttccgt cccaccgagc ggcccggcgg ccacgaaang ccccggccag cccagcagcc
      781 cgctactcac caagtgacga tcacagcgat ccacaaacaa gaaccgcgac ccaaatcccg
      841 gctgcgacgg aactagctgt gccacacccg
//
