LOCUS       sequence-319119-       12961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitously weakly expressed 120-meric GFP nanocage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 12961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UBC1cer120G
FEATURES             Location/Qualifiers
     source          1..12961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1943..2653
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             3341..4051
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4699..4715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4712..4734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4723..4739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4747..4753,4754..4771,4772..4777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5004..5680
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     5617..5637
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5747..6772
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    6813..6987
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7065..7089
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7514..8308
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8395..8983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8884..8903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9137..9154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9169..9309
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9235..9254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9395..9417
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9653..9847
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9913..10986)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11415..12044)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccgttt tgctgatagg tttcgtcagg attgatatat gttcgtaggt agcagaagag
      721 actaaaagta gtttattttt tcgtcctttt gaccaaatgt tcaaaagcca catatcattt
      781 cgtggtaagc ggttgtatct caaaacatca gtctgatctt agttgttttt gatttaagag
      841 caaatctcaa tttgaagtct gagtacgagc tagtatgatc caaatgccta ggctttgaga
      901 cgaggcttga agatgaattg gtttgtctag agagattgga gaaacaagac ataccacaaa
      961 aacatttctc tctagtaaca aaaacatcac tctactctac tcttgtggtt tgggtctgga
     1021 ttttagtccg gttagtggta caatggttta tattggttta ttagaaatat atagtttacg
     1081 ctacattaag aaaagtactc taatttgtca tctgaagatt tacataaaaa agattacatg
     1141 tcatcgttgt atgttaacta acactaatct cccggttaaa gtgtagattg ccctaaacgg
     1201 tttaattccg gttaggaaca aaccaaccac tcgctcggtc gtccaaaggg caaagcgaat
     1261 ccaactttac agatatgtca gagaggagcc aagcttctag aagatcattc attcatcact
     1321 tatacgatct cttcccaaat tattgatttt ttttacccat ttctctcatc gcacggtgat
     1381 attgagaatc gccgacctga atcgatcgga aaactttctc tgattaccgg cggtcaacac
     1441 cgctgaacac atatgtttgt ttgacgacct cttctctccg cgatctttac ctcaacaacg
     1501 agatctgttt ccacggtacc attttcatcc gatctctgtt cttctatgtt tgtttactgg
     1561 atccgttgat tttactttca tcttatcatt tgtgtgtggc tattttggtg aattgatgcg
     1621 atcttaagct gtttctaggt tcaagcctta tatgtttctt ctaagctaat cataggtcgt
     1681 agacttgtta ggaagattcg aatctagtac aaagttagca actttagcat acgtaaatcg
     1741 ttagtagtaa tttctagatt gatagaggtt cgagacctta aaaggtgatg aatatttggt
     1801 gggttttgtt tattagaaag aaaggaggat gatggatccg gtagtagtgc tgggtttgtg
     1861 tttgagctgt ctcctgttgc tgagtctctg gaagcaatcc tacggcggtg gcaaactggg
     1921 cggaggtggc ggtggagcta gcagtaaagg agaagaactt ttcactggag ttgtcccaat
     1981 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     2041 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     2101 tgttccgtgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcaagata
     2161 cccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg gatacgtgca
     2221 ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg aagtcaagtt
     2281 tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca aggaggacgg
     2341 aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat acatcatggc
     2401 cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca tcgaagacgg
     2461 cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg gccctgtcct
     2521 tttaccagac aaccattacc tgtccacaca atctaagctt tcgaaagatc ccaacgaaaa
     2581 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2641 tgaactatac aaaggaggca gcggcggatc aggagggtca aaaatggagg aactctttaa
     2701 aaagcacaag atcgtggcag tactccgggc caatagcgta gaggaggcta agaaaaaagc
     2761 tctcgctgtg tttctgggcg gcgtgcactt gatcgagata acttttacgg ttcctgacgc
     2821 cgatacggtc atcaaagaac tgagtttttt gaaagaaatg ggagcaataa tcggagccgg
     2881 cacagttacg tccgtggaac agtgtcggaa agcagttgag tcgggcgcgg agtttatcgt
     2941 tagtccgcat ctcgacgagg agatcagcca attctgcaaa gagaaaggag tattttacat
     3001 gcccggcgtg atgacaccga ctgagctggt caaagctatg aagctgggcc acactatact
     3061 cgccctgttt ccaggagaag ttgttggccc tcaattcgta aaagcgatga agggtccctt
     3121 ccccaacgta aaattcgttc caacgggcgg agtcaacctg gataacgtat gcgagtggtt
     3181 taaagccggc gttttggctg ttggtgtagg aagtgctctc gttaaaggta cgcctgtcga
     3241 agtggctgag aaagctaaag cctttgttga aaagatacgt ggctgtaccg aaggaggcag
     3301 cggcggatca ggagggtcag gtggatcagt agatctgact agtaaaggag aagaactttt
     3361 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     3421 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     3481 cactactgga aaactacctg ttccgtggcc aacacttgtc actactttct cttatggtgt
     3541 tcaatgcttt tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat
     3601 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac
     3661 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat
     3721 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca
     3781 caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg
     3841 ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat
     3901 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctaagctttc
     3961 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     4021 gattacacat ggcatggatg aactatacaa agctagccca tggtaagctt tttgtgatct
     4081 gatgataagt ggttggttcg tgtctcatgc acttgggagg tgatctattt cacctggtgt
     4141 agtttgtgtt tccgtcagtt ggaaaaactt atccctatcg atttcgtttt cattttctgc
     4201 ttttctttta tgtaccttcg tttgggcttg taacgggcct ttgtatttca actctcaata
     4261 ataatccaag tgcatgttaa acaatttgtc atctgtttcg gctttgatat actactggtg
     4321 aagatgggcc gtactactgc atcacaacga aaaataataa taagatgaaa aacttgaagt
     4381 ggaaaaaaaa aaaacttgaa tgttcactac tactcattga ccataatgtt taacatacat
     4441 agctcaatag tatttttgtg aatatggcaa cacaaacagt ccaaaacaat tgtctcttac
     4501 tataccaaac caagggcgcc gcttgtttgc cactctttgt gtgcaatagt gtgattacca
     4561 catctccaca ttcaatatat tccctgaatt atctgacgat tttgatggct cactgttttc
     4621 ccaagtctgg taccccacct acatggggat cctaactcga gctgcaggct tcggtaccga
     4681 gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag
     4921 cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     4981 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     5041 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     5101 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     5161 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     5221 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
     5281 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     5341 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     5401 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     5461 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     5521 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     5581 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     5641 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
