LOCUS       sequence-319112-       17603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the Arabidopsis HSP101 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 17603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL12R-HSP101
FEATURES             Location/Qualifiers
     source          1..17603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1190..1885)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1465..1484)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     RBS             4117..4128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     intron          7633..7821
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      9250..9497
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(9744..9760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9744..9760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9757..9779)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9768..9784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9792..9798,9799..9816,9817..9822))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9837..9858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10049..10725
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10662..10682
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10788..11585
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10845..10864)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11455..11474
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11642..11816
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11894..11918
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12343..13137
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13224..13812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13713..13732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13966..13983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13998..14138
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14064..14083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14224..14246
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14482..14676
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14742..15815)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16244..16873)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgataccatg taggtggggt accagacttg ggaaaacagt gagccatcaa aatcgtcaga
      661 taattcaggg aatatattga atgtggagat gtggtaatca cactattgca cacaaagagt
      721 ggcaaacaag cggcgccctt ggtttggtat agtaagagac aattgttttg gactgtttgt
      781 gttgccatat tcacaaaaat actattgagc tatgtatgtt aaacattatg gtcaatgagt
      841 agtagtgaac attcaagttt tttttttttt ccacttcaag tttttcatct tattattatt
      901 tttcgttgtg atgcagtagt acggcccatc ttcaccagta gtatatcaaa gccgaaacag
      961 atgacaaatt gtttaacatg cacttggatt attattgaga gttgaaatac aaaggcccgt
     1021 tacaagccca aacgaaggta cataaaagaa aagcagaaaa tgaaaacgaa atcgataggg
     1081 ataagttttt ccaactgacg gaaacacaaa ctacaccagg tgaaatagat cacctcccaa
     1141 gtgcatgaga cacgaaccaa ccacttatca tcagatcaca aaaagcctac ttgtacagct
     1201 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt
     1261 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct
     1321 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct
     1381 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct
     1441 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt
     1501 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg
     1561 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     1621 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc
     1681 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca
     1741 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     1801 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga
     1861 tcactgcctc gcccttgctc accatgaatt ctccagctcc agcagaactc gtaaacttcg
     1921 taaccgcctt agtcccttca gaaacagcat gtttcgccaa ctcaccgggc aacacaagtc
     1981 taaccgcagt ctgaatctcc ctggaagtaa tcgtcggctt cttgttgtac ctcgcaagct
     2041 tcgaagactc accagcaagc ttctcaaaga tatcgttgat gaaactgttc atgattccca
     2101 tggctttgct tgagattccg atgtctggat gaacttgctt caacaccttg aagatgtaga
     2161 tcttgtatgt ctcaacgttc ttctttgatc tcttcttctt cttgtctccg gcgacggctg
     2221 gttccttggg gagtttcttt ccggcttttg gtttcttctc tgccgctgct gctggttcga
     2281 ctgccggagt tttctctgcc ggtttcttct ccgctggttt cttatctgcc ttcgccatct
     2341 gttaatcaga aaaactcaga ttaatcgaca aattcgatcg cacaaactag aaactaacac
     2401 cagatctaga tagaaatcac aaatcgaaga gtaattattc gacaaaactc aaattatttg
     2461 aacaaatcgg atgatatcta tgaaacccta atcgagaatt aagatgatat ctaacgatca
     2521 aacccagaaa atcgtcttcg atctaagatt aacagaatct aaaccaaaga acatatacga
     2581 aattgggatc gaacgaaaac aaaatcgaag attttgagag aataaggaac acagaaattt
     2641 accttgatca cggtagagag aattgagaga aagtttttaa gattttgaga aattgaaatc
     2701 tgaattgtga agaagaagag ctctttgggt attgttttat agaagaagaa gaagaaaaga
     2761 cgaggacgac taggtcacga gaaagctaag gcggtgaagc aatagctaat aataaaatga
     2821 cacgtgtatt gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc ttatttttag
     2881 gttgaggaaa agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa
     2941 ccactttgac gccgtttatt actgactcgt cgacaaccac aatttctaac ggtcgtcata
     3001 agatccagcc ggtttgacca ttaccaaacc gacctaacaa caaaggtcca tgaccatgct
     3061 tcgacatgaa ctcaaatgtc ctgatctcat cctcacgatc aaagaaaatc tcaacacctt
     3121 caccataaat cctaaccaaa accttacgag aaggaccttt ttccctagtt ggccatttga
     3181 tctgaaacac ctcatttgtc attgcacctt ttaaaggaat aacttgcaga gcctttgaat
     3241 caatcacatc ttcccactct gatgctattg cttgtaaagc ttccttgact tctcgaggca
     3301 aacgatattg tttattctct acattctttt cagtaccacc catcgtcatt tgaatgccac
     3361 acacaaagat aaatctctct gtacaagata aaaacagata cttttagcga atacttataa
     3421 caacactgtg ttttgtccaa ttcaggggag gttctacgat caacccaatg caacaattct
     3481 atatgcttcc gtttcataaa aattctgaat atcaatctaa acctatacaa ttacatcctt
     3541 actaatctag ataaagtgta tacaataaaa tgagagcaat aaggttcatt aatctttata
     3601 cttagctcct ccacaggacg atatactttg agactgacac aaaacaaaat aaggaagaaa
     3661 gcttacgttt ttgaaggaaa atcacaggag ggacgcggaa gcaagattgg tctgagagga
     3721 aaattgagga agaggggtta atcgagatga aaatggagtt tgaagtaata gagagaacgt
     3781 tgcgcagatc gaggaagaac agaggatagg atcaacacaa ggatcttgtg aaaatgaaaa
     3841 tggctgagga aatgaaggac tatttgttaa ataagatata atattatttc tgaaatattt
     3901 ccatctgact cctttaattt atacaagcct ccttttttgt acatctattt tcagaagatc
     3961 caaataattg tttcttctat ttgtgtattt ttgatattta aacgtaaatc tttggatttt
     4021 gatcaataat agacaattag ccaagttcag ttttcattaa ttagatttat aattttaatt
     4081 atttttagca ttcgtctaaa attaaataat gcggcaaaag aggagaaatt aatttttgtt
     4141 gttattaatt caaagcgtta caaataaaaa aggaaattcc acgctggcaa taaaataagc
     4201 gaaaattcca cgtgacatct acctgtcgga tcaaaaagag tggaattgac atttgtttct
     4261 ctcacactct ctctcgaatt ctctggtagc ttctagttct atgcaaaaaa acgacgatag
     4321 ttctctatct ttccagatga atctccttcc atatacaaaa gcagtcatgc ctcctcgctc
     4381 tctcgcaatt cacaaagtat ccaaacatct aaagttatca attttacaac attaccgcta
     4441 taatctgctt gattctctgc aaaaagagaa gactttttac cgagaagaag tcctctggct
