LOCUS       sequence-319125-       13530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  module containing the human codon optimized Cas9 and the BASTA 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13530)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos...
FEATURES             Location/Qualifiers
     source          1..13530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(41..293)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(313..864)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(906..1085)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1791..2135
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2079..2099
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2209..6312
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2209..6312
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2209..6312
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6325..6345
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6372..6624
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6659..6677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6866..6890
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8191..8820
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9249..10322
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10388..10582
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10818..10840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10926..11066
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10981..11000
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11081..11098)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11252..11840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11332..11351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11928..12719)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13505..13529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacggatcc ggtctcagga gatcagcttg catgccggtc gatctagtaa catagatgac
       61 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
      121 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
      181 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcccaaga
      241 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga
      301 ctctaggggt catcagattt cggtgacggg caggaccgga cggggcggca ccggcaggct
      361 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      421 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      481 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      541 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggacacgt acacggtcga
      601 ctcggccgtc cagtcgtagg cgttgcgtgc cttccaggga cccgcgtagg ctatgccggc
      661 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      721 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      781 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      841 ggccgggcgt cgttctgggc tcatggtaga tcccctcgat cgagttgaga gtgaatatga
      901 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
      961 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     1021 gtatgtgctt agctcattaa actccagaaa cccgcggctc agtggctcct tcaacgttgc
     1081 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     1141 tgactccctt aattctcatg tatgatacgc tgtcaggaga ctagagccaa gctgatctcc
     1201 tttgccccgg agatcaccat ggacgacttt ctctatctct acgatctagg aagaaagttc
     1261 gacggagaag gtgacgatac catgttcacc accgataatg agaagattag cctcttcaat
     1321 ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcggcagg tctgatcaag
     1381 acgatctacc cgagtaataa tctccaggag atcaaatacc ttcccaagaa ggttaaagat
     1441 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag
     1501 atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta
     1561 atagagattg gagtctctaa gaaagtagtt cctactgaat caaaggccat ggagtcaaaa
     1621 attcagatcg aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt
     1681 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctc
     1741 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     1801 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     1861 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     1921 aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc cccacccacg
     1981 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     2041 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     2101 tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca acaattacca
     2161 caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgaaat ggacaagaag
     2221 tactccattg ggctcgatat cggcacaaac agcgtcggct gggccgtcat tacggacgag
     2281 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
     2341 aagaacctca ttggcgccct cctgttcgac tccggggaaa cggccgaagc cacgcggctc
     2401 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
     2461 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
     2521 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
     2581 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
     2641 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
     2701 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
     2761 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
     2821 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
     2881 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
     2941 tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt
     3001 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
     3061 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat
     3121 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc
     3181 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
     3241 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
     3301 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
     3361 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact
     3421 ttcgacaatg gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
     3481 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
     3541 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg
     3601 atgactcgca aatcagaaga gactatcact ccctggaact tcgaggaagt cgtggataag
     3661 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
     3721 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
     3781 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag
     3841 aagaaagcta tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
     3901 aaagaagatt atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
     3961 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
     4021 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
     4081 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
     4141 gacaaagtca tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga
     4201 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
     4261 tccgatggat ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt
     4321 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtctcca cgagcacatc
     4381 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
     4441 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
     4501 cgagagaacc aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
     4561 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
     4621 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
     4681 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
     4741 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaataga
     4801 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
     4861 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
     4921 cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca
     4981 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
     5041 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtttca
     5101 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
     5161 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
     5221 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
     5281 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
     5341 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
     5401 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
     5461 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
     5521 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
     5581 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
     5641 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
     5701 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
     5761 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
     5821 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac
     5881 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
     5941 ctcaaaggat ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
     6001 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
     6061 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
     6121 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
     6181 aagtacttcg acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
     6241 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
     6301 ctcggtggag acagcagggc tgaccccaag aagaagagga aggtgtgagc ttggaatgga
     6361 tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     6421 ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6481 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6541 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6601 cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc
     6661 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6721 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6781 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga
     6841 ctcgcgctgt catgagaccg gatcctgaca ggatatattg gcgggtaaac ctaagagaaa
     6901 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     6961 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     7021 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
     7081 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     7141 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     7201 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     7261 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     7321 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     7381 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     7441 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     7501 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     7561 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     7621 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     7681 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     7741 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     7801 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     7861 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     7921 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     7981 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     8041 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     8101 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     8161 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     8221 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     8281 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     8341 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     8401 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     8461 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     8521 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     8581 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     8641 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     8701 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     8761 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     8821 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     8881 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     8941 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     9001 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     9061 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     9121 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     9181 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     9241 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     9301 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     9361 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     9421 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9481 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9541 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     9601 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     9661 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     9721 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     9781 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     9841 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     9901 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     9961 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    10021 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    10081 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    10141 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    10201 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    10261 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    10321 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
    10381 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    10441 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    10501 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    10561 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    10621 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    10681 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    10741 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    10801 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
    10861 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    10921 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    10981 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    11041 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    11101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    11161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    11221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    11701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11881 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta tttgccgact
    11941 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
    12001 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
    12061 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
    12121 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcacca
    12181 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
    12241 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
    12301 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
    12361 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
    12421 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
    12481 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
    12541 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
    12601 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
    12661 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
    12721 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
    12781 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
    12841 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    12901 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta
    12961 cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt
    13021 cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggaacagaac
    13081 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    13141 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    13201 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    13261 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    13321 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    13381 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    13441 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    13501 ctggtggcag gatatattgt ggtgtaaaca
//