     5701 aaatctatct ctctcgagct ttcgcagatc cggggggcaa tgagatatga aaaagcctga
     5761 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     5821 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     5881 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     5941 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga
     6001 gagcctgacc tattgcatct cccgccgttc acagggtgtc acgttgcaag acctgcctga
     6061 aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc
     6121 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     6181 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     6241 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     6301 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     6361 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     6421 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     6481 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta
     6541 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     6601 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     6661 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     6721 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agagtagatg
     6781 ccgaccggga tctgtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc
     6841 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     6901 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     6961 ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt
     7021 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     7081 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     7141 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     7201 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     7261 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     7321 gtgatcaccg cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     7381 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     7441 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     7501 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     7561 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     7621 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     7681 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     7741 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     7801 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     7861 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     7921 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     7981 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     8041 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     8101 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     8161 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     8221 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     8281 ttatatttta ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg
     8341 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8401 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8461 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8521 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8581 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8641 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8701 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8761 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8821 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8881 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8941 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9001 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9061 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9121 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     9181 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     9241 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     9301 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9361 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     9421 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     9481 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     9541 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     9601 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     9661 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     9721 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     9781 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     9841 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     9901 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     9961 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
    10021 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
    10081 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
    10141 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
    10201 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
    10261 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
    10321 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
    10381 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
    10441 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
    10501 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
    10561 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
    10621 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
    10681 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
    10741 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
    10801 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
    10861 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
    10921 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
    10981 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc
    11041 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
    11101 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
    11161 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
    11221 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
    11281 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
    11341 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
    11401 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
    11461 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
    11521 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
    11581 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
    11641 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
    11701 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
    11761 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
    11821 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    11881 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    11941 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    12001 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    12061 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    12121 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    12181 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    12241 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    12301 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    12361 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    12421 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    12481 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    12541 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    12601 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    12661 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    12721 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    12781 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    12841 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    12901 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    12961 g
//
