LOCUS       sequence-319130-       13506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a
            E...
FEATURES             Location/Qualifiers
     source          1..13506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3196..3205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3202..3900
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3951..4175
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5224..6406
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5455..6397
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6507..6516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6516..6581
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6588..6608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7205..7429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8478..9660
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8709..9651
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9761..9770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9767..9769,9770..9772,9773..10483)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9812..9833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10511..10567
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10580..10978
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11029..11253
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12112..12921)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12835..12854
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12913..12932
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(13080..13506,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13160..13179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg gagtgaggaa aattagagtg aggacgatcg gagtgaggat
     3001 aattagagtg aggacgatcg gagtgaggaa aattagagtg aggacgatcg gagtgaggat
     3061 aattagagtg aggacgatcg gagtgaggaa aattagagtg aggacgatcg gagtgaggat
     3121 aattagagtg aggacgatcc ctctagaggg tatataatgg gggccactag tctactacca
     3181 gagctcatcg ctagcgccac catggtgagc gagctgatta aggagaacat gcacatgaag
     3241 ctgtatatgg agggcaccgt gaacaaccac cacttcaagt gtacctccga gggcgaaggc
     3301 aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc
     3361 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac
     3421 acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga
     3481 gtcaccacat acgaagatgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     3541 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
     3601 atgcagaaga aaacactcgg ctgggaggcc tccaccgaaa ccctgtaccc cgctgacggc
     3661 ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc
     3721 aacttgaaaa ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
     3781 tactatgtgg acaggagact ggaaagaatc aaggaggccg acaaagaaac ctacgtcgag
     3841 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacagatga
     3901 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3961 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4021 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4081 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagtcaa
     4141 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcaggtc gagggtacat
     4201 gcgccttact ccttgtgagc gcatcgcgat aagctctaga gggacagccc ccccccaaag
     4261 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     4321 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     4381 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     4441 ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg
     4501 acagaatcat agaacggcct gcgtacggat ggggctctag ctctagagct tgatatcgaa
     4561 ttcctgcagc cccgctagag ggacagcccc cccccaaagc ccccagggat gtaattacgt
     4621 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
     4681 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     4741 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
     4801 cggggaaaaa gctttaggct gaaagagaga tttagaatga cagaatcata gaacggcctg
     4861 ggcgcgcctg gtggcgaacg atcaacagac gggagagggc aaaaccgatg ttgacggact
     4921 aatcctgaca agatctgggg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     4981 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     5041 gcttcgcgat gtacgggcca gatatacgcg tgagtaattc atacaaaagg actcgcccct
     5101 gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc
     5161 tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg
     5221 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5281 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5341 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5401 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     5461 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     5521 attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     5581 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     5641 ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     5701 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     5761 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     5821 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     5881 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     5941 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     6001 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     6061 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     6121 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     6181 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     6241 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     6301 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     6361 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagagc tcgtttagtg
     6421 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagagaccgg
     6481 gaccgatcca aattctcgag gctagcgcca ccatggatta taaggatcac gatggcgact
     6541 acaaggacca cgatattgac tacaaagacg atgacgataa aatggctcct aagaaaaagc
     6601 gcaaagtcgg tatccatggc gttcccgtcg acggtggact gcaggcccca ccaaccgatg
     6661 tttctttagg tgatgagtta catttggacg gtgaggacgt agctatggca catgcagatg
     6721 ccttagacga ttttgacttg gatatgttag gagatggaga ttcaccagga cctggtttca
     6781 caccacacga ttcagctcca tacggtgctt tggacatggc cgatttcgaa tttgaacaga
     6841 tgtttactga tgccttgggt atagacgaat acggtggtgg agctagaccc ggggagcgcc
     6901 ccttccagtg tcgcatttgc atgcggaact tttcgcgcca ggacaggctt gacaggcata
     6961 cccgtactca taccggtgaa aaaccgtttc agtgtcggat ctgtatgcga aatttctccc
     7021 agaaggagca cttggcgggg catctacgta cgcacaccgg cgagaagcca ttccaatgcc
     7081 gaatatgcat gcgcaacttc agtcgccgcg acaacctgaa ccggcaccta aaaacccacc
     7141 tgagaggatc ttaagcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     7201 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7261 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7321 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7381 gattgggaag tcaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggca
     7441 ggtcgaagaa ctatttcctg gctgttacgc gagcgcatcg cgataagctc tagagggaca
     7501 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     7561 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     7621 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     7681 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
     7741 agagatttag aatgacagaa tcatagaacg gcctgcgtac ggatggggct ctagctctag
     7801 agcttgatat cgaattcctg cagccccgct agagggacag ccccccccca aagcccccag
     7861 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     7921 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     7981 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     8041 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat
     8101 catagaacgg cctgggcgcg cctggtggcg aacgatcaac agacgggaga ggactaaata
     8161 gtagttcaga cgccgttaag cgcaagatct ggggaggtcg ctgagtagtg cgcgagcaaa
     8221 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     8281 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtgagta attcatacaa
     8341 aaggactcgc ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag
     8401 aacccagaga tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg
     8461 gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     8521 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     8581 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     8641 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     8701 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     8761 cttgcgtgcc ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag
     8821 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     8881 cgtgcttgag ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     8941 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     9001 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     9061 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     9121 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     9181 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     9241 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     9301 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     9361 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     9421 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     9481 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     9541 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     9601 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     9661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     9721 atagaagaga acgggaccga tccaaattct cgaggctagc gccaccatgg tgagcaaggg
     9781 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     9841 ccacaagttc agcgtgaggg gcgagggcga gggcgatgcc accaacggca agctgaccct
     9901 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     9961 gagccacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
    10021 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
    10081 cacctacaag acccgcgccg aggtgaagtt cgagggcgac accctagtga accgcatcga
    10141 gctgaagggc gtcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
    10201 cttcaacagc cacaacatct atatcatggc cgtcaagcag aagaacggca tcaaggtgaa
    10261 cttcaagatc cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca
    10321 gaacaccccc atcggcgacg gccccgtgct gctgcccgac agccactacc tgagcaccca
    10381 gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttccg
    10441 caccgccgcc gggatcactc tcggcatgga cgagctgtac aaggaattcg gtagcaagct
    10501 tggtagtgga gcaactaact tttccctgct caaacaagcc ggggatgtag aggagaaccc
    10561 cggccccgcc ccccttaaga tggccaagcc tttgtctcaa gaagaatcca ccctcattga
    10621 aagagcaacg gctacaatca acagcatccc catctctgag gactacagcg tcgccagcgc
    10681 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
    10741 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
    10801 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
    10861 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
    10921 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
    10981 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
    11041 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
    11101 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
    11161 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagtcaata
    11221 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcaggtcga agagactgcc
    11281 cgacacatct tagtgagcgc atcgcgataa gctctagagg gacagccccc ccccaaagcc
    11341 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
    11401 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
    11461 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
    11521 gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac
    11581 agaatcatag aacggcctgc gtacggatgg ggctctagct ctagagcttg atatcgaatt
    11641 cctgcagccc cgctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
    11701 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
    11761 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
    11821 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
    11881 gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg
    11941 cgcgcctggt ggcgaacgat caacagacgg gagaggttac gaggatgcac atgtgaccga
    12001 gggatggaac gacgcgtaac tcacgttaag ggattttggt catgagcttg cgccgtcccg
    12061 tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa
    12121 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
    12181 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
    12241 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
    12301 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
    12361 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
    12421 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
    12481 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
    12541 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
    12601 tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt
    12661 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
    12721 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
    12781 cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg
    12841 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt
    12901 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
    12961 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
    13021 gggccatgct agctccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    13081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    13141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    13201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    13261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    13321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    13381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    13441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    13501 aactac
//