     4501 cattgaagaa actcaacgaa acaaacccag ttctcatata tcgttttaag gtaaatgatc
     4561 gcgacaatct tgttctcatt tgtgtgtttt tgtgtttgtg attagggttt acaaaagata
     4621 ctgagattag tttttttttt tttttttttt tttcagggat tacaaaagct aatcgaagat
     4681 gaatccagag aaatagggat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     4741 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     4801 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     4861 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     4921 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     4981 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5041 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5101 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5161 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5221 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5281 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5341 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5401 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5461 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5521 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5581 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5641 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5701 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5761 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5821 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     5881 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     5941 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     6001 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gacgatatgg
     6061 cgtccgtgcc tccaggtcga atcttcaaac gacgagagga tatggcctcc gtcgctccag
     6121 tattccaggg ttcatcagag tgccagagtc cttcgatagg gacaagacaa ttgcactgat
     6181 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
     6241 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     6301 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     6361 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
     6421 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     6481 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     6541 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     6601 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     6661 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     6721 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     6781 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     6841 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     6901 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     6961 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     7021 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     7081 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     7141 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     7201 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtatg gtccgtcctg
     7261 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc
     7321 gcgaaaactg tggaattggt cagcgttggt gggaaagcgc gttacaagaa agccgggcaa
     7381 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg
     7441 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg
     7501 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga
     7561 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg
     7621 ggaaaagtct acgtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     7681 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     7741 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     7801 ccaaaatttg ttgatgtgca ggtatcaccg tttgtgtgaa caacgaactg aactggcaga
     7861 ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc
     7921 atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc acgccgaaca
     7981 cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg
     8041 ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac
     8101 aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct
     8161 ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt
     8221 gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc
     8281 tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat gcggacttgc
     8341 gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg gactggattg
     8401 gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag
     8461 atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac ctctctttag
     8521 gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg
     8581 gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc
     8641 acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac
     8701 gggaatattt cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca
     8761 cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg
     8821 tgctgtgcct gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag
     8881 agaaggtact ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca
     8941 tcaccgaata cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga
     9001 gtgaagagta tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg
     9061 ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc
     9121 gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tcggcggctt
     9181 ttctgctgca aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca
     9241 aacaatgaat atgaagatga agatgaatat ttggtgtgtc aaataaaaag cttgtgtgct
     9301 taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta
     9361 aatgaatgta agatctcatt ataatgaata aacaaatgtt tctataatcc attgtgaatg
     9421 ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga
     9481 atatgattaa agataagatg ggctcataga gtaaaacgag gcgagggacc tataaacctc
     9541 acttcatcat gctatttcat gatctatttt ataaaataaa gatgtagaaa aaagtaagcg
     9601 taataaccgc aaaacaaatg atttaaaaca tggcacataa tgaggagatt aagttcggtt
     9661 tacgtttatt ttagtactaa ttgtaacgtg agactacgta tcgggaatcg cctaccgggt
     9721 accgagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc
     9961 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
    10021 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
    10081 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
    10141 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
    10201 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
    10261 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
    10321 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
    10381 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
    10441 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
    10501 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
    10561 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
    10621 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
    10681 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc
    10741 tctacaaatc tatctctctc gagctttcgc agatctgtcg atcgaccatg gggattgaac
    10801 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    10861 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt tcggctgtca gcgcaggggc
