LOCUS       sequence-319128-       13449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a
            EB...
FEATURES             Location/Qualifiers
     source          1..13449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3139..3148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3145..3843
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3894..4118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5167..6349
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5398..6340
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6450..6459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6459..6524
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6531..6551
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7148..7372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8421..9603
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8652..9594
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9704..9713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9710..9712,9713..9715,9716..10426)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9755..9776)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10454..10510
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10523..10921
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10972..11196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12055..12864)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12778..12797
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12856..12875
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(13023..13449,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13103..13122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattacga tcggagtgag
     3001 gataattaga gtgaggacga tcggagtgag gaaaattaga gtgaggacga tcggagtgag
     3061 gataattaga gtgaggacga tccctctaga gggtatataa tgggggccac tagtctacta
     3121 ccagagctca tcgctagcgc caccatggtg agcgagctga ttaaggagaa catgcacatg
     3181 aagctgtata tggagggcac cgtgaacaac caccacttca agtgtacctc cgagggcgaa
     3241 ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg cggccctctc
     3301 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac
     3361 cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt cacatgggag
     3421 agagtcacca catacgaaga tgggggcgtg ctgaccgcta cccaggacac cagcctccag
     3481 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
     3541 gtgatgcaga agaaaacact cggctgggag gcctccaccg aaaccctgta ccccgctgac
     3601 ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
     3661 tgcaacttga aaaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     3721 gtctactatg tggacaggag actggaaaga atcaaggagg ccgacaaaga aacctacgtc
     3781 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga
     3841 tgagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt
     4081 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcag gtcgagggta
     4141 catgcgcctt actccttgtg agcgcatcgc gataagctct agagggacag ccccccccca
     4201 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     4261 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
     4321 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     4381 agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga
     4441 atgacagaat catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc
     4501 gaattcctgc agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta
     4561 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
     4621 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
     4681 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
     4741 atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc
     4801 ctgggcgcgc ctggtggcga acgatcaaca gacgggagag ggcaaaaccg atgttgacgg
     4861 actaatcctg acaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     4921 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     4981 gctgcttcgc gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa aggactcgcc
     5041 cctgccttgg ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat
     5101 cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat
     5161 gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5221 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     5281 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5341 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     5401 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     5461 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     5521 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     5581 tgaggcctgg cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     5641 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     5701 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     5761 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     5821 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     5881 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     5941 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     6001 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     6061 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     6121 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     6181 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     6241 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     6301 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agctcgttta
     6361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagagac
     6421 cgggaccgat ccaaattctc gaggctagcg ccaccatgga ttataaggat cacgatggcg
     6481 actacaagga ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa
     6541 agcgcaaagt cggtatccat ggcgttcccg tcgacggtgg actgcaggcc ccaccaaccg
     6601 atgtttcttt aggtgatgag ttacatttgg acggtgagga cgtagctatg gcacatgcag
     6661 atgccttaga cgattttgac ttggatatgt taggagatgg agattcacca ggacctggtt
     6721 tcacaccaca cgattcagct ccatacggtg ctttggacat ggccgatttc gaatttgaac
     6781 agatgtttac tgatgccttg ggtatagacg aatacggtgg tggagctaga cccggggagc
     6841 gccccttcca gtgtcgcatt tgcatgcgga acttttcgcg ccaggacagg cttgacaggc
     6901 atacccgtac tcataccggt gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct
     6961 cccagaagga gcacttggcg gggcatctac gtacgcacac cggcgagaag ccattccaat
     7021 gccgaatatg catgcgcaac ttcagtcgcc gcgacaacct gaaccggcac ctaaaaaccc
     7081 acctgagagg atcttaagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     7141 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7201 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7261 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7321 gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7381 gcaggtcgaa gaactatttc ctggctgtta cgcgagcgca tcgcgataag ctctagaggg
     7441 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     7501 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     7561 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     7621 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     7681 aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg gctctagctc
     7741 tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc ccaaagcccc
     7801 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     7861 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     7921 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     7981 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     8041 aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg agaggactaa
     8101 atagtagttc agacgccgtt aagcgcaaga tctggggagg tcgctgagta gtgcgcgagc
     8161 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     8221 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtga gtaattcata
     8281 caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg
     8341 tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag
     8401 gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     8461 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     8521 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     8581 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     8641 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     8701 gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc
     8761 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     8821 cctcgtgctt gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg
     8881 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     8941 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     9001 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     9061 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     9121 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     9181 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     9241 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     9301 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     9361 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     9421 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     9481 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     9541 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     9601 tgagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     9661 tccatagaag agaacgggac cgatccaaat tctcgaggct agcgccacca tggtgagcaa
     9721 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     9781 cggccacaag ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac
     9841 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     9901 cctgagccac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     9961 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
    10021 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat
    10081 cgagctgaag ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
    10141 caacttcaac agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt
    10201 gaacttcaag atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca
    10261 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac
    10321 ccagtccgtg ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
    10381 ccgcaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaat tcggtagcaa
    10441 gcttggtagt ggagcaacta acttttccct gctcaaacaa gccggggatg tagaggagaa
    10501 ccccggcccc gcccccctta agatggccaa gcctttgtct caagaagaat ccaccctcat
    10561 tgaaagagca acggctacaa tcaacagcat ccccatctct gaggactaca gcgtcgccag
    10621 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
    10681 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
    10741 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
    10801 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
    10861 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
    10921 agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
    10981 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
    11041 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
    11101 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca
    11161 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcaggt cgaagagact
    11221 gcccgacaca tcttagtgag cgcatcgcga taagctctag agggacagcc cccccccaaa
    11281 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
    11341 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
    11401 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
    11461 cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat
    11521 gacagaatca tagaacggcc tgcgtacgga tggggctcta gctctagagc ttgatatcga
    11581 attcctgcag ccccgctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
    11641 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
    11701 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
    11761 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
    11821 acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct
    11881 gggcgcgcct ggtggcgaac gatcaacaga cgggagaggt tacgaggatg cacatgtgac
    11941 cgagggatgg aacgacgcgt aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc
    12001 ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa
    12061 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
    12121 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
    12181 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
    12241 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
    12301 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
    12361 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
    12421 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
    12481 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
    12541 taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
    12601 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
    12661 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
    12721 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
    12781 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
    12841 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
    12901 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
    12961 cacgggccat gctagctcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    13021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    13081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    13141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    13201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    13261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    13321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13441 cctaactac
//
