LOCUS       sequence-319113-        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell 
            adenosine base editor expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Brookhouser N, Nguyen T, Tekel SJ, Standage-Beier K, Wang X, Brafman
            DA
  TITLE     A Cas9-mediated adenosine transient reporter enables enrichment of 
            ABE-targeted cells.
  JOURNAL   BMC Biol. 2020 Dec 14;18(1):193. doi: 10.1186/s12915-020-00929-7.
  PUBMED    33317513
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2020-12-14"; volume: "18(1):193. doi"; pages: " 
            10.1186/s12915-020-00929-7"
COMMENT     Sequence Label: pEF-ABEmax
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..1243
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          292..1234
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1191..1211
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1253..1272
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1253..1271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1321..1341
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2530..2553,2554..2556,2557..6630)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2530..6630
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6673..6693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6702..6719
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6742..6759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6748..6972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7043..7059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7043..7059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7056..7078)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7067..7083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7091..7097,7098..7115,7116..7121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7136..7157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7274..7291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7445..8033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7525..7544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8204..8995,8996..9064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8827..8846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9065..9169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9248..9267)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagtcgtga
       61 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
      121 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      361 acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
      421 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
      481 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      541 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      601 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
      661 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      721 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
      781 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
      841 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
      901 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
      961 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1021 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1081 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     1141 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     1201 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggcggcc gctaatacga
     1261 ctcactatag ggagagccgc caccatgaaa cggacagccg acggaagcga gttcgagtca
     1321 ccaaagaaga agcggaaagt ctctgaagtc gagtttagcc acgagtattg gatgaggcac
     1381 gcactgaccc tggcaaagcg agcatgggat gaaagagaag tccccgtggg cgccgtgctg
     1441 gtgcacaaca atagagtgat cggagaggga tggaacaggc caatcggccg ccacgaccct
     1501 accgcacacg cagagatcat ggcactgagg cagggaggcc tggtcatgca gaattaccgc
     1561 ctgatcgatg ccaccctgta tgtgacactg gagccatgcg tgatgtgcgc aggagcaatg
     1621 atccacagca ggatcggaag agtggtgttc ggagcacggg acgccaagac cggcgcagca
     1681 ggctccctga tggatgtgct gcaccacccc ggcatgaacc accgggtgga gatcacagag
     1741 ggaatcctgg cagacgagtg cgccgccctg ctgagcgatt tctttagaat gcggagacag
     1801 gagatcaagg cccagaagaa ggcacagagc tccaccgact ctggaggatc tagcggagga
     1861 tcctctggaa gcgagacacc aggcacaagc gagtccgcca caccagagag ctccggcggc
     1921 tcctccggag gatcctctga ggtggagttt tcccacgagt actggatgag acatgccctg
     1981 accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg
     2041 aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc
     2101 catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt
     2161 gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt gcgccggcgc catgatccac
     2221 tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc
     2281 ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc
     2341 ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc
     2401 aatgctcaga agaaggccca gagctccacc gactccggag gatctagcgg aggctcctct
     2461 ggctctgaga cacctggcac aagcgagagc gcaacacctg aaagcagcgg gggcagcagc
     2521 ggggggtcag acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
     2581 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     2641 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     2701 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     2761 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     2821 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     2881 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     2941 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     3001 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     3061 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     3121 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     3181 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     3241 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     3301 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     3361 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     3421 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     3481 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     3541 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     3601 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     3661 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     3721 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     3781 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     3841 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     3901 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     3961 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     4021 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     4081 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     4141 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     4201 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     4261 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     4321 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     4381 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     4441 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     4501 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     4561 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     4621 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     4681 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     4741 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     4801 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     4861 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     4921 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     4981 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     5041 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     5101 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     5161 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     5221 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     5281 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     5341 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     5401 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     5461 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     5521 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     5581 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     5641 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     5701 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     5761 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     5821 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     5881 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     5941 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     6001 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     6061 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     6121 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     6181 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     6241 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     6301 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     6361 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     6421 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     6481 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     6541 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     6601 cggatcgacc tgtctcagct gggaggtgac tctggcggct caaaaagaac cgccgacggc
     6661 agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg tcatcatcac catcaccatt
     6721 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6781 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6841 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6901 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6961 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta
     7021 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7081 caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag
     7141 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7201 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9061 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9121 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9181 gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga
     9241 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9301 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9361 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgc
//