    10921 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caggacgagg
    10981 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    11041 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    11101 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    11161 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    11221 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    11281 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    11341 tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    11401 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    11461 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    11521 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    11581 tctgagcggg actctggggt tcggatcgat cctctagcta gagtcgatcg acaagctcga
    11641 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
    11701 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
    11761 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
    11821 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
    11881 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
    11941 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
    12001 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
    12061 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
    12121 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
    12181 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
    12241 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
    12301 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
    12361 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
    12421 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
    12481 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
    12541 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
    12601 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
    12661 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
    12721 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
    12781 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
    12841 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
    12901 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
    12961 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
    13021 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
    13081 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
    13141 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    13201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    13261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    13321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    13381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    13441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    13501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    13561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    13621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    13681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    13741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    13801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    13861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    13921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13981 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
    14041 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    14101 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    14161 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    14221 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    14281 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    14341 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    14401 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    14461 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    14521 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    14581 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    14641 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
    14701 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    14761 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    14821 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    14881 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    14941 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    15001 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    15061 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    15121 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    15181 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    15241 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    15301 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    15361 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    15421 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    15481 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    15541 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    15601 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    15661 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    15721 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    15781 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    15841 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
    15901 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    15961 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    16021 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    16081 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    16141 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    16201 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    16261 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    16321 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    16381 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    16441 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    16501 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    16561 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    16621 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    16681 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    16741 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    16801 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    16861 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    16921 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    16981 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    17041 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    17101 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    17161 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    17221 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    17281 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    17341 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    17401 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    17461 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    17521 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    17581 cccgcgccgg cctcctggat gcg
//
